StarBright Blue Dyes

Excitable by the 488 nm laser, StarBright Blue (SBB) Dyes are perfect to replace existing dyes in your flow cytometry panels and expand your capabilities across the blue laser.

Designed to be bright enough to resolve most populations, with reduced excitation by the 561 and 640 nm lasers, StarBright Blue Dyes should be your first choice in panel design.

SBB Dye Range

StarBright Dye

Max Ex, nm

Max Em, nm

Brightness (1-5)

5-Laser ZE5 Cell Analyzer Optimal Filter

3/4-Laser ZE5 Cell Analyzer Optimal Filter

Comparison Dye

Launch Date

Flier

StarBright Blue 520 Dye

TBD

TBD

TBD

525/35

509/24

FITC, A488, BB515

Coming soon

 

StarBright Blue 580  Dye

475

580

4

593/52

583/30

-

Coming soon

 

StarBright Blue 615  Dye

475

611

3

593/52

615/24

-

Coming soon

 

StarBright Blue 675  Dye

474

675

5

692/80

692/80

PerCP

Coming soon

 

StarBright Blue 700  Dye

470

705

5

692/80

692/80

PerCP-Cy5.5, BB700, PerCP-eFluor710

Available now

SBB700 PDF

StarBright Blue 765  Dye

474

763

4

750LP

750LP

-

Coming soon

 

StarBright Blue 810  Dye

475

808

3

750LP

750LP

-

Coming soon

 

Abbreviations: Axxx, Alexa Fluor; BB, Brilliant Blue; Cy, cyanine; FITC, fluorescein isothiocyanate; PerCP, peridinin chlorophyll protein.
Fig. 1. Excitation and emission spectra of StarBright Blue Dyes.


Fig. 1. Excitation and emission spectra of StarBright Blue Dyes.


Fig. 2. Comparison of the brightness of SBB700 with PerCP-Cy5.5 and BB700.

Fig. 2. Comparison of the brightness of SBB700 with PerCP-Cy5.5 and BB700. Mouse blood stained with CD4 PerCP-Cy5.5 and CD4 BB700 compared with CD4 SBB700 shows the increased stain index and brightness of the new StarBright Dye.

StarBright Blue 700 Dye

StarBright Blue 700 (SBB700) Dye is the perfect replacement for PerCP, PerCP based-tandem dyes, such as PerCP-Cy5.5, and Brilliant Blue 700 (BB700) in your flow cytometry experiments. It is as bright as BB700 and brighter than PerCP-Cy5.5 (Figure 2). Furthermore, SBB700 has a unique spectral profile allowing it to be combined with PerCP-Cy5.5 or BB700 in full spectrum flow cytometry (Figure 3).

In addition to the increased brightness, SBB700 has reduced excitation by the 640 nm laser and therefore less compensation is required when combining with A647 and A700 in multicolor panels compared with PerCP-Cy5.5 and BB700.

Relatively high levels of compensation may be required when combining SBB700 with StarBright Violet 710 (SBV710) Dye, leading to spreading with values of ~2. While this is not a major issue, we would recommend using mutually exclusive markers for this combination in conventional flow. This combination has a similarity score of 0.64 in spectral flow cytometry (determined using SpectroFlo Software) and is therefore an excellent combination to use.

Fig. 3. SBB700 in full spectrum flow cytometry.


Fig. 3. SBB700 in full spectrum flow cytometry. A, the full emission spectrum of SBB700 showing the emission at all wavelengths. B, human peripheral blood monocytes were stained for CD16 PerCP-Cy5.5 and CD14 SBB700 (MCA1568SBB700) to reveal classical, intermediate, and nonclassical monocyte populations. Data were collected on the Cytek Aurora Flow Cytometry System using SpectroFlo Software.


Fig. 4. SBB Dyes are compatible with PE tandem dyes.

Fig. 4. SBB Dyes are compatible with PE tandem dyes. Human peripheral blood was stained with Mouse Anti-Human CD45PE-A750 (MCA87P750) and Mouse Anti-Human CD4SBB765.

More Dyes Coming Soon

We will be expanding the StarBright Blue Dye range with six more dyes coming soon.

Designed to be compatible with PE and PE tandem dyes with similar maximal emissions (Figure 4), these dyes will provide additional choice and flexibility when building multicolor panels in both conventional and full spectrum flow cytometry and have been validated in both.

StarBright Blue Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    Associated Reading

    For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our StarBright Dyes page.

    Useful information about fluorescent dyes and immunophenotyping can be found in our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our Flow Cytometry Basics Guide.