StarBright Blue Dyes

SBB580 Dye is a bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry (Figure 2).

It is brighter than other 488 nm laser excitable dyes such as AF532, and is as bright as PE when excited by the 488 nm laser.

It has the added benefit of having reduced excitation by the 561 nm laser compared with PE. SBB580 and PE are compatible in a panel when using both the 488 and 561 nm lasers to excite each respective dye.

This provides more choice and flexibility when building multicolor panels.

SBB615 Dye is a bright, 488 nm laser excitable dye with a unique, narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry (Figure 3).

It is brighter than PE-CF594 and PE-Dazzle594 when excited by the 488 nm and is not excited to the same degree by the 561 nm laser.

As it is not a traditional tandem dye, SBB615 does not have issues around tandem breakdown and changes to staining over time. SBB615 provides more choice and flexibility when building multicolor panels incorporating the 488 nm laser.

SBB675 Dye is a very bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry (Figure 4).

It is brighter than other 488 nm laser excitable dyes such as PerCP-Cy5.5 (and PerCP) and BB700 and PE-Cy5 when excited by the 488 nm laser. As it is not a traditional tandem dye, SBB675 does not have issues around tandem breakdown and changes to staining over time.

It exhibits reduced excitation by the 561 nm laser compared to PE tandem dyes. SBB675 provides more choice and flexibility when building multicolor panels incorporating the 488 nm laser.

SBB700 Dye is the perfect replacement for PerCP, PerCP-based-tandem dyes, such as PerCP-Cy5.5, and Brilliant Blue 700 (BB700) in your flow cytometry experiments. It is as bright as BB700 and brighter than PerCP-Cy5.5 (Figure 5). Furthermore, SBB700 has a unique spectral profile allowing it to be combined with PerCP-Cy5.5 or BB700 in full-spectrum flow cytometry (Figure 6).

In addition to the increased brightness, SBB700 has reduced excitation by the 640 nm laser, and therefore less compensation is required when combining with A647 and A700 in multicolor panels compared with PerCP-Cy5.5 and BB700. Relatively high levels of compensation may be required when combining SBB700 with StarBright Violet 710 (SBV710) Dye, leading to spreading with values of ~2.

While this is not a major issue, we would recommend using mutually exclusive markers for this combination in conventional flow. This combination has a similarity score of 0.64 in spectral flow cytometry (determined using SpectroFlo Software) and is, therefore an excellent combination to use.

Fig. 6. SBB700 in full spectrum flow cytometry. A, the full emission spectrum of SBB700 showing the emission at all wavelengths. B, human peripheral blood monocytes were stained for CD20 PerCP-Cy5.5 and CD45RO SBB700 (MCA461SBB700) to reveal classical, intermediate, and nonclassical monocyte populations. Data were collected on the Cytek Aurora Flow Cytometry System using SpectroFlo Software. 

SBB765 Dye is a bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry (Figure 7).

It is brighter than PE-Cy7 when excited by the 488 nm laser. As it is not a traditional tandem dye, SBB765 does not have issues around tandem breakdown and changes to staining over time and exhibits reduced excitation by the 561 nm laser compared to PE tandem dyes.

SBB765 provides more choice and flexibility when building multicolor panels incorporating the 488 nm laser.

SBB810 Dye is a bright, 488 nm laser excitable dye with a unique narrow excitation and emission profile and is suitable for conventional and full spectrum flow cytometry (Figure 8).

It is brighter than PE-Cy7 when excited by the 488 nm laser. As it is not a traditional tandem dye, SBB810 does not have issues around tandem breakdown and changes to staining over time and exhibits reduced excitation by the 561 nm laser compared to PE tandem dyes.

SBB810 provides more choice and flexibility when building multicolor panels incorporating the 488 nm laser.