VEGF antibody
Rabbit anti Mouse VEGF antibody recognizes mouse Vascular Endothelial Growth Factor (VEGF), a protein active in angiogenesis, vasculogenesis and endothelial cell growth.
Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species.
Rabbit anti Mouse VEGF antibody has not been cross adsorbed and may react with homologous proteins from related species.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to mouse VEGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant mouse VEGF
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution.
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- Q00731 Related reagents
- Entrez Gene
- Vegfa Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001541 ovarian follicle development
- GO:0001569 patterning of blood vessels
- GO:0001701 in utero embryonic development
- GO:0001822 kidney development
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003007 heart morphogenesis
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0040007 growth
- GO:0007498 mesoderm development
- GO:0007595 lactation
- GO:0007610 behavior
- GO:0008083 growth factor activity
- GO:0008201 heparin binding
- GO:0008283 cell proliferation
- GO:0030855 epithelial cell differentiation
- GO:0031077 post-embryonic camera-type eye development
- GO:0042462 eye photoreceptor cell development
- GO:0043117 positive regulation of vascular permeability
- GO:0043129 surfactant homeostasis
- GO:0048010 vascular endothelial growth factor receptor signaling pathway
- GO:0048469 cell maturation
- GO:0048593 camera-type eye morphogenesis
- GO:0048739 cardiac muscle fiber development
- GO:0048844 artery morphogenesis
- GO:0051781 positive regulation of cell division
- GO:0060319 primitive erythrocyte differentiation
- GO:0060749 mammary gland alveolus development
- GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
- Regulatory
- For research purposes only
Applications of VEGF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25ug/ml | 1.0ug/ml | |
| Immunohistology - Paraffin | |||
| Western Blotting | 0.10ug/ml | 0.20ug/ml | |
| ELISA | 0.5ug/ml | ||
| Functional Assays | 1.0ug/ml | 2.0ug/ml | |
| Immunofluorescence | 2.5ug/ml | ||
| Immunohistology - Paraffin 1 | 2.5ug/ml | ||
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used in direct ELISA or as a detection reagent in a sandwich ELISA with AAM51 as a capture reagent and PMP67 as the standard.
- ELISA
- This antibody may be used in indirect ELISA or as a capture reagent in a sandwich ELISA with AAM51B as a detection reagent and PMP67 as the standard.
- Histology Positive Control Tissue
- Kidney
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for VEGF antibody
-
Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
J Exp Med. 204: 3037-47. -
Araújo, W.F. et al. (2015) Renin-angiotensin system (RAS) blockade attenuates growth and metastatic potential of renal cell carcinoma in mice.
Urol Oncol. 33 (9): 389.e1-7. -
Lin, E.Y. et al. (2007) Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages.
Mol Oncol. 1: 288-302. -
HogenEsch H et al. (2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.
Exp Mol Pathol. Oct 26. pii: S0014-4800(16)30084-3. [Epub ahead of print] -
Hogenesch, H. et al. (2016) Angiogenesis in the skin of SHARPIN-deficient mice with chronic proliferative dermatitis.
Exp Mol Pathol. 101 (3): 303-7.
