IFN Gamma antibody
Rabbit anti Human Interferon Gamma
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IFN Gamma
Rabbit anti Human Interferon gamma antibody recognizes human IFN-gamma (Interferon-gamma), a 143 amino acid regulatory cytokine and member of the Type II interferon family. IFN-gamma is involved in the regulation of the immune and inflammatory responses and is secreted primarily by mitogen and antigen activated T lymphocytes and natural killer cells (NK). IFN-gamma is a multifunctional cytokine which signals through the IFN-gamma receptor (IFNGR), found on most immune cells. Properties of IFN-gamma include recruitment of leucocytes to sites of infection, inducement of MHC class I and II antigens, stimulation of anti-microbial and anti-tumour responses of macrophages, Nk cells and neutrophils, and has also been shown to potentiate the antiproliferative effects of IFN-alpha and IFN-beta. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human IFNγ were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IFNγ
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Functional Assays | 0.04 | 0.06ug/ml | |
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- ELISA
- This purified human IFNγ antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IFNγ antibody (AHP775B) as the detection reagent and recombinant human IFNγ (PHP050) as the standard.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IFNγ (PHP050) as the positive control.
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Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Rabbit anti Human Interferon Gamma:Biotin | AHP775B | E WB | 50 µg | Log in | |||
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Description | Rabbit anti Human Interferon Gamma:Biotin | ||||||
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 | ||||||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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Description | TidyBlot Western Blot Detection Reagent:HRP |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Recombinant Human Interferon Gamma | PHP050 | E FN WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Recombinant Human Interferon Gamma | ||||||
Recombinant Human Interferon Gamma | PHP050A | E FN WB | 20 µg | Log in | |||
List Price | Your Price | ||||||
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Description | Recombinant Human Interferon Gamma |
Further Reading
-
Rinderknecht, E. et al. (1984) Natural human interferon-gamma. Complete amino acid sequence and determination of sites of glycosylation.
J Biol Chem. 259 (11): 6790-7. -
Goldberg, M. et al. (1989) Regulation of macrophage growth and antiviral activity by interferon-gamma.
J Cell Biol. 109 (3): 1331-40.
- Synonyms
- Interferon Gamma
- RRID
- AB_609669
- UniProt
- P01579
- Entrez Gene
- IFNG
- GO Terms
- GO:0000060 protein import into nucleus, translocation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0007050 cell cycle arrest
- View More GO Terms
- GO:0050796 regulation of insulin secretion
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032747 positive regulation of interleukin-23 production
- GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
- GO:0034393 positive regulation of smooth muscle cell apoptosis
- GO:0042508 tyrosine phosphorylation of Stat1 protein
- GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0048662 negative regulation of smooth muscle cell proliferation
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051712 positive regulation of killing of cells of other organism
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
- GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
- GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
- GO:0060557 positive regulation of vitamin D biosynthetic process
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
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