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IFN Gamma antibody

Rabbit anti Human Interferon Gamma:Biotin

Product Type
Polyclonal Antibody
Polyclonal IgG
IFN Gamma

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 50 µg loader
List Price Your Price

Rabbit anti Human Interferon gamma antibody recognizes human IFN-gamma (Interferon-gamma), a 143 amino acid regulatory cytokine and member of the Type II interferon family. IFN-gamma is involved in the regulation of the immune and inflammatory responses and is secreted primarily by mitogen and antigen activated T lymphocytes and natural killer cells (NK).

IFN-gamma is a multifunctional cytokine which signals through the IFN-gamma receptor (IFNGR), found on most immune cells. Properties of IFN-gamma include recruitment of leucocytes to sites of infection, inducement of MHC class I and II antigens, stimulation of anti-microbial and anti-tumour responses of macrophages, Nk cells and neutrophils, and has also been shown to potentiate the antiproliferative effects of IFN-alpha and IFN-beta.

Target Species
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human IFNγ were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
For research purposes only
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This biotinylated human IFNγ antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IFNγ antibody (AHP775) as the capture reagent and recombinant human IFNγ (PHP050) as the standard.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IFNγ (PHP050) as the positive control.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Human Interferon Gamma AHP775 E FN P * WB 0.1 mg loader
List Price Your Price
Description Rabbit anti Human Interferon Gamma

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human Interferon Gamma PHP050 E FN WB 0.1 mg
List Price Your Price
Description Recombinant Human Interferon Gamma
Recombinant Human Interferon Gamma PHP050A E FN WB 20 µg
List Price Your Price
Description Recombinant Human Interferon Gamma

Further Reading

  1. Rinderknecht, E. et al. (1984) Natural human interferon-gamma. Complete amino acid sequence and determination of sites of glycosylation.
    J Biol Chem. 259 (11): 6790-7.
  2. Goldberg, M. et al. (1989) Regulation of macrophage growth and antiviral activity by interferon-gamma.
    J Cell Biol. 109 (3): 1331-40.

Interferon Gamma
Entrez Gene
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0050796 regulation of insulin secretion
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032735 positive regulation of interleukin-12 production
GO:0032747 positive regulation of interleukin-23 production
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042508 tyrosine phosphorylation of Stat1 protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051712 positive regulation of killing of cells of other organism
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation


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