IL-4 antibody | CC314
Biotinylated Mouse anti Bovine interleukin-4 antibody, clone CC314 (MCA2372B) used as a detection reagent for the evaluation of IL-4 secretion by Lymph dendritic cells in vitro by ELISA in combination with Mouse anti Bovine interleukin-4, clone CC313 (MCA2371) as a capture reagent.
Image caption:
CD1b+ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b+ L-DCs were incubated at different ratios with 105 allogeneic CD4+ T cells (A) or CD62L+ T cells (C), and tested for proliferation by [3H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).
From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Filter by Application:
E ResetMouse anti Bovine Interleukin-4:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- CC314
- Isotype
- IgG2b
- Specificity
- IL-4
Antibody Quality and Performance Guarantee
| Mouse anti Bovine interleukin-4 antibody, clone CC314 recognizes bovine intetleukin-4 (IL-4), also known as B-cell stimulatory factor 1. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussler et al. 1992). IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC314 has been used successfully as a detection antibody in a sandwich ELISA with Mouse anti Bovine IL-4 antibody, clone CC313 (MCA2371) as a coating reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Recombinant bovine IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells form immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1ug/ml | 5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- ELISA
- Biotin conjugated Mouse anti Bovine interleukin-4 antibody, clone CC314 may be used as detection reagent in sandwich ELISA assays for bovine IL-4 with MCA2371 as capture reagent, and recombinant bovine IL-4 (PBP006) as a standard.
References for IL-4 antibody
-
Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.
J Immunol Methods. 301 (1-2): 114-23. -
Abbott JR et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
J Immunol. 174 (11): 6702-15. -
Stabel JR et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.
Clin Vaccine Immunol. 20 (6): 848-57. -
Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine.
Clin Vaccine Immunol. 18: 2079-89. -
Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.
Res Vet Sci. 93: 662-7. -
Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
PLoS One. 7: e30430. -
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print] -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7.
View The Latest Product References
-
Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
Vet Immunol Immunopathol. 237: 110268.
- RRID
- AB_2127032
- UniProt
- P30367
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0042113 B cell activation
- GO:0043066 negative regulation of apoptosis
- GO:0045348 positive regulation of MHC class II biosynthetic process
- View More GO Terms
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
MCA2372B
0812
158340
1804
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