IL-4 antibody | CC314

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Anti Bovine Interleukin-4 Antibody, clone CC314 gallery image 1 — **Published customer image:**
Biotinylated Mouse anti Bovine interleukin-4 antibody, clone CC314 (**MCA2372B**) used as a detection reagent for the evaluation of IL-4 secretion by Lymph dendritic cells *in vitro* by ELISA in combination with Mouse anti Bovine interleukin-4, clone CC313 (MCA2371) as a capture reagent.
**Image caption:**
CD1b⁺ L-DCs are efficient APC for the priming of allogeneic naive T cells. Sorted CD1b⁺ L-DCs were incubated at different ratios with 10⁵ allogeneic CD4⁺ T cells (A) or CD62L⁺ T cells (C), and tested for proliferation by [³H]-thymidine incorporation after six days of culture. Data express the median (quartiles) of counts per minute (cpm) from triplicates for each condition. After three days of culture, supernatants were sampled and IFN-γ (black bars) and IL-4 (grey bars) production analysed by ELISA (B, D). The results shown are from one representative experiment out of four performed on different sheep (A) or out of two performed on one sheep(C).

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b⁺ Lymph Dendritic Cells to Present *Salmonella* Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine Interleukin-4:Biotin

Product Type: Monoclonal Antibody
Clone: CC314
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2372B	E			0.25 mg

Mouse anti Bovine interleukin-4 antibody, clone CC314 recognizes bovine intetleukin-4 (IL-4), also known as B-cell stimulatory factor 1. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussler et al. 1992).

IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.

Mouse anti Bovine interleukin-4, clone CC314 has been used successfully as a detection antibody in a sandwich ELISA with Mouse anti Bovine IL-4 antibody, clone CC313 (MCA2371) as a coating reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Biotin - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

Recombinant bovine IL-4.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells form immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P30367 Related reagents
Entrez Gene: IL4 Related reagents
GO Terms: GO:0005125 cytokine activity; GO:0005136 interleukin-4 receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0030890 positive regulation of B cell proliferation; GO:0042113 B cell activation; GO:0043066 negative regulation of apoptosis; GO:0045348 positive regulation of MHC class II biosynthetic process; GO:0045671 negative regulation of osteoclast differentiation; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048295 positive regulation of isotype switching to IgE isotypes; GO:0048304 positive regulation of isotype switching to IgG isotypes
Regulatory: For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1ug/ml	5ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
ELISA: This biotin conjugate may be used as detection reagent in sandwich ELISA assays for bovine IL-4 with MCA2371 as capture reagent, and recombinant bovine IL-4 (PBP006) as a standard.

Application Based External Images

ELISA

Product Specific References

References for IL-4 antibody

Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4.
J Immunol Methods. 301 (1-2): 114-23.
Abbott JR et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
J Immunol. 174 (11): 6702-15.
Stabel JR et al. (2013) Disparate Host Immunity to Mycobacterium avium subsp. paratuberculosis Antigens in Calves Inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii and M. bovis.
Clin Vaccine Immunol. 20 (6): 848-57.
Stabel, J.R. et al. (2011) Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine.
Clin Vaccine Immunol. 18: 2079-89.
Marinaro, M. et al. (2012) Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats.
Res Vet Sci. 93: 662-7.
Olivier, M. et al. (2012) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
PLoS One. 7: e30430.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]

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