VEGF antibody
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Rabbit anti Rat VEGF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- VEGF
Antibody Quality and Performance Guarantee
| Rabbit anti Rat VEGF antibody specifically recognizes rat VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which acts as a vasodilator, regulator of vasculogenesis, and is a potent stimulator of angiogenesis of both normal and cancerous cells. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), and the functional forms of VEGF, which exist as disulphide-linked homodimers, are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. |
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to rat VEGF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat VEGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
0.05 | 0.1ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Purified rabbit anti rat VEGF may be used in an indirect ELISA or as the capture reagent in a sandwiche ELISA with our biotinylated VEGF antibody as the detection reagent.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- Synonyms
- VPF
- RRID
- AB_2212685
- UniProt
- P16612
- Entrez Gene
- Vegfa
- GO Terms
- GO:0001974 blood vessel remodeling
- GO:0016020 membrane
- GO:0005604 basement membrane
- GO:0005615 extracellular space
- GO:0008083 growth factor activity
- GO:0008201 heparin binding
- GO:0009409 response to cold
- GO:0042088 T-helper 1 type immune response
- GO:0042803 protein homodimerization activity
- View More GO Terms
- GO:0043117 positive regulation of vascular permeability
- GO:0045779 negative regulation of bone resorption
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048255 mRNA stabilization
- GO:0048286 lung alveolus development
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0051781 positive regulation of cell division
AAR42
145582
159852
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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