RANTES antibody


RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant Human RANTES
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P13501
- Entrez Gene
- CCL5
- GO Terms
- GO:0009615 response to virus
- GO:0007155 cell adhesion
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006874 cellular calcium ion homeostasis
- GO:0006887 exocytosis
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0006979 response to oxidative stress
- GO:0007267 cell-cell signaling
- GO:0008009 chemokine activity
- GO:0042056 chemoattractant activity
- GO:0045071 negative regulation of viral genome replication
- Regulatory
- For research purposes only
Applications of RANTES antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.25 | 1.0ug/ml | |
Immunohistology - Paraffin | |||
Western Blotting | 0.1 | 0.2ug/ml | |
ELISA | 0.5 ug/ml | 2.0ug/ml | |
Functional Assays | 5.0ug/ml | 7.0ug/ml | |
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.1ug/ml | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- ELISA
- This biotinylated human RANTES antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human RANTES antibody (AHP340G) as the capture reagent and recombinant human RANTES (PHP062) as the standard.
- ELISA
- This purified human RANTES antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human RANTES antibody (AHP340B) as the detection reagent and recombinant human RANTES (PHP062) as the standard.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human RANTES (PHP062) as the positive control.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human RANTES (PHP062) as the positive control.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for RANTES antibody
-
Appay, V. et al. (2000) RANTES activates antigen-specific cytotoxic T lymphocytes in a mitogen-like manner through cell surface aggregation.
Int Immunol. 12 (8): 1173-82. -
Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
Nature. 381 (6584): 667-73. -
Cheng, H. et al. (2016) TWEAK/Fn14 activation induces keratinocyte proliferation under psoriatic inflammation.
Exp Dermatol. 25 (1): 32-7.
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