RANTES antibody

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Anti Human RANTES Antibody gallery image 1 — Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human RANTES (**AHP340G**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Anti Human RANTES Antibody gallery image 2 — Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human RANTES (**AHP340G**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Rabbit anti Human RANTES:Biotin

Rabbit anti Human RANTES

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP340B	E P WB			50 µg
AHP340G	E FN P* WB			0.1 mg

Rabbit anti Human RANTES antibody recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells.

RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Biotin - lyophilised
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution: Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation: Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Preservative Stabilisers: None present
Immunogen: Recombinant Human RANTES
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml after reconstitution.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at -20^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 6 months from date of reconstitution.
Shelf Life: 3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt: P13501 Related reagents
Entrez Gene: CCL5 Related reagents
GO Terms: GO:0009615 response to virus; GO:0007155 cell adhesion; GO:0004871 signal transducer activity; GO:0005615 extracellular space; GO:0006874 cellular calcium ion homeostasis; GO:0006887 exocytosis; GO:0006955 immune response; GO:0006968 cellular defense response; GO:0006979 response to oxidative stress; GO:0007267 cell-cell signaling; GO:0008009 chemokine activity; GO:0042056 chemoattractant activity; GO:0045071 negative regulation of viral genome replication
Regulatory: For research purposes only

Applications of RANTES antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.25	1.0ug/ml
Immunohistology - Paraffin
Western Blotting	0.1	0.2ug/ml
ELISA	0.5 ug/ml	2.0ug/ml
Functional Assays	5.0ug/ml	7.0ug/ml
Immunohistology - Paraffin ¹
Western Blotting	0.1ug/ml	0.2ug/ml

¹ This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: This biotinylated human RANTES antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human RANTES antibody (AHP340G) as the capture reagent and recombinant human RANTES (PHP062) as the standard.
ELISA: This purified human RANTES antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human RANTES antibody (AHP340B) as the detection reagent and recombinant human RANTES (PHP062) as the standard.
Western Blotting: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human RANTES (PHP062) as the positive control.
Western Blotting: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human RANTES (PHP062) as the positive control.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

Product Specific References

References for RANTES antibody

Appay, V. et al. (2000) RANTES activates antigen-specific cytotoxic T lymphocytes in a mitogen-like manner through cell surface aggregation.
Int Immunol. 12 (8): 1173-82.
Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
Nature. 381 (6584): 667-73.
Cheng, H. et al. (2016) TWEAK/Fn14 activation induces keratinocyte proliferation under psoriatic inflammation.
Exp Dermatol. 25 (1): 32-7.

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