RANTES antibody

Rabbit anti Human RANTES

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RANTES

Product Code Applications Pack Size List Price Your Price Qty
AHP340G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN P * WB 0.1 mg loader
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Rabbit anti Human RANTES antibody recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells.

RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 ug/ml 2.0ug/ml
Functional Assays 5.0ug/ml 7.0ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1ug/ml 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This purified human RANTES antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human RANTES antibody (AHP340B) as the detection reagent and recombinant human RANTES (PHP062) as the standard.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human RANTES (PHP062) as the positive control.

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Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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References for RANTES antibody

  1. Appay, V. et al. (2000) RANTES activates antigen-specific cytotoxic T lymphocytes in a mitogen-like manner through cell surface aggregation.
    Int Immunol. 12 (8): 1173-82.
  2. Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
    Nature. 381 (6584): 667-73.
  3. Cheng, H. et al. (2016) TWEAK/Fn14 activation induces keratinocyte proliferation under psoriatic inflammation.
    Exp Dermatol. 25 (1): 32-7.

Synonyms
CCL5
RRID
AB_2228621
UniProt
P13501
Entrez Gene
CCL5
GO Terms
GO:0009615 response to virus
GO:0007155 cell adhesion
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006887 exocytosis
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0006979 response to oxidative stress
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0042056 chemoattractant activity
GO:0045071 negative regulation of viral genome replication
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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