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IL-1 Beta antibody

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Anti Rat Interleukin-1 beta Antibody thumbnail image 1 Anti Rat Interleukin-1 beta Antibody thumbnail image 2 Anti Rat Interleukin-1 beta Antibody thumbnail image 3 Anti Rat Interleukin-1 beta Antibody thumbnail image 4
Anti Rat Interleukin-1 beta Antibody gallery image 1
Published customer image:
Rabbit anti Rat Interleukin-1β antibody used for the detection of IL-1β expressing neurons in rat brain by immnofluorescence and immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Neuronal expression of IL-1β in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1β (A) and TNF-α (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1β + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing IL-1β. (E) Immunohistochemical staining of IL-1β expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50μm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *P ≤ 0.05, **P ≤ 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein.

From: Savard A, Lavoie K, Brochu ME, Grbic D, Lepage M, Gris D, Sebire G.
Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.
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This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat Interleukin-1 beta Antibody gallery image 2
Published customer image:
Rabbit anti Rat interleukin-1 beta antibody (AAR15) used to label interleukin-1β by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Immunohistochemical expression pattern of cell positivity for IL-1β in the experimental groups treated with oral administration of vehicle only (Vehicle), formononetin at 10 mg/kg (FNT), and ibuprofen at 10 mg/kg (LSAB, 400×). (B) Assessment of the mean number of IL-1β-positive cells for histological field (0.025 mm2). Differences in comparison with Vehicle are expressed as ** p < 0.01, whereas differences in comparison with Sham are expressed as ## p < 0.011 (ANOVA and Bonferroni’s multiplex comparison test).

From: Barreto, R.B.; Santana, B.H.d.; Martins, B.M.; Porto, E.S.; Severino, P.; Cardoso, J.C.; Souto, E.B.; Albuquerque-Júnior, R.L.C.d.
Application of Formononetin for the Treatment of Knee Osteoarthritis Induced by Medial Meniscectomy in a Rodent Model.
Appl. Sci. 2022, 12, 8591.
doi: 10.3390/app12178591.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat Interleukin-1 beta Antibody gallery image 3
Published customer image:
Rabbit anti Rat Interleukin-1 beta antibody (AAR15) used to assess IL-1β; espression levels in spontaneously hypertensive rats by western blotting.
Image caption:
Modulation of inflammatory markers. Lysates of the heart from normotensive Wistar Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and SHR treated with (+)-thioctic acid lysine salt [SHR (+)-TIO] were immunoblotted with specific antibodies against (A) interleukin 1 beta (IL-1 beta) Graphs values indicate the ratio of densitometric analysis of bands to β-actin levels used as the reference loading control. Data are mean ±S.D. *= p <0.05 vs. WKY; #=p <0.05 vs. SHR. Blots are representative of each experimental group.

From: Roy P, Tomassoni D, Martinelli I, Bellitto V, Nittari G, Amenta F, Tayebati SK.
Protective effects of the R-(+)-thioctic acid treatment: possible anti-inflammatory activity on heart of hypertensive rats.
BMC Complement Med Ther. 2024 Jul 24;24(1):281.
doi: 10.1186/s12906-024-04547-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat Interleukin-1 beta Antibody gallery image 4
Published customer image:
Rabbit anti Rat Interleukin-1 beta antibody (AAR15) used to assess IL-1β; espression levels in spontaneously hypertensive rats byimmunofluorescence.
Image caption:
Up-regulation of inflammatory cytokines. Sections of the heart of normotensive Wistar Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and SHR treated with thioctic acid lysine salt [SHR (+)-TIO] processed for the confocal immunofluorescence of (A) interleukin-1 beta (IL-1 beta). The graphs showed the values of mean fluorescence intensity (MFI). The immunoreactive cardiomyocytes are indicated with the arrowheads. Data are mean ±S.D. *= p <0.05 vs. WKY; #=p <0.05 vs. SHR. Magnification 40×, zoom 2. Scale bar: 10 μm. NC, negative control.

From: Roy P, Tomassoni D, Martinelli I, Bellitto V, Nittari G, Amenta F, Tayebati SK.
Protective effects of the R-(+)-thioctic acid treatment: possible anti-inflammatory activity on heart of hypertensive rats.
BMC Complement Med Ther. 2024 Jul 24;24(1):281.
doi: 10.1186/s12906-024-04547-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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IF P WB

Rabbit anti Rat Interleukin-1 beta:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-1 Beta

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AAR15B
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Rabbit anti Rat Interleukin-1 beta antibody recognizes rat IL-1 beta. Interleukin-1β is a 152 amino acid active pro-inflammatory cytokine produced with an additional 116 amino acid pro-peptide region. IL-1&beta has a broad mode of action, stimulating prostaglandin synthesis, neutrophil, T cell and B cell activation and collagen synthesis.

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to rat IL-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Carrier Free
Yes
Immunogen
Recombinant rat IL-1 beta (PRP23).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with AAR15G as the capture reagent and PRP23 as the standard.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IL-1 Beta antibody

  1. Girard, S. (2008) Pro-inflammatory disequilibrium of the IL-1 beta/IL-1ra ratio in an experimental model of perinatal brain damages induced by lipopolysaccharide and hypoxia-ischemia.
    Cytokine. 43: 54-62.
  2. Weksler-Zangen, S. et al. (2008) Impaired glucose-stimulated insulin secretion is coupled with exocrine pancreatic lesions in the Cohen diabetic rat.
    Diabetes. 57: 279-87.
  3. Glatz, T. et al. (2010) Peroxisome-proliferator-activated receptors gamma and peroxisome-proliferator-activated receptors beta/delta and the regulation of interleukin 1 receptor antagonist expression by pioglitazone in ischaemic brain.
    J Hypertens. 28: 1488-97.
  4. Calveley, V.L. et al. (2010) Genistein can mitigate the effect of radiation on rat lung tissue.
    Radiat Res. 173 (5): 602-11.
  5. Mahmood, J. et al. (2011) Mitigation of radiation-induced lung injury by genistein and EUK-207.
    Int J Radiat Biol. 87: 889-901.
  6. Mahmood, J. et al. (2013) Mitigation of radiation-induced lung injury with EUK-207 and genistein: effects in adolescent rats.
    Radiat Res. 179 (2): 125-34.
  7. Cho, G.S. et al. (2013) N-Methyl-D-aspartate receptor antagonists memantine and MK-801 attenuate the cerebral infarct accelerated by intracorpus callosum injection of lipopolysaccharides.
    Neurosci Lett. 538: 9-14.
  8. Savard, A. et al. (2013) Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy.
    J Neuroinflammation. 10: 110.
    9. View The Latest Product References
  9. Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
    Diabetes. 64 (5): 1780-5.
  10. Bergeron, J. et al. (2016) Activation of the IL-1β/CXCL1/MMP-10 axis in chorioamnionitis induced by inactivated Group B Streptococcus.
    Placenta. 47: 116-23.
  11. Alizadeh, A. et al. (2017) Neuregulin-1 positively modulates glial response and improves neurological recovery following traumatic spinal cord injury.
    Glia. 65 (7): 1152-75.
  12. Miyai, H. et al. (2017) Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats.
    Arch Oral Biol. 82: 247-55.
  13. Kataria, H. et al. (2018) Neuregulin-1 promotes remyelination and fosters a pro-regenerative inflammatory response in focal demyelinating lesions of the spinal cord.
    Glia. 66 (3): 538-61.
  14. Dyck, S. et al. (2018) Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
    J Neuroinflammation. 15 (1): 90.
  15. Barreto, R.B. et al. (2022) Application of Formononetin for the Treatment of Knee Osteoarthritis Induced by Medial Meniscectomy in a Rodent Model
    Applied Sciences. 12 (17): 8591.
  16. Hart, C.G. et al. (2020) Acute upregulation of bone morphogenetic protein-4 regulates endogenous cell response and promotes cell death in spinal cord injury.
    Exp Neurol. 325: 113163.
  17. Roy, P. et al. (2024) Protective effects of the R-(+)-thioctic acid treatment: possible anti-inflammatory activity on heart of hypertensive rats.
    BMC Complement Med Ther. 24 (1): 281.

Immunohistology - Paraffin

RRID
AB_2233647
UniProt
Q63264
Entrez Gene
Il1b
GO Terms
GO:0001660 fever generation
GO:0001666 response to hypoxia
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007568 aging
GO:0007613 memory
GO:0042493 response to drug
GO:0009408 response to heat
GO:0010193 response to ozone
GO:0010243 response to organic nitrogen
GO:0010332 response to gamma radiation
GO:0014050 negative regulation of glutamate secretion
GO:0019221 cytokine-mediated signaling pathway
GO:0030728 ovulation
GO:0032355 response to estradiol stimulus
GO:0032496 response to lipopolysaccharide
GO:0032755 positive regulation of interleukin-6 production
GO:0032874 positive regulation of stress-activated MAPK cascade
GO:0033280 response to vitamin D
GO:0033591 response to L-ascorbic acid
GO:0035176 social behavior
GO:0042060 wound healing
GO:0043065 positive regulation of apoptosis
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043278 response to morphine
GO:0043507 positive regulation of JUN kinase activity
GO:0046330 positive regulation of JNK cascade
GO:0051384 response to glucocorticoid stimulus
GO:0051781 positive regulation of cell division
GO:0070374 positive regulation of ERK1 and ERK2 cascade

AAR15B

160401 160823 166532

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