Granzyme B antibody | GB11
R-phycoerythrin conjugated Mouse anti Human granzyme B antibody, clone GB11 (MCA2120PE) used to assess expression levels of granzyme B in peripheral blood mononuclear cells by flow cyrometry.
Image caption:
The percentage of granzyme B-positive cells in CD8+lymphocytes stimulated with allogenic cells upon exposure to CB with IL-2. PBMCs were harvested from the three groups, representing allostimulation, CB-exposed allostimulation, and CB-exposed allostimulation with IL-2, and assayed for the percentage of cells positive for intracellular granzyme B using FCM. (a) Representative histograms of intracellular granzyme B in CD8+lymphocytes. A nonstained control (gray) is shown in each panel. (b) Cumulative data showing the ratio of each group to the allostimulation control was calculated and compared among the groups. Data represent the mean +SD from four independent experiments using PBMCs. Significant differences are indicated by an asterisk (∗ p <0.05). No significant difference (N.S.) is also indicated. (+), the culture with allogenic PBMCs without CB; (+)/CB, the culture with allogenic PBMCs with CB; (+)/CB/IL-2, the culture with allogenic PBMCs with CB and IL-2.
From: Kumagai-Takei N, Nishimura Y, Matsuzaki H, Lee S, Yoshitome K, Hayashi H, Otsuki T.
The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency.
J Immunol Res. 2016;2016:7484872.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Granzyme B
- Product Type
- Monoclonal Antibody
- Clone
- GB11
- Isotype
- IgG1
- Specificity
- Granzyme B
Antibody Quality and Performance Guarantee
| Mouse anti Granzyme B antibody, clone GB11 recognizes the serine protease Granzyme B, important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. Mouse anti Granzyme B antibody, clone GB11 is suitable for the detection of Granzyme B expressing cells by flow cytometry. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Tris buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Purified human Granzyme B.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2ug/ml | 5ug/ml |
| Flow Cytometry 1 | |
1/10 | 1/100 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
- ELISA
- Mouse anti Human granzyme B antibody, clone GB11 may be used as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (MCA2119B) as a detection reagent.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Hsi, B.L. & Yeh, C.J. (1986) Monoclonal antibodies to human amnion.
J Reprod Immunol. 9 (1): 11-21.
References for Granzyme B antibody
-
Spaeny-Dekking, E.H. et al. (1998) Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo.
J Immunol. 160 (7): 3610-6. -
Wever, D. C. et al. (1998) The CD8+ Granzyme B+ T cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells.
Immunol. 93: 383 -
Mahrus, S. & Craik, C.S. (2005) Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells.
Chem Biol. 12: 567-77. -
Tschopp, C.M. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma.
Blood.108:2290-9. -
Schleypen, J.S. et al. (2006) Cytotoxic markers and frequency predict functional capacity of natural killer cells infiltrating renal cell carcinoma.
Clin Cancer Res. 12: 718-25. -
Hallermalm, K. et al. (2008) Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes.
J Immunol. 180: 3766-74. -
Clayton, A. et al. (2008) Human tumor-derived exosomes down-modulate NKG2D expression.
J Immunol. 180: 7249-58. -
Hufner, K. et al. (2009) Fewer latent herpes simplex virus type 1 and cytotoxic T cells occur in the ophthalmic division than in the maxillary and mandibular divisions of the human trigeminal ganglion and nerve.
J Virol. 83: 3696-703.
View The Latest Product References
-
Kumar, D. et al. (2009) JNK MAPK pathway regulates constitutive transcription of CCL5 by human NK cells through SP1.
J Immunol. 182: 1011-20. -
Davis, C.C. et al. (2010) Interleukin-7 permits Th1/Tc1 maturation and promotes ex vivo expansion of cord blood T cells: a critical step toward adoptive immunotherapy after cord blood transplantation.
Cancer Res. 70: 5249-58. -
Hodge, S. et al. (2015) Increased CD8 T-cell granzyme B in COPD is suppressed by treatment with low-dose azithromycin.
Respirology. 20 (1): 95-100. -
Kumagai-Takei, N. et al. (2016) The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency.
J Immunol Res. 2016: 7484872. -
Kumagai-Takei, N. et al. (2021) Effect of IL-15 addition on asbestos-induced suppression of human cytotoxic T lymphocyte induction.
Environ Health Prev Med. 26 (1): 50. -
Jimenez, O. et al. (2019) M1-like macrophage polarization prevails in young children with classic Hodgkin Lymphoma from Argentina.
Sci Rep. 9 (1): 12687. -
El-Garawani, I. et al. (2021) Genotoxicity linked to occupational exposure in uranium mine workers: Granzyme B and apoptotic changes.
Environ Sci Pollut Res Int. 28 (27): 36793-802.
- RRID
- AB_2114582
- UniProt
- P10144
- Entrez Gene
- GZMB
- GO Terms
- GO:0005515 protein binding
- GO:0001772 immunological synapse
- GO:0004252 serine-type endopeptidase activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006922 cleavage of lamin
- GO:0019835 cytolysis
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- View More GO Terms
View more products with GRANZYME B specificity
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