CTGF antibody

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Rabbit anti Human CTGF:Biotin

Rabbit anti Human CTGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1278B E P WB 50 µg
AHP1278 E FN IF P* WB 0.1 mg
Rabbit anti Human CTGF antibody recognizes human CTGF (connective tissue growth factor), a 323 amino acid ~38 kDa polypeptide member of the CCN family of secreted cysteine rich regulatory proteins. CTGF is produced by vascular endothelial and umbilical vein cells, and interacts with several other growth factors, including transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs).

CTGF is a heparin-binding pleiotropic growth factor involved in chondrogenesis and potentially skeletogenesis. CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and also promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF induces extracellular matrix formation, including the deposition of excessive collagen in fibrotic diseases, and has also been implicated in wound healing.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human CTGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human CTGF.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P29279 Related reagents
Entrez Gene
CTGF Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0006260 DNA replication
GO:0001558 regulation of cell growth
GO:0005520 insulin-like growth factor binding
GO:0005578 proteinaceous extracellular matrix
GO:0008201 heparin binding
GO:0008544 epidermis development
GO:0009611 response to wounding
GO:0044255 cellular lipid metabolic process
Regulatory
For research purposes only

Applications of CTGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 6.0 9.0ug/ml
Immunofluorescence
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human CTGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CTGF antibody (AHP1278) as the capture reagent and recombinant human CTGF (PHP174) as the standard.
ELISA
This purified human CTGF antibody may ne used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human CTGF antibody (AHP1278B) as the detection reagent and recombinant human CTGF (PHP174) as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunofluorescence

Product Specific References

References for CTGF antibody

  1. Waddell, J.M. et al. (2011) CTGF expression is up-regulated by PROK1 in early pregnancy and influences HTR-8/Svneo cell adhesion and network formation.
    Hum Reprod. 26 (1): 67-75.
  2. Westermann, D. et al. (2009) Gene deletion of the kinin receptor B1 attenuates cardiac inflammation and fibrosis during the development of experimental diabetic cardiomyopathy.
    Diabetes. 58: 1373-81.
  3. Vasilieva, O.V. et al. (2016) Connective tissue growth factor (CTGF) in the human dermis through ontogenesis.
    Russian Journal of Developmental Biology. 47 (2): 63-68.

Further Reading

  1. Moussad, E.E. & Brigstock, D.R. (2000) Connective tissue growth factor: what's in a name?
    Mol Genet Metab. 71 (1-2): 276-92.