CTGF antibody
Rabbit anti HumAN ctgf ANTIBODY (AHP1278) used to evaluate CTGF expression in rat adrenal glands by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CTGF expression in adrenal glands 400× magnified in the control (A), experimental (B) and reversibility control (C) groups. Visible insular and irregular expression. IHC staining (bar = 50μm).
From: Zarobkiewicz MK, Wożniakowski MM, Wawryk-Gawda E, Sławiński MA, Halczuk P, Korolczuk A, Jodłowska-Jędrych B.
Decrease in Lipid Droplets in Adrenal Cortex of Male Wistar Rats after Chronic Exposure to Energy Drinks.
Medicina (Kaunas). 2018 Nov 19;54(5):90.
doi: 10.3390/medicina54050090.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
P ResetRabbit anti Human CTGF
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CTGF
Antibody Quality and Performance Guarantee
| Rabbit anti Human CTGF antibody recognizes human CTGF (connective tissue growth factor), a 323 amino acid ~38 kDa polypeptide member of the CCN family of secreted cysteine rich regulatory proteins. CTGF is produced by vascular endothelial and umbilical vein cells, and interacts with several other growth factors, including transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs). CTGF is a heparin-binding pleiotropic growth factor involved in chondrogenesis and potentially skeletogenesis. CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and also promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF induces extracellular matrix formation, including the deposition of excessive collagen in fibrotic diseases, and has also been implicated in wound healing. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human CTGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human CTGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
6.0 | 9.0ug/ml |
| Immunofluorescence | |
||
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified human CTGF antibody may ne used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human CTGF antibody (AHP1278B) as the detection reagent and recombinant human CTGF (PHP174) as the standard.
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for CTGF antibody
-
Westermann, D. et al. (2009) Gene deletion of the kinin receptor B1 attenuates cardiac inflammation and fibrosis during the development of experimental diabetic cardiomyopathy.
Diabetes. 58: 1373-81. -
Waddell, J.M. et al. (2011) CTGF expression is up-regulated by PROK1 in early pregnancy and influences HTR-8/Svneo cell adhesion and network formation.
Hum Reprod. 26 (1): 67-75. -
Vasilieva. O.V. et al. (2016) Connective tissue growth factor (CTGF) in the human dermis through ontogenesis
Russian Journal of Developmental Biology. 47 (2): 63-68. -
Zarobkiewicz, M.K. et al. (2018) Decrease in Lipid Droplets in Adrenal Cortex of Male Wistar Rats after Chronic Exposure to Energy Drinks.
Medicina (Kaunas). 54 (5):90.
Further Reading
-
Moussad, E.E. & Brigstock, D.R. (2000) Connective tissue growth factor: what's in a name?
Mol Genet Metab. 71 (1-2): 276-92.
- Synonyms
- Connective Tissue Growth Factor
- RRID
- AB_2087370
- UniProt
- P29279
- Entrez Gene
- CTGF
- GO Terms
- GO:0005886 plasma membrane
- GO:0006260 DNA replication
- GO:0001558 regulation of cell growth
- GO:0005520 insulin-like growth factor binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0008201 heparin binding
- GO:0008544 epidermis development
- GO:0009611 response to wounding
- GO:0044255 cellular lipid metabolic process
- View More GO Terms
AHP1278
151215
155862
164604
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