IL-10 antibody | CC320

Mouse anti Bovine Interleukin-10:Biotin

Product Type
Monoclonal Antibody

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Mouse anti Bovine Interleukin-10 antibody, clone CC320 recognizes bovine IL-10.

Mouse anti Bovine Interleukin-10 antibody, clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Plasmid cDNA encoding bovine IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2ug/ml 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Biotin conjugated Mouse anti Bovine interleukin-10 antibody, clone CC320 may be used as detection reagent in a sandwich ELISA assay for bovine IL-10 with MCA2110 as capture reagent. (Bannermann et al. 2004).

References for IL-10 antibody

  1. Hoek, A. et al. (2009) Subpopulations of bovine WC1(+) gammadelta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo.
    Vet Res. 40:6.
  2. Denis, M. et al. (2007) Enhancement of the sensitivity of the whole-blood gamma interferon assay for diagnosis of Mycobacterium bovis infections in cattle.
    Clin Vaccine Immunol. 14: 1483-9.
  3. Flynn, R.J. and Mulcahy, G. (2008) Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages.
    Infect Immun. 76: 678-84.
  4. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
    J Immunol. 174: 6702-15.
  5. Bannerman, D.D. et al. (2004) Characterization of the bovine innate immune response to intramammary infection with Klebsiella pneumoniae.
    J Dairy Sci. 87: 2420-32.
  6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  7. Berger, S.T. and Griffin, F.T. (2006) A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
    Immunol Cell Biol. 84: 349-56.
  8. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
    Vet Res. 41: 14.
  9. View The Latest Product References
  10. Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.
    Biol Reprod. 77: 914-22.
  11. Dean, G.S. et al. (2008) Isoniazid treatment of Mycobacterium bovis in cattle as a model for human tuberculosis.
    Tuberculosis (Edinb). 88: 586-94.
  12. Guan, G. et al. (2010) Course of infection by Babesia sp. BQ1 (Lintan) and B. divergens in sheep depends on the production of IFNgamma and IL10.
    Parasite Immunol. 32: 143-52.
  13. Karcher, E.L. et al. (2008) Osteopontin: a novel cytokine involved in the regulation of Mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle.
    J Dairy Sci. 91: 3079-91.
  14. Lei, L. and Hostetter, J.M. (2007) Limited phenotypic and functional maturation of bovine monocyte-derived dendritic cells following Mycobacterium avium subspecies paratuberculosis infection in vitro.
    Vet Immunol Immunopathol. 120: 177-86.
  15. Parker, D.G. et al. (2009) A steroid-inducible promoter for the cornea.
    Br J Ophthalmol. 93: 1255-9.
  16. Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
    J Immunol. 180: 5963-72.
  17. Souza, C.D. et al. (2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    Microbes Infect. 9: 1545-52.
  18. Stabel, J.R. and Robbe-Austerman, S. (2011) Early immune markers associated with Mycobacterium avium subsp. paratuberculosis infection in a neonatal calf model.
    Clin Vaccine Immunol. 18: 393-405.
  19. Van Rhijn, I. et al. (2007) Massive, sustained gammadelta T cell migration from the bovine skin in vivo.
    J Leukoc Biol. 81: 968-73.
  20. Yamaguchi, T. et al. (2010) Generation of IFN-gamma-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines.
    Vet Parasitol. 171: 207-15.
  21. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
    Vet Immunol Immunopathol. 85 (3-4): 213-23.
  22. Buza JJ et al. (2004) Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium avium subsp. paratuberculosis in experimentally infected cattle with paratuberculosis.
    Infect Immun. 72 (4): 2425-8.
  23. Scandurra, G.M. et al. (2010) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
    Infect Immun. 78 (3): 1383-9.
  24. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83 (1): 48-55.
  25. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
    Int Arch Allergy Immunol. 144: 325-37.
  26. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  27. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  28. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
    Clin Experiment Ophthalmol. 38: 405-13.
  29. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  30. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
    Clin Vaccine Immunol. 16 (7): 1003-11.
  31. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  32. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
    J Virol. 82: 1777-86.
  33. Ma&347;lanka T et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
    Pol J Vet Sci. 15 (1): 11-20.
  34. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
    J Comp Pathol. 148: 157-72.
  35. Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
    PLoS One. 10 (5): e0124144.
  36. Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.
    PLoS One. 11 (4): e0154172.
  37. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
    Infect Immun. 85 (2): e00845-16.
  38. Pomeroy, B. et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.
    Vet Immunol Immunopathol. 182: 37-42.
  39. Pomeroy, B. et al. (2015) Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion.
    Vet Immunol Immunopathol. 167 (1-2): 22-9.
  40. Davidson, J.O. et al. (2021) Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep.
    Stem Cells Transl Med. 10 (3): 427-40.
  41. Borghesi, J. et al. (2020) Effects of doxorubicin associated with amniotic membrane stem cells in the treatment of canine inflammatory breast carcinoma (IPC-366) cells.
    BMC Vet Res. 16 (1): 353.
  42. Galinsky, R. et al. (2020) Tumor necrosis factor inhibition attenuates white matter gliosis after systemic inflammation in preterm fetal sheep.
    J Neuroinflammation. 17 (1): 92.
  43. Westover, A. et al. (2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
    Front Physiol. 8: 871.
  44. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-7.
  45. Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
    Vet Immunol Immunopathol. 211: 44-8.
  46. Stabel, J.R. & Bannantine, J.P. (2019) Divergent Antigen-Specific Cellular Immune Responses during Asymptomatic Subclinical and Clinical States of Disease in Cows Naturally Infected with Mycobacterium avium. subsp. paratuberculosis.
    Infect Immun. 88(1):e00650-19.
  47. Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii.i and M. bovis..
    Vet Immunol Immunopathol. 237: 110268.
  48. Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
    Front Vet Sci. 7: 9.
  49. Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model.
    J Dairy Sci. 105 (5): 4335-53.
  50. Santillo, A. et al. (2022) Feeding tannins to dairy cows in different seasons improves the oxidative status of blood plasma and the antioxidant capacity of cheese.
    J Dairy Sci. 105 (11): 8609-20.



Flow Cytometry

Functional Assays

Entrez Gene
GO Terms
GO:0002237 response to molecule of bacterial origin
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042095 interferon-gamma biosynthetic process
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus


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