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IL-10 antibody | CC320

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Anti Bovine Interleukin-10 Antibody, clone CC320 thumbnail image 1 Anti Bovine Interleukin-10 Antibody, clone CC320 thumbnail image 2 Anti Bovine Interleukin-10 Antibody, clone CC320 thumbnail image 3 Anti Bovine Interleukin-10 Antibody, clone CC320 thumbnail image 4 Anti Bovine Interleukin-10 Antibody, clone CC320 thumbnail image 5
Anti Bovine Interleukin-10 Antibody, clone CC320 gallery image 1
Published customer image:
Biotinylated Mouse anti Bovine interleukin-10 antibody, clone CC320 (MCA2111B) used as a detection reagent for the determination of IL-10 levels in ovine plasma by ELISA
Image caption:
Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p ≤ 0.0001.

From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. (2015)
Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.
PLoS ONE 10(5): e0124144.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine Interleukin-10 Antibody, clone CC320 gallery image 2
Published customer image:
Biotinylated Mouse anti Bovine IL-10 antibody, clone CC320 (MCA2111B) used to evaluate IL-10 expression by canine carcinoma cells by flow cytometry.
Image caption:
Cell marker expression (% of cells) by flow cytometry of control and cells treated with doxorubicin (DOXO, 10 µg/mL); amniotic membrane stem cells (AMCs); and DOXO + AMC.(e) IL-10. Data are averaged (n ≥ 04 ± SEM) of expression. (%). Comparisions against control were performed with one-way analysis of variance (ANOVA) as a Bonferroni post-hoc test, *p <0.05, **p <0.01, ***p <0.001.

From: Borghesi J, Caceres S, Mario LC, Alonso-Diez A, Silveira Rabelo AC, Illera MJ, Silvan G, Miglino MA, Favaron PO, Carreira ACO, Illera JC.
Effects of doxorubicin associated with amniotic membrane stem cells in the treatment of canine inflammatory breast carcinoma (IPC-366) cells.
BMC Vet Res. 2020 Sep 24;16(1):353.
doi: 10.1186/s12917-020-02576-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine Interleukin-10 Antibody, clone CC320 gallery image 3
Published customer image:
Biotinylated Mouse anti Bovine IL-10 antibody, clone CC320 (MCA2111B) used as a detection antibody for the evaluation of IL-10 levels in ovine samples by ELISA in conjunction with Mouse anti Bovine IL-10 antibody, clone CC318 (MCA2110) as a capture reagent.
Image caption:
Time-course of changes in circulating interleukins. Time-course of changes in circulating interleukin IL-10 in the control (open circles, n = 7), LPS (red circles, n = 8) and ET + LPS (blue circles, n = 8) groups. The light shaded area represents the period of low dose LPS infusion, the dark shaded area represents the period of increased infusion. The dashed vertical lines show the timing of LPS/saline bolus administration. Data are hourly means ± SEM. LPS, lipopolysaccharide, ET, Etanercept. a = P <0.05 LPS vs. control, b = P <0.05 ET + LPS vs. control, c = P <0.05 LPS vs. ET + LPS

From: Galinsky R, Dhillon SK, Dean JM, Davidson JO, Lear CA, Wassink G, Nott F, Kelly SB, Fraser M, Yuill C, Bennet L, Gunn AJ.
Tumor necrosis factor inhibition attenuates white matter gliosis after systemic inflammation in preterm fetal sheep.
J Neuroinflammation. 2020 Mar 23;17(1):92.
doi: 10.1186/s12974-020-01769-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine Interleukin-10 Antibody, clone CC320 gallery image 4
Published customer image:
Biotinylated Mouse anti Bovine IL-10 antibody, clone CC320 (MCA2111B) used to assess IL-6 levels in plasma samples by ELISA.
Image caption:
Effect of IA LPS and IV hAECs on systemic cytokine expression. Fetal plasma IL-6 (A) and IL-10 (B) concentrations were not significantly different between groups. Data are expressed as mean ± SEM.

From: Westover A, Melville JM, McDonald C, Lim R, Jenkin G, Wallace EM, Moss TJ.
Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
Front Physiol. 2017 Nov 6;8:871.
doi: 10.3389/fphys.2017.00871.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine Interleukin-10 Antibody, clone CC320 gallery image 5
Published customer image:
Mouse anti Bovine Interleukin-10 antibody, clone CC318 (MCA2110) used as a capture reagent in a sandwich ELISA with biotinylated Mouse anti Bovine IL-10 antibody, clone CC320 (MCA2111B) as a detection reagent for the measurement of IL-10 secretion by bovine PBMC.
Image caption:
Interleukin (IL)-10 secretion by PBMCs unstimulated (UNC) and stimulated with LPS and ConA (SC, 1 and 5 μg/ml, respectively) and with 4 mM of H2O2 for 24 h at 37°C/5% CO2. *P <0.05 were considered significant among feeding strategy.

From: Ciliberti MG, Albenzio M, De Palo P, Santillo A, Caroprese M.
Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
Front Vet Sci. 2020 Feb 20;7:9.
doi: 10.3389/fvets.2020.00009.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Bovine Interleukin-10:Biotin

Product Type
Monoclonal Antibody
Clone
CC320
Isotype
IgG1
Specificity
IL-10

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Mouse anti Bovine Interleukin-10 antibody, clone CC320 recognizes bovine IL-10.

Mouse anti Bovine Interleukin-10 antibody, clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Plasmid cDNA encoding bovine IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2ug/ml 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This biotin conjugate may be used as detection reagent in a sandwich ELISA assay for bovine IL-10 with MCA2110 as capture reagent, see Bannerman, D.D.et al.

References for IL-10 antibody

  1. Hoek, A. et al. (2009) Subpopulations of bovine WC1(+) gammadelta T cells rather than CD4(+)CD25(high) Foxp3(+) T cells act as immune regulatory cells ex vivo.
    Vet Res. 40:6.
  2. Denis, M. et al. (2007) Enhancement of the sensitivity of the whole-blood gamma interferon assay for diagnosis of Mycobacterium bovis infections in cattle.
    Clin Vaccine Immunol. 14: 1483-9.
  3. Flynn, R.J. and Mulcahy, G. (2008) Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages.
    Infect Immun. 76: 678-84.
  4. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
    J Immunol. 174: 6702-15.
  5. Bannerman, D.D. et al. (2004) Characterization of the bovine innate immune response to intramammary infection with Klebsiella pneumoniae.
    J Dairy Sci. 87: 2420-32.
  6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  7. Berger, S.T. and Griffin, F.T. (2006) A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
    Immunol Cell Biol. 84: 349-56.
  8. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
    Vet Res. 41: 14.
    9. View The Latest Product References
  9. Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.
    Biol Reprod. 77: 914-22.
  10. Dean, G.S. et al. (2008) Isoniazid treatment of Mycobacterium bovis in cattle as a model for human tuberculosis.
    Tuberculosis (Edinb). 88: 586-94.
  11. Guan, G. et al. (2010) Course of infection by Babesia sp. BQ1 (Lintan) and B. divergens in sheep depends on the production of IFNgamma and IL10.
    Parasite Immunol. 32: 143-52.
  12. Karcher, E.L. et al. (2008) Osteopontin: a novel cytokine involved in the regulation of Mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle.
    J Dairy Sci. 91: 3079-91.
  13. Lei, L. and Hostetter, J.M. (2007) Limited phenotypic and functional maturation of bovine monocyte-derived dendritic cells following Mycobacterium avium subspecies paratuberculosis infection in vitro.
    Vet Immunol Immunopathol. 120: 177-86.
  14. Parker, D.G. et al. (2009) A steroid-inducible promoter for the cornea.
    Br J Ophthalmol. 93: 1255-9.
  15. Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
    J Immunol. 180: 5963-72.
  16. Souza, C.D. et al. (2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    Microbes Infect. 9: 1545-52.
  17. Stabel, J.R. and Robbe-Austerman, S. (2011) Early immune markers associated with Mycobacterium avium subsp. paratuberculosis infection in a neonatal calf model.
    Clin Vaccine Immunol. 18: 393-405.
  18. Van Rhijn, I. et al. (2007) Massive, sustained gammadelta T cell migration from the bovine skin in vivo.
    J Leukoc Biol. 81: 968-73.
  19. Yamaguchi, T. et al. (2010) Generation of IFN-gamma-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines.
    Vet Parasitol. 171: 207-15.
  20. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
    Vet Immunol Immunopathol. 85 (3-4): 213-23.
  21. Buza JJ et al. (2004) Neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with Johnin purified protein derivative and by infection with Mycobacterium avium subsp. paratuberculosis in experimentally infected cattle with paratuberculosis.
    Infect Immun. 72 (4): 2425-8.
  22. Scandurra, G.M. et al. (2010) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
    Infect Immun. 78 (3): 1383-9.
  23. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83 (1): 48-55.
  24. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
    Int Arch Allergy Immunol. 144: 325-37.
  25. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  26. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  27. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
    Clin Experiment Ophthalmol. 38: 405-13.
  28. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  29. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
    Clin Vaccine Immunol. 16 (7): 1003-11.
  30. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  31. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
    J Virol. 82: 1777-86.
  32. Ma&347;lanka T et al. (2012) The presence of CD25 on bovine WC1+ gammadelta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma.
    Pol J Vet Sci. 15 (1): 11-20.
  33. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
    J Comp Pathol. 148: 157-72.
  34. Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
    PLoS One. 10 (5): e0124144.
  35. Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.
    PLoS One. 11 (4): e0154172.
  36. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
    Infect Immun. 85 (2)Jan 26 [Epub ahead of print].
  37. Pomeroy, B. et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.
    Vet Immunol Immunopathol. 182: 37-42.
  38. Pomeroy, B. et al. (2015) Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion.
    Vet Immunol Immunopathol. 167 (1-2): 22-9.
  39. Davidson, J.O. et al. (2021) Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep.
    Stem Cells Transl Med. 10 (3): 427-40.
  40. Borghesi, J. et al. (2020) Effects of doxorubicin associated with amniotic membrane stem cells in the treatment of canine inflammatory breast carcinoma (IPC-366) cells.
    BMC Vet Res. 16 (1): 353.
  41. Galinsky, R. et al. (2020) Tumor necrosis factor inhibition attenuates white matter gliosis after systemic inflammation in preterm fetal sheep.
    J Neuroinflammation. 17 (1): 92.
  42. Westover, A. et al. (2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
    Front Physiol. 8: 871.
  43. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-7.
  44. Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
    Vet Immunol Immunopathol. 211: 44-8.
  45. Stabel, J.R. & Bannantine, J.P. (2019) Divergent Antigen-Specific Cellular Immune Responses during Asymptomatic Subclinical and Clinical States of Disease in Cows Naturally Infected with Mycobacterium avium. subsp. paratuberculosis.
    Infect Immun. 88(1):e00650-19.
  46. Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii.i and M. bovis..
    Vet Immunol Immunopathol. 237: 110268.
  47. Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
    Front Vet Sci. 7: 9.
  48. Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model.
    J Dairy Sci. Mar 17 [Epub ahead of print].

ELISA

ELISpot

Flow Cytometry

Functional Assays

RRID
AB_2125237
UniProt
P43480
Entrez Gene
IL10
GO Terms
GO:0002237 response to molecule of bacterial origin
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042095 interferon-gamma biosynthetic process
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
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