Defensin Beta 2 antibody
Goat anti Human Defensin beta 2 polyclonal antibody recognizes human Defensin beta-2 (BD-2), a ~4.3kDa cationic antimicrobial peptide, also known as Beta-defensin 4A or Skin-antimicrobial peptide 1 (SAP-1) expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections.
Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-Terminal active portion of the protein which is secreted by the neutrophil and binds to bacterial membranes causing their disruption.
Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-Terminal active portion of the protein which is secreted by the neutrophil and binds to bacterial membranes causing their disruption.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Preparation
- Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Preparation
- Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human Defensin beta-2 (PHP161).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- O15263
- Entrez Gene
- DEFB4A
- GO Terms
- GO:0042742 defense response to bacterium
- GO:0005576 extracellular region
- GO:0006935 chemotaxis
- GO:0006955 immune response
- GO:0007186 G-protein coupled receptor protein signaling pathway
- Regulatory
- For research purposes only
Applications of Defensin Beta 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0 ug/ml | |
| Western Blotting | 0.1 | 0.2 ug/ml | |
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 5.0 | 8.0ug/ml | |
| Immunohistology - Paraffin | |||
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This biotinylated human DB-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human BD-2 antibody (AHP849) as the capture reagent and recombinant human BD-2 (PHP161) as the standard.
- ELISA
- This purified human BD-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human BD-2 antibody (AHP849B) as the detection reagent and recombinant human BD-2 (PHP161) as the standard
- Western Blotting
- This biotinylated human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.
- Western Blotting
- This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Defensin Beta 2 antibody
-
Crack, L.R. et al. (2012) Human antimicrobial peptides LL-37 and human β-defensin-2 reduce viral replication in keratinocytes infected with varicella zoster virus.
Clin Exp Dermatol. 37 (5): 534-43.
Further Reading
-
Bals, R. et al. (1998) Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung.
J Clin Invest. 102 (5): 874-80. -
Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia.
Am J Respir Cell Mol Biol. 22 (6): 714-21.
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