Defensin Beta 2 antibody

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Goat anti Human Defensin Beta 2:Biotin

Goat anti Human Defensin Beta 2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP849B E WB 50 µg
AHP849 E FN P WB 0.1 mg
Goat anti Human Defensin beta 2 polyclonal antibody recognizes human Defensin beta-2 (BD-2), a ~4.3kDa cationic antimicrobial peptide, also known as Beta-defensin 4A or Skin-antimicrobial peptide 1 (SAP-1) expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections.

Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-Terminal active portion of the protein which is secreted by the neutrophil and binds to bacterial membranes causing their disruption.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preparation
Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Preparation
Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human Defensin beta-2 (PHP161).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
O15263 Related reagents
Entrez Gene
DEFB4A Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0007186 G-protein coupled receptor protein signaling pathway
Regulatory
For research purposes only

Applications of Defensin Beta 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0 ug/ml
Western Blotting 0.1 0.2 ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 5.0 8.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human DB-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human BD-2 antibody (AHP849) as the capture reagent and recombinant human BD-2 (PHP161) as the standard.
ELISA
This purified human BD-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human BD-2 antibody (AHP849B) as the detection reagent and recombinant human BD-2 (PHP161) as the standard
Western Blotting
This biotinylated human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.
Western Blotting
This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Defensin Beta 2 antibody

  1. Crack, L.R. et al. (2012) Human antimicrobial peptides LL-37 and human β-defensin-2 reduce viral replication in keratinocytes infected with varicella zoster virus.
    Clin Exp Dermatol. 37 (5): 534-43.

Further Reading

  1. Bals, R. et al. (1998) Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung.
    J Clin Invest. 102 (5): 874-80.
  2. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia.
    Am J Respir Cell Mol Biol. 22 (6): 714-21.