Defensin Beta 2 antibody

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Anti Human Defensin Beta 2 Antibody thumbnail image 2

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Anti Human Defensin Beta 2 Antibody gallery image 1 — Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-2 (**AHP849**)

Anti Human Defensin Beta 2 Antibody gallery image 2 — Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-2 (**AHP849**)

Anti Human Defensin Beta 2 Antibody gallery image 3 — Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-2 (**AHP849**)

Anti Human Defensin Beta 2 Antibody gallery image 4 — Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-2 (**AHP849**)

Goat anti Human Defensin Beta 2:Biotin

Goat anti Human Defensin Beta 2

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP849B	E WB			50 µg
AHP849	E FN P WB			0.1 mg

Goat anti Human Defensin beta 2 polyclonal antibody recognizes human Defensin beta-2 (BD-2), a ~4.3kDa cationic antimicrobial peptide, also known as Beta-defensin 4A or Skin-antimicrobial peptide 1 (SAP-1) expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections.

Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-Terminal active portion of the protein which is secreted by the neutrophil and binds to bacterial membranes causing their disruption.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Biotin - lyophilised
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution: Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preparation: Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Preparation: Antisera to human Defensin beta 2 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Preservative Stabilisers: None present
Immunogen: Recombinant human Defensin beta-2 (PHP161).
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml after reconstitution

Storage Information

Storage: Prior to reconstitution store at +4^oC.
Following reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
Following reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: O15263 Related reagents
Entrez Gene: DEFB4A Related reagents
GO Terms: GO:0042742 defense response to bacterium; GO:0005576 extracellular region; GO:0006935 chemotaxis; GO:0006955 immune response; GO:0007186 G-protein coupled receptor protein signaling pathway
Regulatory: For research purposes only

Applications of Defensin Beta 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.25	1.0 ug/ml
Western Blotting	0.1	0.2 ug/ml
ELISA	0.5	2.0ug/ml
Functional Assays	5.0	8.0ug/ml
Immunohistology - Paraffin
Western Blotting	0.1	0.2ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: This biotinylated human DB-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human BD-2 antibody (AHP849) as the capture reagent and recombinant human BD-2 (PHP161) as the standard.
ELISA: This purified human BD-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human BD-2 antibody (AHP849B) as the detection reagent and recombinant human BD-2 (PHP161) as the standard
Western Blotting: This biotinylated human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.
Western Blotting: This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 (PHP161) as the positive control.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

Product Specific References

References for Defensin Beta 2 antibody

Crack, L.R. et al. (2012) Human antimicrobial peptides LL-37 and human β-defensin-2 reduce viral replication in keratinocytes infected with varicella zoster virus.
Clin Exp Dermatol. 37 (5): 534-43.