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Anti-MMAE Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Antibodies for the Detection of Monomethyl Auristatin E (MMAE)
Sensitive assays for detection of monomethyl auristatin E (MMAE) and for antibody drug conjugates (ADC) incorporating this toxin. Antibodies also bind to the related toxins MMAD and MMAF.
- Recombinant antibodies specific to MMAE. Antibodies also bind with similar sensitivity to MMAD. MMAF is also recognized, but with lower sensitivity
- Sequence-defined, well characterized reagents with secure supply
- Stringent quality control for batch-to-batch consistency
- Nonanimal-derived antibodies, reducing the use of animals in science
Table 1. Antibodies Specific for MMAE
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
|---|---|---|---|---|---|---|
|
MMAE (free and conjugated) |
TZA056 |
AbD50347ad |
Fab-F-Spy2-H1 |
3 |
PK bridging ELISA (capture) |
|
|
TZA057 |
AbD50348ad |
Fab-F-Spy2-H1 |
5 |
PK bridging ELISA (detection with free choice of SpyCatcher label) |
||
|
TZA57P |
AbD50348pap |
Fab2-FH-X22-HRP2 |
5 |
PK bridging ELISA (detection with HRP) |
* Affinity measured in the monovalent Fab format
1 Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags
2 Fab2-FH-X22-HRP= Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags and Fab-SpyTag coupled to HRP conjugated BiCatcher
Human Anti-MMAE Antibody (TZA056) inhibition ELISA against MMAE, MMAF, MMAD (Data for each cytotoxic payload were obtained from separate experiments and combined in one graph).
Anti-MMAE Antibodies
The monomethyl auristatin E (MMAE) toxin is a very potent antimitotic agent used as a payload for developing antibody-drug conjugates (ADC) blocking cell division.
The anti-MMAE antibodies bind MMAE in both its free and conjugated form. They are also reactive to the closely related toxins MMAD and MMAF.
They are ideal for the development of pharmacokinetic (PK) bridging ELISAs to measure antibody drugs conjugated to those toxins.
ELISA Protocols to Get You Started
Fig. 1. MMAE conjugated antibody Brentuximab vedotin PK bridging ELISA using antibodies TZA056 and TZA057P.