SDF-1 Alpha antibody

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Rabbit anti Human SDF-1 Alpha:Biotin

Rabbit anti Human SDF-1 Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP794B E WB 50 µg
AHP794 E FN P* WB 0.1 mg
Rabbit anti Human SDF-1 alpha antibody recognizes human SDF-1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform.

SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human SDF-1α were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human SDF-1α
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P48061 Related reagents
Entrez Gene
CXCL12 Related reagents
GO Terms
GO:0009615 response to virus
GO:0007155 cell adhesion
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006874 cellular calcium ion homeostasis
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008009 chemokine activity
GO:0008015 blood circulation
GO:0008064 regulation of actin polymerization or depolymerization
GO:0008083 growth factor activity
GO:0090026 positive regulation of monocyte chemotaxis
Regulatory
For research purposes only

Applications of SDF-1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 2.0 4.0ug/ml
Immunohistology - Paraffin 1 0.5ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human SDF-1α antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human SDF-1α antibody (AHP794) as the capture reagent and recombinant human SDF-1α (PHP122) as the standard.
ELISA
This purified human SDF-1α antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human SDF-1α antibody (AHP794B) as the detection reagent and recombinant human SDF-1α (PHP122) as the standard
Histology Positive Control Tissue
Human colorectal adenocarcinoma
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1α (PHP122) as the positive control.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1α (PHP122) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Human SDF-1 Alpha AHP794 0.1 mg E FN P* WB
Rabbit anti Human SDF-1 Alpha:Biotin AHP794B 50 µg E WB
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Positive Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human SDF-1 Alpha PHP122 10 µg E FN WB
Recombinant Human SDF-1 Alpha PHP122 10 µg E FN WB

Product Specific References

References for SDF-1 Alpha antibody

  1. Wuchter, P. et al. (2016) Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells.
    Cell Tissue Res. 364 (3): 573-84.
  2. Song, J. et al. (2015) Focal MMP-2 and MMP-9 activity at the blood-brain barrier promotes chemokine-induced leukocyte migration.
    Cell Rep. 10 (7): 1040-54.

Further Reading

  1. Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV infection.
    Semin Immunol. 10 (3): 179-85.
  2. Stumm, R. & Höllt, V. (2007) CXC chemokine receptor 4 regulates neuronal migration and axonal pathfinding in the developing nervous system: implications for neuronal regeneration in the adult brain.
    J Mol Endocrinol. 38 (3): 377-82.
  3. Kucia, M. et al. (2005) Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis.
    Stem Cells. 23 (7): 879-94.