TNF Alpha antibody | CC327

Mouse anti Bovine TNF Alpha

Product Type
Monoclonal Antibody
Clone
CC327
Isotype
IgG2b
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA2334
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Bovine TNF alpha antibody, clone CC327 recognizes bovine TNF alpha, a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.

The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Fallow deer
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant bovine TNF alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
This reagent may be used as a capture antibody in a sandwich ELISA for bovine TNF alpha in combination with MCA2335B as detection reagent. PBP005 may be used as a standard.

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References for TNF Alpha antibody

  1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.
    Vet Immunol Immunopathol. 95 (1-2): 21-31.
  2. Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.
    Infect Immun. 71 (11): 6420-5.
  3. Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaβ activation of Theileria parva-transformed B cells.
    Cell Microbiol. 5 (10): 709-16.
  4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumournecrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.
    Vet Immunol Immunopathol. 135: 320-4.
  5. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  6. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  7. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
    Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
  8. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  9. View The Latest Product References
  10. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
    PLoS One. 6: e29194.
  11. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
    Vet Immunol Immunopathol. 149: 66-75.
  12. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  13. Luisa, C. et al. (2016) Evaluation of serum markers of blood redox homeostasis and inflammation in PCB naturally contaminated heifers undergoing decontamination
    Science of The Total Environment. 542: 653-64.
  14. Maggioli, M.F. et al. (2016) Increased TNF-α/IFN-γ/IL-2 and Decreased TNF-α/IFN-γ Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination.
    Front Immunol. 7: 421.
  15. Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
    Biol Reprod. 95 (2): 39.
  16. Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.
    Vet Immunol Immunopathol. 162 (3-4): 192-7.
  17. Jolly A et al. (2016) Evidence of a pro-apoptotic effect of specific antibodies in a bovine macrophage model of infection with Mycobacterium avium subsp. paratuberculosis.
    Vet Immunol Immunopathol. 169: 47-53.
  18. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-7.
  19. Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
    Front Vet Sci. 8: 698800.

ELISA

RRID
AB_2256048
UniProt
Q06599
Entrez Gene
TNF
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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