TNF Alpha antibody | CC327
Phycoerythrin conjugated Mouse anti Bovine TNF alpha antibody, clone CC327 (MCA2334) used for the evaluation of TNF-α expression by bovine PBMC following M. bovis infection.
Image caption:
Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN-γ, IL-2 and TNF-α determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN-γ, IL-2 or TNF-α following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN-γ+ and IFN-γ− CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle.
From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine TNF alpha antibody, clone CC327 (MCA2334) used as a capture reagent for the evaluation of TNF production using a sandwich ELISA with biotinylated clone CC328 (MCA2335B) as a detection reagent.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-⃝-) and S. simulans (-&9689;-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.
From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine TNF alpha antibody, clone CC327 (MCA2334) used as a capture reagent for the evaluation of TNF production using a sandwich ELISA with biotinylated clone CC328 (MCA2335B) as a detection reagent.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-□-) and spontaneously eliminated (-■-) S. epidermidis and S. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.
From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine TNF alpha antibody, clone CC327 (MCA2334) used to evaluate TNF-α expression in bovine granulomatous lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunolabelling for TNF-α+ (ABC IHC stain, DAB brown chromogen). (A) Comparison of TNF-α+ distribution in different granuloma stages of BCG vaccinated (Vac) and non-vaccinated (Con) calves. (B) Stage I granuloma from non-vaccinated calf with few number of TNF-α stained macrophages (arrows), (C) Stage I from BCG vaccinated calf with more positively stained macrophages (arrows). (D) Stage IV granuloma from non-vaccinated calf with moderate number of positive cells (arrows). (E) Stage IV granuloma from BCG vaccinated calf with abundance of positively stained cells (arrows). Bar equals 100μm in (B,C), and 50μm in (D,E). n, necrotic core.
From: Sirak A, Tulu B, Bayissa B, Gumi B, Berg S, Salguero FJ, Ameni G; ETHICOBOTS Consortium.
Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 2021 Sep 16;8:698800.
doi: 10.3389/fvets.2021.698800.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F E ResetMouse anti Bovine TNF Alpha
- Product Type
- Monoclonal Antibody
- Clone
- CC327
- Isotype
- IgG2b
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Bovine TNF alpha antibody, clone CC327 recognizes bovine TNF alpha, a 17.5 kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Fallow deer - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine TNF alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry 1 | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for TNF Alpha antibody
-
Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.
Vet Immunol Immunopathol. 95 (1-2): 21-31. -
Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.
Infect Immun. 71 (11): 6420-5. -
Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaβ activation of Theileria parva-transformed B cells.
Cell Microbiol. 5 (10): 709-16. -
Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumournecrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.
Vet Immunol Immunopathol. 135: 320-4. -
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. -
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. -
Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24. -
Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 42: 49.
View The Latest Product References
-
Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194. -
García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
Vet Immunol Immunopathol. 149: 66-75. -
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48. -
Luisa, C. et al. (2016) Evaluation of serum markers of blood redox homeostasis and inflammation in PCB naturally contaminated heifers undergoing decontamination
Science of The Total Environment. 542: 653-64. -
Maggioli, M.F. et al. (2016) Increased TNF-α/IFN-γ/IL-2 and Decreased TNF-α/IFN-γ Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination.
Front Immunol. 7: 421. -
Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.
Biol Reprod. 95 (2): 39. -
Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.
Vet Immunol Immunopathol. 162 (3-4): 192-7. -
Jolly A et al. (2016) Evidence of a pro-apoptotic effect of specific antibodies in a bovine macrophage model of infection with Mycobacterium avium subsp. paratuberculosis.
Vet Immunol Immunopathol. 169: 47-53. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7. -
Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 8: 698800.
- RRID
- AB_2256048
- UniProt
- Q06599
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
MCA2334
0514
147543
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