Knockdown (siRNA) Validated Antibodies

PrecisionAb AntibodiesHighly Validated Antibodies For Results You Can Trust

In accordance with recommendations by the International Working Group for Antibody Validation (Uhlen et al. 2016), we use various methods to validate PrecisionAb Antibodies. Our methods include Knockdown (siRNA) antibody validation.

siRNA Validation

These include genetic strategies where we utilize small interfering RNA (siRNA) to knock down gene expression (Figure 1).

The downregulation of the target protein confirms specificity.

Fig. 1. Western blot analysis of PFKP knockout HeLa (PFKP KO) and wild type HeLa (WT) whole cell lysates.

Fig. 1. Small interfering RNA (siRNA) used to knock down gene expression. A, western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against H-Ras (1 and 2) or a scrambled siRNA (-) probed with Mouse Anti H-Ras Antibody (MCA2884) and hFAB Rhodamine Anti-Tubulin Primary Antibody (12004166). B, normalized signal intensity of H-Ras to the tubulin signal.

Knockdown (KD) Validated PrecisionAb Antibodies

Antibodies that have been knockdown (siRNA) validated are marked with a KD validation flag and a PrecisionAb enhanced validation logo on product webpages.

Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.

Our KD Validated Antibody Range (PrecisionAbs)

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code Validation Types

    Choosing Validated Antibodies for Trustworthy DataChoosing Validated Antibodies for Trustworthy Data

    Learn about the validation methods implemented by Bio-Rad, including CRISPR-Cas9 gene editing, siRNA, and immunoprecipitation followed by mass spectrometry, ensuring the specificity and reliability of our antibodies in your application.

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    Reference

    • Uhlen M et al. (2016). A proposal for validation of antibodies. Nature Methods 13, 823-827.

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