In accordance with recommendations by the International Working Group for Antibody Validation (Uhlen et al. 2016), we use various methods to validate PrecisionAb Antibodies. Our methods include Knockdown (siRNA) antibody validation.
These include genetic strategies where we utilize small interfering RNA (siRNA) to knock down gene expression (Figure 1).
The downregulation of the target protein confirms specificity.
Fig. 1. Small interfering RNA (siRNA) used to knock down gene expression. A, western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against H-Ras (1 and 2) or a scrambled siRNA (-) probed with Mouse Anti H-Ras Antibody (MCA2884) and hFAB Rhodamine Anti-Tubulin Primary Antibody (12004166). B, normalized signal intensity of H-Ras to the tubulin signal.
Antibodies that have been knockdown (siRNA) validated are marked with a KD validation flag and a PrecisionAb enhanced validation logo on product webpages.
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|Description||Specificity||Target||Format||Host||Isotype||Clone||Applications||Citations||Product Type||Code||Validation Types|
Learn about the validation methods implemented by Bio-Rad, including CRISPR-Cas9 gene editing, siRNA, and immunoprecipitation followed by mass spectrometry, ensuring the specificity and reliability of our antibodies in your application.
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