IgG2a antibody | LO-MG2a-9

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Rat anti Mouse IgG2a Heavy Chain:FITC

Rat anti Mouse IgG2a Heavy Chain:HRP

Rat anti Mouse IgG2a Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MG2a-9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1588F F 0.5 mg
MCA1588P E 0.5 mg
MCA1588 E 1 mg
Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 recognizes the mouse gamma 2a heavy chain, binding to all allotypes and does not cross-react with other murine immunoglobulin classes or subclasses.

The avidity of Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 for murine IgG2a = 2.5 x 109M-1.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified IgG2a from Balb/c mice. PTR
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01865 Related reagents
P01864 Related reagents
P01863 Related reagents
Entrez Gene
Igh-1a Related reagents
Igh-1a Related reagents
Igh-1a Related reagents
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of IgG2a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 5ug/ml
ELISA 250ng/ml
ELISA 5ug/ml as coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MG2a-7 (MCA421P) as the detection reagent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:FITC MCA1211F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgG2a antibody

  1. Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies.
    PLoS One. 5(4):e10105.
  2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  3. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
    Vaccine. 24: 4102-10.
  4. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels.
    J Allergy Clin Immunol. 122: 633-9.e3.
  5. Carew, J.S. et al. (2015) Targeting Survivin Inhibits Renal Cell Carcinoma Progression and Enhances the Activity of Temsirolimus.
    Mol Cancer Ther. 14 (6): 1404-13.
  6. Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
    PLoS One. 9 (6): e100392.