IgG2a antibody | LO-MG2a-9
Rat anti Mouse IgG2a Heavy Chain:FITC
Rat anti Mouse IgG2a Heavy Chain:HRP
Rat anti Mouse IgG2a Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MG2a-9
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1588F | F | 0.5 mg |
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MCA1588P | E | 0.5 mg |
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MCA1588 | E | 1 mg |
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The avidity of Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 for murine IgG2a = 2.5 x 109M-1.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
0.01% Thiomersal 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified IgG2a from Balb/c mice. PTR
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01865
- P01864
- P01863
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of IgG2a antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 5ug/ml | ||
ELISA | 250ng/ml | ||
ELISA | 5ug/ml as coating antibody |
- ELISA
- This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MG2a-7 (MCA421P) as the detection reagent.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG2a antibody
-
Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies.
PLoS One. 5(4):e10105. -
Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36. -
Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
Vaccine. 24: 4102-10. -
Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels.
J Allergy Clin Immunol. 122: 633-9.e3. -
Carew, J.S. et al. (2015) Targeting Survivin Inhibits Renal Cell Carcinoma Progression and Enhances the Activity of Temsirolimus.
Mol Cancer Ther. 14 (6): 1404-13. -
Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
PLoS One. 9 (6): e100392. -
Islam, S. et al. (2020) Resistance to histone deacetylase inhibitors confers hypersensitivity to oncolytic reovirus therapy.
Blood Adv. 4 (20): 5297-310.
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