IgG2a antibody | LO-MG2a-9
Horseradish peroxidase conjugated Rat anti Mouse IgG2a antibody, clone LO-MGSa-9 antibody (MCA1588P) used as a tertiary detection antibody for the detection of bound mouse IgG2a antibody in an antibody response ELISA following BSRV challenge.
Image caption:
Mucosal IgA antibodies in the upper and lower airways, before and after BRSV challenge.
Four groups of 5 calves were vaccinated as described in Fig. 1 and challenged with BRSV, 5 weeks after vaccination. Two weeks before challenge, one calf (c5) was euthanized due to traumatic injury. BRSV-specific IgA antibodies were analyzed by ELISA. (A) shows group mean levels of BRSV-specific IgA in nasal secretions on post-infection day (PID) 0 and 7, whereas (B) and (C) show group mean titers of total BRSV- and HRSV-N-specific IgA in bronchoalveolar lavage (BAL) on PID 7, respectively. BAL samples were titrated, whereas antibody levels in nasal secretions were semi-quantitatively determined and expressed as a percentage of a positive control sample, due to lack of sample material. Standard deviations are indicated by upward deflecting lines. Statistically significant differences between PID 0 and PID 7 in panel A are indicated by a horizontal line, whereas in all panels significant differences between groups for the same time-point are indicated by asterisks and the corresponding group letter; p≤0.05 (*); p≤0.01 (**); p≤0.005 (***); p≤0.001 (****).
From: Blodörn K, Hägglund S, Fix J, Dubuquoy C, Makabi-Panzu B, Thom M, et al. (2014)
Vaccine Safety and Efficacy Evaluation of a Recombinant Bovine Respiratory Syncytial Virus (BRSV) with Deletion of the SH Gene and Subunit Vaccines Based On Recombinant Human RSV Proteins: N-nanorings, P and M2-1, in Calves with Maternal Antibodies.
PLoS ONE 9(6): e100392.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
E ResetRat anti Mouse IgG2a Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MG2a-9
- Isotype
- IgG1
- Specificity
- IgG2a
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 recognizes the mouse gamma 2a heavy chain, binding to all allotypes and does not cross-react with other murine immunoglobulin classes or subclasses. The avidity of Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-9 for murine IgG2a = 2.5 x 109M-1. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% sodium azide (NaN3)
- Immunogen
- Purified IgG2a from Balb/c mice. PTR
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml as coating antibody |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MG2a-7 (MCA421P) as the detection reagent.
References for IgG2a antibody
-
Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies.
PLoS One. 5(4):e10105. -
Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36. -
Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
Vaccine. 24: 4102-10. -
Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels.
J Allergy Clin Immunol. 122: 633-9.e3. -
Carew, J.S. et al. (2015) Targeting Survivin Inhibits Renal Cell Carcinoma Progression and Enhances the Activity of Temsirolimus.
Mol Cancer Ther. 14 (6): 1404-13. -
Blodörn, K. et al. (2014) Vaccine safety and efficacy evaluation of a recombinant bovine respiratory syncytial virus (BRSV) with deletion of the SH gene and subunit vaccines based on recombinant human RSV proteins: N-nanorings, P and M2-1, in calves with maternal antibodies.
PLoS One. 9 (6): e100392. -
Islam, S. et al. (2020) Resistance to histone deacetylase inhibitors confers hypersensitivity to oncolytic reovirus therapy.
Blood Adv. 4 (20): 5297-310.
- RRID
- AB_321821
- UniProt
- P01865
- P01864
- P01863
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
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