Our comprehensive library of videos is designed to inform you of best practices when using antibodies in the following areas and applications:


Custom Services

Custom Services

Designed Just for You - Discover the Technology behind Bio-Rad's Custom Recombinant Antibodies

See inside Bio-Rad’s custom antibody facility and learn from antibody experts how your requirements for specialized custom antibodies can become a reality through the use of HuCAL® technology.

Duration: 5 min
 

TrailBlazer Antibodies Enable Site-Specific Conjugation and Fast Format Conversion

Discover how SpyTag-SpyCatcher technology has been combined with antibody phage display to make TrailBlazer Antibodies. See how this innovation enables site-directed conjugation with controlled degree of labeling and lot-to-lot consistency, plus fast antibody assembly into multiple stable formats. Improve assay design, performance, and reproducibility.

Duration: 2 min
 

Anti-Idiotypic Antibodies for Development of CAR T Cell Therapies and Monoclonal Antibody Drugs

This video explains the challenges associated with the development of CAR T cell therapies that can be addressed using anti-idiotypic antibodies.

Speaker:  Michael Schwenkert, Technical Sales Manager, Bio-Rad
Duration: 3 min
 

Drug-Target Complex Specific Antibodies for Biotherapeutic Development

Michael Schwenkert describes how high sensitivity PK assays can be developed using specialized drug-target complex specific antibodies, avoiding the ELISA bridging format; recorded at the EBF 10th Open Meeting (Barcelona, November 2017).

Speaker:  Michael Schwenkert, Technical Sales Manager, Bio-Rad
Duration: 6 min
 

Best Practices for Characterization and QC of Anti-Idiotypic Antibodies for Bioanalysis

Hear how Bio-Rad’s recombinant anti-idiotypic antibodies, made using HuCAL technology, fulfill the high quality standards demanded for use in assays to support the clinical evaluation of monoclonal antibody drugs; recorded at the EBF 9th Open Meeting.

Speaker:  Michael Schwenkert, Technical Sales Manager, Bio-Rad
Duration: 8 min
 

Generating Anti-Idiotypic Antibodies for Bioanalytical Assays

Dr Christian Frisch explains how we generate anti-idiotypic antibodies for bioanalytical assay development using the HuCAL recombinant antibody technology; recorded at the EBF 8th Open Meeting.

Speaker:  Dr Christian Frisch, R&D Manager, Bio-Rad
Duration: 8 min
 

Flow Cytometry

Flow Cytometry

StarBright Dyes for Flow Cytometry

StarBright Dyes are new, proprietary fluorescent nanoparticles conjugated to Bio-Rad's highly validated antibodies. This video shows how they can be used in an immunophenotyping panel to obtain improved results compared to conventional dyes.

Duration: 2 min
 

General applications

General Applications

No Experiment Is Complete without All the Pieces

Overview of our antibody range and new resources for key applications including flow cytometry, western blotting, IF/ICC, and IHC to help you improve and optimize your experiments.

Duration: 1 min
 

Smarter Antibody Search with A.I.den from Bio-Rad

Finding the tools you need for your experiments shouldn’t be a hassle. Our smart search engine, A.I.den, is powered by cutting edge A.I. and machine learning technology. Meet A.I.den and learn about the benefits of our antibody search engine in our new video.

Duration: 1 min
 

Secondary Antibodies

Secondary Antibodies

Bio-Rad's Isotype Specific Secondary Antibodies

Immunohistofluorescence multiplexing is challenging due to cross-reactivity. Attempts to reduce it, like blocking, are usually unsuccessful and lead to false results. We will demonstrate how, by using Bio-Rad's Isotype Specific Secondary Antibodies, you can achieve accurate results you can trust in only two steps.

Duration: 6 min
 

Western Blotting

Western Blotting

Learn How to Perform a Western Blot

This video demonstrates SDS-PAGE separation of proteins using the Bio-Rad Comparative Proteomics Kit II: Western Blot Module. Assembly of the blotting sandwich and electroblotting are shown along with the steps for protein detection using a colorimetric assay.

Duration: 10 min
 

Choosing Validated Antibodies for Trustworthy Data

Discover the comprehensive validation methods implemented by Bio-Rad, including CRISPR-Cas9 gene editing, siRNA, and immunoprecipitation followed by mass spectrometry, ensuring the specificity and reliability of our antibodies in your application.

Duration: 3.51 min
 

Immunoprecipitation Procedure Using SureBeads Magnetic Beads and TidyBlot Reagent Product Range

This video highlights the different steps in an IP procedure, using SureBeads Magnetic Beads, and how you can use TidyBlot Western Blot Detection Reagent to mitigate obstruction from IgG heavy and light chains and generate clean blots.

Duration: 3 min
 

Selecting a Primary Antibody to Get the Best Results in Western Blotting

Knowing the type of validation data and requirements for a high-quality primary antibody is critical to success in western blotting. This video discusses what users should expect, and also what to look out for when selecting their primary antibodies.

Duration: 2 min
 

How to Choose a Primary Antibody for Multiplex Western Blotting

In this video, we discuss the selection of primary antibodies:

  • Choosing primary antibodies from different host species is often the easiest way to multiplex
  • When choosing antibodies from different host species, distantly related species are less likely to have issues with species cross-reactivities. Avoid choosing antibodies from closely related species like mouse and rat
  • Using primary antibodies from the species but different antibody classes like IgG and IgM in combination with appropriate secondary antibodies is one way to overcome species issues
  • Primary antibodies from the same species but of different isotypes such as IgG-1 and IgG-2 provide another possible solution

Duration: 1 min
 

How to Choose Secondary Antibodies for Multiplex Western Blotting

In this video, we discuss the best practices for selecting secondary antibodies:

  • Use the correct secondary antibodies to detect your primary antibodies. This will be a species-specific, class-specific, or isotype-specific approach depending on your multiplexing approach
  • Do not use secondary antibodies raised in the same species as any of your primary antibodies when using a species-specific approach
  • Ensure specificity of your staining by using cross-adsorbed secondary antibodies to prevent cross-reaction with primary antibodies. The cross-adsorbed antibody will correctly bind the target primary antibody but will not cross-react to the primary antibody from the related species
  • Ensure that the different detection methods can be detected by your imager

Duration: 2 min
 

Optimizing the Blocking Step in Western Blotting

Membrane blocking is a crucial step in western blotting because it minimizes nonspecific binding of antibodies to the blotting membrane. Not all blocking agents are equal and optimization is commonly required to get the least background with the best target protein signal.

Duration: 1 min