IFN Gamma antibody | CC330
IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer’s Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.
From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E.
Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 2014 Dec 16;10(1):293.
Image caption:
γδ T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5×104 CFUs of B. abortus 2308, and two weeks later γδ T cells (>95% purity) and an enriched TCRαβ (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean ± SD is shown; * P<0.05 versus the enriched TCRαβ cells. Results are representative of two independent experiments.
From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011)
Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections.
PLoS ONE 6(7): e21978.
Image caption:
γδ T cells require TNF-α to protect against B. abortus infection. C57BL/6 mice treated with anti-TCRγδ mAb or hamster IgG on day -1 and day 3 post-infection with 5×104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-α on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean ± SEM of 10 mice/group is depicted; *P<0.05 versus hamster IgG-treated C57BL/6 mice. Results are from two independent experiments. C. and D. Splenocytes (5×106/ml) from 4 or 7 day B. abortus-infected mice depleted or not of γδ T cells and/or TNF-α or splenocytes from naive C57BL/6 mice were left unstimulated and cultured for 3 days at 37°C/5%CO2; supernatants were harvested for C. TNF-α- or D. IFN-γ- specific ELISA. The mean ± SD of triplicate wells is shown. The results from 7 dpi are representative of two independent experiments. NS = not significant. ND = cytokine production from uninfected mice neutralized of γδ T cells was not determined. * P<0.05 as compared to mice not depleted of γδ T cells within the same TNF-α treatment group at the same time point. † P<0.05 as compared to C57BL/6 mice treated with IgG only at the same time point.
From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.
Image caption:
Bovine γδ T cells impair B. abortus replication in autologous macrophages via IFN-γ. A.–F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous γδ T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P<0.05 versus colonization by cultured macrophages only from the same animal. B.,D.,F. IFN-γ levels were determined in cell culture supernatants. At 72 and 120 h post-infection, macrophage plus γδ T cell co-cultures from each calf contained more IFN-γ than from macrophages cultured alone. G. NK1.1+ cell-depleted Rag-1−/− mice (4–5 per group) received PBS, bovine macrophages (5×105/mouse), bovine macrophages (5×105/mouse) plus autologous γδ T cells (1×107/mouse), or bovine macrophages plus autologous CD4+ T cells (1×107/mouse) i.p. one day prior to infection with 1×104 CFUs of B. abortus. All cells were derived from Calf #1. On day 7, splenic colonization was determined. Depicted is the mean ± SD; *P<0.05 versus mice given PBS or macrophages only.
From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.
Mouse anti bovine interferon gamma antibody, clone CC330 used for the measurement of the gamma interferon response to M. avium infection in a caprine model by ELISA in conjunction with biotinylated Mouse anti Bovine interferon γ antibody, clone CC302 as a detection reagent.
Image caption:
Time course and intensity of IFN-γ response (B) of inoculated and control goats. Box and Whisker Plot represents median value, 25% and 75% percentiles (box), range, outlier values (◯), and extreme values (*). Different letters indicate significant differences between groups (Mann–Whitney-U test, P ≤ 0.05): a – V1 vs. V2, b – V1 vs. V3, c – V1 vs. V4, d – V2 vs. V3, e – V2 vs. V4, f – V3 vs. V4.
From: Köhler H, Soschinka A, Meyer M, Kather A, Reinhold P, Liebler-Tenorio E.
Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.
BMC Vet Res. 2015 Mar 24;11(1):74.
Mouse anti Bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, a 143 amino acid ~20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading microorganisms. Interferon γ enhances cytotoxic activities of many cell types including macrophages, NK cells and T lymphocytes (Weiss et al. 2002).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine IFN-gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P07353 Related reagents
- Entrez Gene
- IFNG Related reagents
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IFN Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5ug/ml as capture antibody | ||
| ELISpot | |||
| Flow Cytometry 1 | 1/50 | 1/100 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- ELISA
- Mouse anti Bovine interferon-γ antibody, clone CC330 may be used as a capture reagent in a sandwich ELISA in combination with Mouse anti Bovine interferon-γ antibody, clone CC302 (MCA1783B) as a detection reagent and recombinant bovine interferon-γ (PBP007A) as a protein standard.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for IFN Gamma antibody
-
Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.
J Immunol. 169 (7): 3837-46. -
Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24. -
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41 (1): 4. -
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. -
Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. -
Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.
Vet Res. 2009 40: 54. -
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. -
Begg, D.J. et al. (2009) Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease.
J Vet Diagn Invest. 21: 187-96. -
Tourais-Esteves, I. et al. (2008) Neonatal goats display a stronger TH1-type cytokine response to TLR ligands than adults
Dev Comp Immunol. 32: 1231-41. -
Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
J Immunol. 180: 5963-72. -
McLaughlin, K. et al. (2010) Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro.
Vet Res. 41: 36. -
Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
Parasite Immunol. 31: 72-7. -
Baszler, T.V. et al. (2008) Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites.
Clin Vaccine Immunol. 15: 659-67. -
Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
PLoS One. 6:e21978. -
Appana, G. et al. (2013) Antemortem and postmortem examinations of the cattle calf naturally infected with Mycobacterium avium subsp. paratuberculosis.
Eur J Microbiol and Immunol. 3: 241–51. -
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293. -
Köhler H et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.
BMC Vet Res. 11 (1): 74. -
Bergmann, A. et al. (2015) In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.
PLoS One. 10 (4): e0123980. -
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]
