IFN Gamma antibody | CC330
Mouse anti Bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, a 143 amino acid ~20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading miro-organisms. Interferon γ enhances cytotoxic activities of many cell types including macrophages, NK cells and T lymphocytes (Weiss et al. 2002).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep Goat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Recombinant bovine IFN-gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P07353 Related reagents
- Entrez Gene
- IFNG Related reagents
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- For research purposes only
Applications of IFN Gamma antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|ELISA||5ug/ml as capture antibody|
|Flow Cytometry 1||1/50||1/100|
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Mouse anti Bovine interferon-γ antibody, clone CC330 may be used as a capture reagent in a sandwich ELISA in combination with Mouse anti Bovine interferon-γ antibody, clone CC302 (MCA1783B) as a detection reagent and recombinant bovine interferon-γ (PBP007A) as a protein standard.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||100 Tests||F|
Product Specific References
References for IFN Gamma antibody
Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.
J Immunol. 169 (7): 3837-46.
Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41 (1): 4.
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19.
Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25.
Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.
Vet Res. 2009 40: 54.
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705.
Begg, D.J. et al. (2009) Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease.
J Vet Diagn Invest. 21: 187-96.
Tourais-Esteves, I. et al. (2008) Neonatal goats display a stronger TH1-type cytokine response to TLR ligands than adults
Dev Comp Immunol. 32: 1231-41.
Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
J Immunol. 180: 5963-72.
McLaughlin, K. et al. (2010) Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro.
Vet Res. 41: 36.
Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
Parasite Immunol. 31: 72-7.
Baszler, T.V. et al. (2008) Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites.
Clin Vaccine Immunol. 15: 659-67.
Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
PLoS One. 6:e21978.
Appana, G. et al. (2013) Antemortem and postmortem examinations of the cattle calf naturally infected with Mycobacterium avium subsp. paratuberculosis.
Eur J Microbiol and Immunol. 3: 241–51.
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293.
Köhler H et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.
BMC Vet Res. 11 (1): 74.
Bergmann, A. et al. (2015) In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.
PLoS One. 10 (4): e0123980.
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]