IFN Gamma antibody | CC330

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Mouse anti Bovine Interferon Gamma

Product Type
Monoclonal Antibody
Clone
CC330
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2112 E ES F* 0.5 mg
Mouse anti Bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, a 143 amino acid ~20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading miro-organisms. Interferon γ enhances cytotoxic activities of many cell types including macrophages, NK cells and T lymphocytes (Weiss et al. 2002).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant bovine IFN-gamma.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07353 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml as capture antibody
ELISpot
Flow Cytometry 1 1/50 1/100
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
ELISA
Mouse anti Bovine interferon-γ antibody, clone CC330 may be used as a capture reagent in a sandwich ELISA in combination with Mouse anti Bovine interferon-γ antibody, clone CC302 (MCA1783B) as a detection reagent and recombinant bovine interferon-γ (PBP007A) as a protein standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

ELISA

ELISpot

Product Specific References

References for IFN Gamma antibody

  1. Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.
    J Immunol. 169 (7): 3837-46.
  2. Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
    Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
  3. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
    Vet Res. 41 (1): 4.
  4. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  5. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
    J Immunol. 185: 3313-25.
  6. Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.
    Vet Res. 2009 40: 54.
  7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  8. Begg, D.J. et al. (2009) Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease.
    J Vet Diagn Invest. 21: 187-96.
  9. Tourais-Esteves, I. et al. (2008) Neonatal goats display a stronger TH1-type cytokine response to TLR ligands than adults
    Dev Comp Immunol. 32: 1231-41.
  10. Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
    J Immunol. 180: 5963-72.
  11. McLaughlin, K. et al. (2010) Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro.
    Vet Res. 41: 36.
  12. Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
    Parasite Immunol. 31: 72-7.
  13. Baszler, T.V. et al. (2008) Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites.
    Clin Vaccine Immunol. 15: 659-67.
  14. Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
    PLoS One. 6:e21978.
  15. Appana, G. et al. (2013) Antemortem and postmortem examinations of the cattle calf naturally infected with Mycobacterium avium subsp. paratuberculosis.
    Eur J Microbiol and Immunol. 3: 241–51.
  16. Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
    BMC Vet Res. 10: 293.
  17. Köhler H et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.
    BMC Vet Res. 11 (1): 74.
  18. Bergmann, A. et al. (2015) In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.
    PLoS One. 10 (4): e0123980.
  19. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
    Infect Immun. 85 (2): pii: e00845-16. [Epub ahead of print]