IFN Gamma antibody | CC330
IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer’s Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.
From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E.
Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 2014 Dec 16;10(1):293.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
γδ T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5×104 CFUs of B. abortus 2308, and two weeks later γδ T cells (>95% purity) and an enriched TCRαβ (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean ± SD is shown; * P<0.05 versus the enriched TCRαβ cells. Results are representative of two independent experiments.
From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011)
Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections.
PLoS ONE 6(7): e21978.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
γδ T cells require TNF-α to protect against B. abortus infection. C57BL/6 mice treated with anti-TCRγδ mAb or hamster IgG on day -1 and day 3 post-infection with 5×104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-α on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean ± SEM of 10 mice/group is depicted; *P<0.05 versus hamster IgG-treated C57BL/6 mice. Results are from two independent experiments. C. and D. Splenocytes (5×106/ml) from 4 or 7 day B. abortus-infected mice depleted or not of γδ T cells and/or TNF-α or splenocytes from naive C57BL/6 mice were left unstimulated and cultured for 3 days at 37°C/5%CO2; supernatants were harvested for C. TNF-α- or D. IFN-γ- specific ELISA. The mean ± SD of triplicate wells is shown. The results from 7 dpi are representative of two independent experiments. NS = not significant. ND = cytokine production from uninfected mice neutralized of γδ T cells was not determined. * P<0.05 as compared to mice not depleted of γδ T cells within the same TNF-α treatment group at the same time point. † P<0.05 as compared to C57BL/6 mice treated with IgG only at the same time point.
From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Image caption:
Bovine γδ T cells impair B. abortus replication in autologous macrophages via IFN-γ. A.–F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous γδ T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P<0.05 versus colonization by cultured macrophages only from the same animal. B.,D.,F. IFN-γ levels were determined in cell culture supernatants. At 72 and 120 h post-infection, macrophage plus γδ T cell co-cultures from each calf contained more IFN-γ than from macrophages cultured alone. G. NK1.1+ cell-depleted Rag-1−/− mice (4–5 per group) received PBS, bovine macrophages (5×105/mouse), bovine macrophages (5×105/mouse) plus autologous γδ T cells (1×107/mouse), or bovine macrophages plus autologous CD4+ T cells (1×107/mouse) i.p. one day prior to infection with 1×104 CFUs of B. abortus. All cells were derived from Calf #1. On day 7, splenic colonization was determined. Depicted is the mean ± SD; *P<0.05 versus mice given PBS or macrophages only.
From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti bovine interferon gamma antibody, clone CC330 used for the measurement of the gamma interferon response to M. avium infection in a caprine model by ELISA in conjunction with biotinylated Mouse anti Bovine interferon γ antibody, clone CC302 as a detection reagent.
Image caption:
Time course and intensity of IFN-γ response (B) of inoculated and control goats. Box and Whisker Plot represents median value, 25% and 75% percentiles (box), range, outlier values (◯), and extreme values (*). Different letters indicate significant differences between groups (Mann–Whitney-U test, P ≤ 0.05): a – V1 vs. V2, b – V1 vs. V3, c – V1 vs. V4, d – V2 vs. V3, e – V2 vs. V4, f – V3 vs. V4.
From: Köhler H, Soschinka A, Meyer M, Kather A, Reinhold P, Liebler-Tenorio E.
Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.
BMC Vet Res. 2015 Mar 24;11(1):74.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine interferon γ antibody, clone CC330 (MCA2112) used as a capture antibody for the evaluation of interferon secretion by sheep and bovine lymphocytes by ELISpot assay.
Image caption:
C) Representative images (I) and quantification (II) of secreted IFNγ assessed by ELISpot following coincubation of human (n=3), pig (n=3), cattle (n=4) and sheep (n=3) PBMC with medium, 1 μM Ac-6-FP, 1 μM 5-OP-RU or 4 μg/ml ConA for 18 h. Mean SCF/106 cells ± SEM are shown.
From: Edmans MD, Connelley TK, Morgan S, Pediongco TJ, Jayaraman S, Juno JA, Meehan BS, Dewar PM, Maze EA, Roos EO, Paudyal B, Mak JY, Liu L, Fairlie DP, Wang H, Corbett AJ, McCluskey J, Benedictus L, Tchilian E, Klenerman P, Eckle SB. MAIT cell-MR1 reactivity is highly conserved across multiple divergent species.
J Biol Chem. 2024 May 3:107338 [Epub ahead of print].
doi: 10.1016/j.jbc.2024.107338.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine Interferon Gamma
- Product Type
- Monoclonal Antibody
- Clone
- CC330
- Isotype
- IgG1
- Specificity
- IFN Gamma
Antibody Quality and Performance Guarantee
| Mouse anti Bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, also known as immune interferon. Interferon γ is a 143 amino acid ~20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading microorganisms. Interferon γ enhances cytotoxic activities of many cell types including macrophages, NK cells and T lymphocytes (Weiss et al. 2002). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant bovine IFN-gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5μg/ml as capture antibody | |
| ELISpot | |
||
| Flow Cytometry 1 | |
1/50 | 1/100 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
- ELISA
- Mouse anti Bovine interferon-γ antibody, clone CC330 may be used as a capture reagent in a sandwich ELISA in combination with Mouse anti Bovine interferon-γ antibody, clone CC302 (MCA1783B) as a detection reagent and recombinant bovine interferon-γ (PBP007A) as a protein standard.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for IFN Gamma antibody
-
Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.
J Immunol. 169 (7): 3837-46. -
Baszler, T.V. et al. (2008) Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites.
Clin Vaccine Immunol. 15: 659-67. -
Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph.
J Immunol. 180: 5963-72. -
Tourais-Esteves, I. et al. (2008) Neonatal goats display a stronger TH1-type cytokine response to TLR ligands than adults
Dev Comp Immunol. 32: 1231-41. -
Begg, D.J. et al. (2009) Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease.
J Vet Diagn Invest. 21: 187-96. -
Panadero, R. et al. (2009) Immunomodulatory effect of Hypoderma lineatum antigens: in vitro effect on bovine lymphocyte proliferation and cytokine production.
Parasite Immunol. 31: 72-7. -
Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.
Vet Res. 2009 40: 54. -
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705.
View The Latest Product References
-
Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. -
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41 (1): 4. -
McLaughlin, K. et al. (2010) Hsp70 enhances presentation of FMDV antigen to bovine CD4+ T cells in vitro.
Vet Res. 41: 36. -
Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. -
Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24. -
Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
PLoS One. 6:e21978. -
Appana, G. et al. (2013) Antemortem and postmortem examinations of the cattle calf naturally infected with Mycobacterium avium subsp. paratuberculosis.
Eur J Microbiol and Immunol. 3: 241–51. -
Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.
BMC Vet Res. 10: 293. -
Köhler H et al. (2015) Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection.
BMC Vet Res. 11 (1): 74. -
Bergmann, A. et al. (2015) In Vivo Volatile Organic Compound Signatures of Mycobacterium avium subsp. paratuberculosis.
PLoS One. 10 (4): e0123980. -
Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
Infect Immun. 85(2):e00845-16. -
Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
Cytometry A. 91 (9): 901-7. -
Villa-Mancera, A. et al. (2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes of Fasciola hepatica. induces protective cellular and humoral immune responses in sheep.
Vet Parasitol. 289: 109340. -
Risalde, M.Á. et al. (2017) Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis..
BMC Vet Res. 13 (1): 341. -
Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model.
J Dairy Sci. 105 (5): 4335-53. -
Santillo, A. et al. (2022) Feeding tannins to dairy cows in different seasons improves the oxidative status of blood plasma and the antioxidant capacity of cheese.
J Dairy Sci. 105 (11): 8609-20. -
Bouroutzika, E. et al. (2023) Melatonin Administration to Pregnant Ewes for Coccidiosis Control in Their Offspring.
Animals (Basel). 13 (14): 2381. -
Tulu, B. et al. (2020) Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia.
Infect Immun. 88 (12): e00507-20. -
Edmans, M.D. et al. (2024) MAIT cell-MR1 reactivity is highly conserved across multiple divergent species.
J Biol Chem. 107338. 3 May [Epub ahead of print].
- Synonyms
- Immune Interferon
- RRID
- AB_2123456
- UniProt
- P07353
- Entrez Gene
- IFNG
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
View more products with IFN GAMMA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Bovine ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up