IL-2 antibody

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Rabbit anti Human Interleukin-2:Biotin

Rabbit anti Human Interleukin-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1032B
Datasheet
E WB
MSDS
50 µg loader

AHP1032
Datasheet
E FN P* WB
MSDS
0.1 mg loader

Rabbit anti Human Interleukin-2 antibody recognizes human IL-2 (Interleukin-2), otherwise known as T-cell growth factor, a 133 amino acid glycoprotein and potent immunoregulatory cytokine produced by antigen or mitogen stimulated T cells.

IL-2 is a multi-functional cytokine which signals through the IL-2 receptor complex (IL-2R), and is principally involved in the stimulation of the T cell proliferative response, promoting T cell clonal expansion and the expansion of T memory cells expressing low levels of high-affinity IL-2R.

IL-2 also stimulates the growth/differentiation of B cells, monocytes, NK cells, macrophages and lymphokine activated killer cells.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant human IL-2
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstituiton.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of reconstitution.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P60568 Related reagents
Entrez Gene
IL2 Related reagents
GO Terms
GO:0002903 negative regulation of B cell apoptosis
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0019209 kinase activator activity
GO:0030101 natural killer cell activation
GO:0030217 T cell differentiation
GO:0030307 positive regulation of cell growth
GO:0030890 positive regulation of B cell proliferation
GO:0032740 positive regulation of interleukin-17 production
GO:0034105 positive regulation of tissue remodeling
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0050729 positive regulation of inflammatory response
Regulatory
For research purposes only

Applications of IL-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 0.12 0.16ug/ml
Immunohistology - Paraffin 1
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human IL-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-2 antibody (AHP1032) and recombinant human IL-2 (PHP042) as the standard.
ELISA
This purified human IL-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IL-2 antibody (AHP1032B) as the detection reagent and recombinant human IL-2 (PHP042) as the standard

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Human Interleukin-2 AHP1032 E FN P* WB 0.1 mg loader
Rabbit anti Human Interleukin-2:Biotin AHP1032B E WB 50 µg loader
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Positive Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Recombinant Human Interleukin-2 PHP042A E FN WB 10 µg loader
Recombinant Human Interleukin-2 PHP042A E FN WB 10 µg loader

Product Specific References

Further Reading

  1. Fujita, T. et al. (1983) Structure of the human interleukin 2 gene.
    Proc Natl Acad Sci U S A. 80 (24): 7437-41.
  2. Nelson, B.H. (2004) IL-2, regulatory T cells, and tolerance.
    J Immunol. 172 (7): 3983-8.