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Myeloperoxidase antibody | D08-8G5

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Sandwich ELISA analysis of human MPO using Mouse anti Human myeloperoxidase (MCA6260GA) as a capture reagent and biotinylated Mouse anti Human myeloperoxidase (MCA6261B) as a detection reagent with purified native MPO as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

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Mouse anti Human Myeloperoxidase:Biotin

Product Type
Monoclonal Antibody
Clone
D08-8G5
Isotype
IgG1
Specificity
Myeloperoxidase

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Product Code Applications Pack Size List Price Your Price Qty
MCA6261B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human myeloperoxidase antibody, clone D08-8G5, recognizes myeloperoxidase (MPO), a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is largely expressed by neutrophils within azurophilic granules, but is also present in monocytes, macrophages and central nervous microglial cells (Strzepa et al. 2017). MPO is synthesized as a precursor protein that undergoes a series of modifications including cleavage and glycosylation to form the catalytically inactive MPO precursor. Subsequently an iron-heme is incorporated into the protein resulting in the catalytically active MPO (Hansson et al. 2006).

In response to bacterial infection, neutrophils containing MPO fuse with the phagosome and when the common membrane is ruptured, MPO is release into the phagosome. MPO then uses H2O2 to generate the bactericidal compound, hypochlorous acid (HClO) (Strzepa et al. 2017).

MPO deficiency is associated with a higher occurrence of severe infections and chronic inflammatory processes, although ~50% of individuals are asymptomatic (Antachopoulos 2010).

The biotinylated Mouse anti Human myeloperoxidase antibody, clone D08-8G5 (MCA6261B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human myeloperoxidase antibody, clone D02-2A1 (MCA6260GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Purified native human myeloperoxidase
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6260GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Myeloperoxidase MCA6260GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human Myeloperoxidase

UniProt
P05164
Entrez Gene
MPO
GO Terms
GO:0003682 chromatin binding
GO:0006952 defense response
GO:0005764 lysosome
GO:0004601 peroxidase activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0030141 stored secretory granule
GO:0055114 oxidation-reduction process
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0020037 heme binding
GO:0034374 low-density lipoprotein particle remodeling
GO:0042744 hydrogen peroxide catabolic process

MCA6261B

100005434 64594284

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