MMP-2 antibody | C12-8A3

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Mouse anti Human MMP-2

Product Type
Monoclonal Antibody
Clone
C12-8A3
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6256GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human MMP-2 antibody, clone C12-8A3, recognizes matrix metalloproteinase-2 also known as 72 kDa type IV collagenase, 72 kDa gelatinase and gelatinase A. MMP-2 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells, adventitia platelets and leukocytes (Cui et al. 2017).

MMP-2 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 72 kDa pro-MMP-2. Pro-MMP-2 is then recruited to the cell surface where it undergoes autocatalytic cleavage to form the active 63 kDa form.

Like other MMP’s, MMP-2 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-2 degrades the collagen proteins, type I, II, III, IV, V, VII, X, XI and gelatin. It is also able to target other ECM substrates including aggrecan, elastin, fibronectin and laminin (Cui et al. 2017). However a number of non-matrix proteins have been identified as targets of MMP-2, to regulate various physiological and pathophysiological processes including embryonic growth/development, angiogenesis, vascular diseases, inflammation, infective diseases and tumor progression. MMP-2 may also play a role in airway remodeling and may be responsible for the progressive decline of lung function in bronchial asthma (Kuwabara et al. 2018).

The purified Mouse anti Human MMP-2 antibody, clone C12-8A3 (MCA6256GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human MMP-2 antibody, clone J08-3B10 (MCA6257B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human partial length of MMP-2 (Tyr110-Thr465)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P08253
Entrez Gene
MMP2
GO Terms
GO:0005515 protein binding
GO:0001525 angiogenesis
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
Regulatory
For research purposes only

Applications of MMP-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human MMP-2:Biotin MCA6257B E 0.1 mg loader

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