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G-CSF antibody

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Anti Human G-CSF Antibody thumbnail image 1 Anti Human G-CSF Antibody thumbnail image 2
Anti Human G-CSF Antibody gallery image 1
Staining of formalin fixed, paraffin embedded human angiosarcoma with Rabbit anti Human G-CSF (AHP1031) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Anti Human G-CSF Antibody gallery image 2
Staining of formalin fixed, paraffin embedded human angiosarcoma with Rabbit anti Human G-CSF (AHP1031) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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Rabbit anti Human G-CSF:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
G-CSF

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AHP1031B
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Rabbit anti Human G-CSF antibody recognizes human G-CSF (Granulocyte colony-stimulating factor), a 19.6kDa haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage.

The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy.

To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human G-CSF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human G-CSF antibody (AHP1031) as the capture reagent and recombinant human G-CSF (PHP082B) as the standard.

Further Reading

  1. Hill, C.P. et al. (1993) The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.
    Proc Natl Acad Sci U S A. 90 (11): 5167-71.
  2. Morton, J. et al. (2001) Granulocyte-colony-stimulating factor (G-CSF)-primed allogeneic bone marrow: significantly less graft-versus-host disease and comparable engraftment to G-CSF-mobilized peripheral blood stem cells.
    Blood. 98 (12): 3186-91.

RRID
AB_2083735
UniProt
P09919
Entrez Gene
CSF3
GO Terms
GO:0005125 cytokine activity
GO:0005130 granulocyte colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0019899 enzyme binding
GO:0019221 cytokine-mediated signaling pathway
GO:0030851 granulocyte differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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