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|Rabbit anti Human G-CSF antibody recognizes human G-CSF (Granulocyte colony-stimulating factor), a 19.6kDa haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage.
The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy.
To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required.
- Target Species
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antisera to human G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant human G-CSF
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
- For research purposes only
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin|
Hill, C.P. et al. (1993) The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.
Proc Natl Acad Sci U S A. 90 (11): 5167-71.
Morton, J. et al. (2001) Granulocyte-colony-stimulating factor (G-CSF)-primed allogeneic bone marrow: significantly less graft-versus-host disease and comparable engraftment to G-CSF-mobilized peripheral blood stem cells.
Blood. 98 (12): 3186-91.
- Entrez Gene
- GO Terms
- GO:0005125 cytokine activity
- GO:0005130 granulocyte colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0019899 enzyme binding
- GO:0019221 cytokine-mediated signaling pathway
- GO:0030851 granulocyte differentiation
- View More GO Terms
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