IFN Beta antibody | F03-2G4

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Mouse anti Human IFN Beta

Product Type
Monoclonal Antibody
Clone
F03-2G4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6236GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human IFN beta antibody, clone F03-2G4, recognizes interferon beta (IFN beta) also known as fibroblast interferon, a type I interferon. It is constitutively expressed, but is synthesized at a higher level upon infection (Takaoka & Taniguchi 2003). IFN beta plays many roles within inflammation including limiting the immune response during inflammation through induction of IL-10 and can inhibit the inflammasome and IL-1 beta. It can also play a role in the reprogramming of macrophages to an inflammatory and pro-resolving phenotype (Satyanarayanan et al. 2019). These responses are mediated through its interaction with the IFN alpha/beta receptor with subsequent downstream activation of STAT1, 2 or 3 (Takaoka & Taniguchi 2003).

Beyond inflammation IFN beta has been observed to block cancer progression by reducing the recruitment of pro-angiogenic neutrophils into tumors (Andzinski et al. 2015).

The purified Mouse anti Human IFN beta antibody, clone F03-2G4 (MCA6236GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human IFN beta antibody, clone C06-4F6 (MCA6237B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length IFN beta (Met22-Asn187)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01574
Entrez Gene
IFNB1
GO Terms
GO:0003714 transcription corepressor activity
GO:0007596 blood coagulation
GO:0005125 cytokine activity
GO:0005132 interferon-alpha/beta receptor binding
GO:0005615 extracellular space
GO:0006917 induction of apoptosis
GO:0006919 activation of caspase activity
GO:0008285 negative regulation of cell proliferation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030101 natural killer cell activation
GO:0032897 negative regulation of viral transcription
GO:0042100 B cell proliferation
GO:0045071 negative regulation of viral genome replication
GO:0045089 positive regulation of innate immune response
GO:0045343 regulation of MHC class I biosynthetic process
GO:0046597 negative regulation of virion penetration into host cell
GO:0051607 defense response to virus
GO:0060337 type I interferon-mediated signaling pathway
GO:0060338 regulation of type I interferon-mediated signaling pathway
GO:0071360 cellular response to exogenous dsRNA
Regulatory
For research purposes only

Applications of IFN Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human IFN Beta:Biotin MCA6237B E 0.1 mg loader

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