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|Rabbit anti Mouse MIP-2 antibody recognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.
MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Antiserum Preparation
- Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant mouse MIP-2
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- For research purposes only
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Biotinylated Rabbit anti Mouse MIP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM48 as the capture antibody and PMP55 as the standard.
- Western Blotting
- Biotinylated Rabbit anti Mouse MIP-2 antibody may used under either reducing or non-reducing conditions with PMP55 as the positive control.
References for MIP-2 antibody
Tittel, A.P. et al. (2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
J Am Soc Nephrol. 22 (8): 1435-41.
Roche, J.K. et al. (2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.
Am J Pathol. 170: 526-37.
Di Carlo, E. et al. (2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.
J Immunol. 165: 3111-8.
Cavallo, F. et al (2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.
Cancer Res. 61: 3518-23.
González-López, A. et al. (2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.
Am J Physiol Lung Cell Mol Physiol. 301: L500-9.
Lazic, M. et al. (2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.
J Hepatol. 61: 107-15.
Nicholas, J. et al. (2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.
Innate Immun. 21 (3): 266-74.
Yoshida, T. et al. (2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Genes Cells. 19: 842-52.
View The Latest Product References
Lasarte, S. et al. (2016) Sex Hormones Coordinate Neutrophil Immunity in the Vagina by Controlling Chemokine Gradients.
J Infect Dis. 213 (3): 476-84.
Salinas-Muñoz, L. et al. (2019) Estradiol impairs epithelial CXCL1 gradient in the cervix to delay neutrophil transepithelial migration during insemination.
J Reprod Immunol. 132: 9-15.
Konishi, T. et al. (2019) Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
Am J Physiol Gastrointest Liver Physiol. 317 (6): G773-G783.
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