IgG1 antibody | LO-MG1-2

Rat anti Mouse IgG1 Heavy Chain:HRP

Product Type
Monoclonal Antibody
Clone
LO-MG1-2
Isotype
IgG1
Specificity
IgG1
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA336P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 recognizes murine IgG1, and does not bind other mouse immunoglobulin classes or subclasses.

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Immunogen
Purified mouse IgG1 from BALB/c mice
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using approriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with MCA1289 as the capture reagent.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG1 antibody

  1. Song, J. et al. (2000) Heterogeneous distribution of isoactins in cultured vascular smooth muscle cells does not reflect segregation of contractile and cytoskeletal domains.
    J Histochem Cytochem. 48 (11): 1441-52.
  2. Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
    Int Immunol. 5 (1): 71-8.
  3. Nakanishi, S. et al. (2010) Sequence analysis of a bacteriocinogenic plasmid of Clostridium butyricum and expression of the bacteriocin gene in Escherichia coli.

    Anaerobe. 16: 253-7.
  4. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
    Vaccine. 24: 4102-10.
  5. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.
  6. Agallou, M. et al. (2014) Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Proteins.
    Front Immunol. 5: 268.
  7. Doerfler, P.A. et al. (2015) BAFF Blockade Prevents Anti-Drug Antibody Formation in a Mouse Model of Pompe Disease.
    Clin Immunol. pii: S1521-6616(15)00125-4.
  8. Ramos, J.D.A. et al. (2009) Characterization of Blo t 11 Monoclonal Antibodies with Constant Region Mutations
    Phil Sci Lett. 2(1): 38-48
  9. View The Latest Product References
  10. Blackwell, N.M. & Else, K.J. (2002) A comparison of local and peripheral parasite-specific antibody production in different strains of mice infected with Trichuris muris.
    Parasite Immunol. 24 (4): 203-11.
  11. Hall, G. et al. (2003) Suppression of allergen reactive Th2 mediated responses and pulmonary eosinophilia by intranasal administration of an immunodominant peptide is linked to IL-10 production.
    Vaccine. 21 (5-6): 549-61.
  12. Hjerpe, C. et al. (2010) Dendritic cells pulsed with malondialdehyde modified low density lipoprotein aggravate atherosclerosis in Apoe(-/-) mice.
    Atherosclerosis. 209 (2): 436-41.
  13. Kretschmer, B. et al. (2015) Anti-CD83 promotes IgG1 isotype switch in marginal zone B cells in response to TI-2 antigen.
    Immunobiology. 220 (8): 964-75.
  14. Doerfler, P.A. et al. (2016) Copackaged AAV9 Vectors Promote Simultaneous Immune Tolerance and Phenotypic Correction of Pompe Disease.
    Hum Gene Ther. 27 (1): 43-59.
  15. Kato, G. et al. (2014) β2 adrenergic agonist attenuates house dust mite-induced allergic airway inflammation through dendritic cells.
    BMC Immunol. 15: 39.
  16. Margaroni, M. et al. (2017) Vaccination with poly(D,L-lactide-co-glycolide) nanoparticles loaded with soluble Leishmania antigens and modified with a TNFα-mimicking peptide or monophosphoryl lipid A confers protection against experimental visceral leishmaniasis.
    Int J Nanomedicine. 12: 6169-84.
  17. DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
    Cancers (Basel). 11 (4) Apr 11 [Epub ahead of print].
  18. Doshi, B.S. et al. (2021) B cell-activating factor modulates the factor VIII immune response in hemophilia A.
    J Clin Invest. 131(8):142906.
  19. Scaramozza, A. et al. (2019) Lineage Tracing Reveals a Subset of Reserve Muscle Stem Cells Capable of Clonal Expansion under Stress.
    Cell Stem Cell. 24 (6): 944-957.e5.
  20. Steel, N. et al. (2019) TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
    PLoS Pathog. 15 (4): e1007657.
  21. Salem, E. et al. (2019) Pathogenesis, Host Innate Immune Response, and Aerosol Transmission of Influenza D Virus in Cattle.
    J Virol.93(7):e01853-18.

ELISA

RRID
AB_321957
UniProt
P01869
P01868
Entrez Gene
Ighg1
Ighg1
GO Terms
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0003823 antigen binding
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0005886 plasma membrane
GO:0016021 integral to membrane
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products