IL-13 antibody
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Rabbit anti Mouse Interleukin-13 antibody recognizes mouse Interleukin-13 (IL-13), a 12.3kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells and also by mast cells and NK cells.
The biological properties of IL-13 include stimulation of B cell proliferation and antibody production and studies suggest that IL-13 plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E and eosinophils (Wills-Karp et al. 1998).
The biological properties of IL-13 include stimulation of B cell proliferation and antibody production and studies suggest that IL-13 plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E and eosinophils (Wills-Karp et al. 1998).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to mouse IL-13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant mouse IL-13.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20109
- Entrez Gene
- Il13
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0043032 positive regulation of macrophage activation
- Regulatory
- For research purposes only
Applications of IL-13 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5 | 2.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Mouse Interleukin-13 | AAM34 | E WB | 0.1 mg |
|
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| Rabbit anti Mouse Interleukin-13:Biotin | AAM34B | E WB | 50 µg |
|
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IL-13 antibody
-
Wills-karp, M. et al. (1998) Interleukin-13: central mediator of allergic asthma.
Science. 282 (5397): 2258-61.
