VEGF antibody

Goat anti Human VEGF:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
VEGF

Product Code Applications Pack Size List Price Your Price Qty
AHP831B
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SDS Safety Datasheet SDS
E WB 50 µg loader
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Goat anti Human VEGF antibody recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.

The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human VEGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human VEGF antibody (AHP831) as the capture reagent and recombinant human VEGF (PHP091) as the standard.
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF (PHP091) as the positive control.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human VEGF AHP831 E FN P* WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Human VEGF

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human VEGF PHP091 E FN WB 10 µg loader
List Price Your Price
loader
Description Recombinant Human VEGF

References for VEGF antibody

  1. Hjelmgren, O. et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
    PLoS One. 11 (12): e0166918.
  2. Gamliel, M. et al. (2018) Trained Memory of Human Uterine NK Cells Enhances Their Function in Subsequent Pregnancies.
    Immunity. 48 (5): 951-962.e5.

Further Reading

  1. Neufeld, G. et al. (1999) Vascular endothelial growth factor (VEGF) and its receptors.
    FASEB J. 13: 9-22.

RRID
AB_2212844
UniProt
P15692
Entrez Gene
VEGFA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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