StarBright Violet Dyes

Excitable by the 405 nm laser, with a broad range of maximal emissions from 440 nm to 790 nm, StarBright Violet (SBV) Dyes are designed to be the ideal choice when building multicolor panels using the 405 laser in conventional and full spectrum flow cytometry. They are bright, photostable, compatible with most buffers, and have narrow excitation and emission spectra. Table 1 below shows all the available SBV Dyes.

Visit StarBright Dyes to discover all the benefits of these dyes.

SBV Dye Range

StarBright Dye

Max Ex, nm

Max Em, nm

Brightness (1–5)

ZE5 Cell Analyzer Optimal Filter

Comparison Dye

Launch Date

Flier

StarBright Violet 440 Dye

383

436

5

460/22

Super Bright (SB) 436 , Pacific Blue, eFluor 450

Available now

SBV440 PDF

StarBright Violet 475  Dye

405

479

4

525/50

Brilliant Violet (BV) 480

Available now

SBV475 PDF

StarBright Violet 515  Dye

402

516

5

525/50

BV510, Amethyst Orange

Available now

SBV515 PDF

StarBright Violet 570  Dye

402

570

4

615/24

BV570

Available now

SBV570 PDF

StarBright Violet 610  Dye

402

607

5

615/24

BV605, SB600

Available now

SBV610 PDF

StarBright Violet 670  Dye

401

667

5

670/30

BV650, SB645

Available now

SBV670 PDF

StarBright Violet 710  Dye

402

713

5

720/60

BV711, SB702

Available now

SBV710 PDF

StarBright Violet 760  Dye

403

760

5

750LP

BV750

Coming soon

Coming soon

StarBright Violet 790  Dye

402

782

4

750LP

BV785, SB780

Available now

SBV790 PDF


Fig. 1. Excitation and emission spectra of StarBright Violet Dyes.


Fig. 1. Excitation and emission spectra of StarBright Violet Dyes.


StarBright Violet Dyes in Multicolor Panels

StarBright Dyes have been designed to be bright with narrow excitation and emission spectra and are therefore an excellent choice for use in multicolor panels, as demonstrated in Figures 2 and 3. Human peripheral blood was stained with a 12-color panel and mouse splenocytes were stained in a seven-color panel, both containing six StarBright Violet Dyes and other commonly used dyes such as Alexa Fluors, FITC, PE, and PE-tandem dyes. Figure 2 and Figure 3 show the human and mouse data and Tables 1 and 2 the antibodies used in each panel.

Fig. 2. Human peripheral blood was stained with antibodies conjugated to StarBright Violet Dyes, to identify major lineages and subsets.


Fig. 2. Human peripheral blood was stained with antibodies conjugated to StarBright Violet Dyes, to identify major lineages and subsets. T cells, B cells, and myeloid lineages could easily be separated in this panel. Within the T cell compartment, T helper and T cytotoxic cells could be identified. Further, the memory, naïve, and effector memory status of these subsets could be detected based on CD45RA, CD45RO, CD27, and CD57 expression. Classical, nonclassical, and intermediate monocytes could be found using CD14 and CD16. Data were analyzed in FCS Express Software.

Table 1. Fluorescent dyes used in StarBright Violet Dye 12-color human immunophenotyping panel.

 Marker

Catalog #

Dyes

CD3

MCA463SBV440

SBV440

CD4

MCA1267SBV610

SBV610

CD8

MCA1226SBV710

SBV710

CD14

MCA1568SBV515

SBV515

CD19

MCA1940SBV670

SBV670

CD45

MCA87P647

PE-A647

L/D

1351111

VivaFix 355/442

CD57

MCA1305F

FITC

CD45RA

MCA88A700

A700

CD45RO

MCA461SBV790

SBV790

CD16

MCA2357PE

PE

CD27

MCA755A647

A647


Fig. 3. Murine splenocytes were stained with antibodies conjugated to StarBright Violet Dyes, to identify major lineages and subsets.


Fig. 3. Murine splenocytes were stained with antibodies conjugated to StarBright Violet Dyes, to identify major lineages and subsets. T, B, and NK cells as well as myeloid lineages could easily be separated in this panel. Within the T cell compartment, T helper and T cytotoxic cells could be identified. Data were analyzed in FCS Express Software.

Table 2. Fluorescent dyes used in StarBright Violet Dye seven-color mouse immunophenotyping panel.

 Marker

Catalog #

Dyes

CD3

MCA500SBV440

SBV440

CD4

MCA2691SBV610

SBV610

CD8

MCA609SBV710

SBV710

CD11b

MCA711SBV515

SBV515

CD45R

MCA1258SBV670

SBV670

CD161

MCA1266SBV790

SBV790

L/D

1351111

VivaFix 355/442

The same human and mouse panels containing Brilliant Violet or Super Bright Dyes were directly compared with the StarBright Dye panel and the quality of staining assessed. In addition to having increased separation of the positive and negative populations, there was reduced spillover and spreading in the panels containing StarBright Violet Dye compared with the panels containing Brilliant Violet and Super Bright Dyes.


Superior Staining Seen with StarBright Violet Dyes

Although all three human immunophenotyping panels allowed the identification of the major lineages, there were significant improvements in the separation of populations when StarBright Violet (SBV) Dyes were used. The superior brightness of SBV Dyes gives improved resolution with the added benefit of reduced spreading due to the narrow excitation and emission profiles. Furthermore, as no special buffer is required, this staining was done in PBS 1% BSA.

Fig. 4. StarBright Violet Dye stained panels show improvements over panels containing other dyes.


Fig. 4. StarBright Violet Dye stained panels show improvements over panels containing other dyes. The increased brightness of StarBright Dyes compared to Brilliant Violet and Super Bright dyes can be clearly seen in the CD19+ B cells, CD14+ monocytes, and CD8+ T cytotoxic cells. Furthermore, the separation of CD45RA and CD45RO expression to determine naïve and memory phenotypes in the CD4 positive population is improved.

StarBright Violet Dyes are also suitable for use in full spectrum flow cytometry. Visit our Spectral Flow Cytometry with StarBright Dyes page for more information on performance and novel combinations that can help you build bigger, better panels.

StarBright Dyes are also available in blue (488 nm excitable) and ultraviolet (355 nm excitable). Click on the links below to find out more about the dyes excited by other laser lines.


StarBright Violet Dye Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    Associated Reading

    For more information on how these products can improve the effectiveness of your flow cytometry experiments, please take a look at our Starbright Dyes page.

    Useful information about fluorescent dyes and immunophenotyping can be found in our flow cytometry explained knowledge hub. Alternatively, feel free to explore the associated resource links below, for more in-depth information about each cited topic. If you are interested in learning the fundamentals of this application, please view our flow cytometry basics guide.