Immunoglobulins (Ig), commonly known as antibodies, are secreted by plasma cells in response to the presence of immunogen and have a role in the fight against infection. There are five isotypes IgA, IgD, IgE, IgG and IgM in placental mammals and another isotype IgY can be found in the egg yolk of birds.
In immunoassays and biochemical applications, purified Igs are widely used as positive or negative controls, standards, blocking reagents or secondary antibodies. Highly purified antibodies can be used to decrease the non-specific background signals in immunoenzyme analysis, and as secondary antibodies in immunoblotting assays.
They may also serve as material for the preparation of immunogens (i.e., IgG, IgM, IgA, IgE, IgD, immunoglobulin λ and κ chains are commonly used as immunogens for the production of antiserum), solid-phase immunosorbents for the binding of cross reactive antibodies, and the purification of anti-immunoglobulin antibodies from polyclonal sera.
Bio-Rad offers a broad range of highly purified native immunoglobulins from various species in different isotypes and subclasses, available in conjugated and non-conjugated form. We also produce a comprehensive list of fully-human recombinant immunoglobulins for use as negative controls in preclinical and in vitro studies.