Our Antibody Validation Principles

“Validation is the experimental proof and documentation that a specific antibody is suitable for an intended application or purpose” (Weller 2018)

With over 35 years’ experience in manufacturing antibodies, adapting to the evolving needs of our customers is at the heart of our business. We know you need results that you can rely on and a consistent performance of your antibody to ensure the reproducibility of your results.  As such, we are committed to evolving our validation standards to ensure quality and reproducibility, and to develop rigorous validation guidelines benefiting the scientific community as a whole.

As Antibody Manufacturers, Product Quality Is Essential
 

Guarantee Bio-Rad conducts rigorous in house testing to guarantee that all of our antibodies meet our internal benchmarks and perform in their designated applications as expected.  Our stringent quality control process is recognized by ISO9001:2015 certification at our manufacturing sites in Kidlington, Oxfordshire, UK, and Puchheim, Germany.

We clearly display recommended applications for every antibody on our website and product datasheet.
In addition, Bio-Rad Quality and Performance Guarantee  ensures that if a catalog antibody batch doesn’t meet the specifications as described on the datasheet, and the issue cannot be resolved by our technical support team, we will replace the antibody free of charge or offer a credit.


Transparency Is Key
 

Transparency Sharing your knowledge can help other scientists find the best antibodies for their research. Your feedback is therefore vital to help other researchers understand how an antibody has performed in different applications and species. Including extensive details of antibodies used in your publications such as vendor, clone ID and experimental conditions will help other scientists verify your findings. These details also help validate that antibody for the published application.

 


We Are Working to Raise Antibody Standards
 

driving change To address the antibody reproducibility crisis (Goodman 2018), Bio-Rad ensures that we stand behind every vial of product we sell. We listen to feedback from the scientific community about how our antibodies perform and constantly review published literature to identify where our antibodies have been demonstrated to work in additional applications. If we receive information about an antibody underperforming, we launch an investigation; withdrawing product from sale where necessary. This ensures that you can be confident our antibodies meet our high standards and give reproducible results.

We appreciate that as an antibody manufacturer we need to help transform the validation landscape. To enable change, Bio-Rad is working alongside other antibody manufacturers and suppliers, as part of The Antibody Consortium, to establish best practices for antibody validation. We are implementing methods to validate our antibodies in accordance with recommendations by the International Working Group for Antibody Validation (Uhlen et al. 2016).


Antibodies Verified for Western Blotting
 

Our PrecisionAb Antibodies are assessed to ensure superior performance and reproducibility in western blotting. Only antibodies meeting all of our stringent criteria are released as PrecisionAb Antibodies.

 

Antibodies Verified for Flow Cytometry
 

In house manufactured antibodies that have ‘flow cytometry’ indicated on the datasheet have been assessed to ensure application suitability. This includes testing the antibody for performance in flow cytometry using a relevant cell line, or primary cells, known to express the marker under appropriate conditions. They also have to meet internal criteria including batch-to-batch consistency for dilution, percentage of positive cells, and shift.


References

  • Goodman SL (2018). The antibody horror show: an introductory guide for the perplexed. New Biotechnology, 45, 9-13.
  • Uhlen M et al. (2016). A proposal for validation of antibodies. Nature Methods, 13, 823-827.
  • Weller MG (2018). Ten basic rules of antibody validation. Anal Chem Insights, 13: 1177390118757462.