Overview

A matched antibody pair for ELISA consists of a capture antibody and a detection antibody. In a matched ELISA pair, each antibody is specific for a different and non-overlapping region or epitope of the antigen. By design, matched pairs function in a sandwich ELISA set-up; for further information on sandwich ELISA, guides and protocols go to the main ELISA page. Figure 1 below shows the sandwich ELISA protocol.

Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.

Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.

We have validated our matched antibody pairs in sandwich ELISA tests, removing any concerns about performance and reagent selection; the assay data can be found on the respective product pages. We also offer protein standards, as controls and for generation of a standard curve, for a subset of the matched ELISA pairs.

View our recommended ELISA protocol for use with matched antibody pairs.

See the tables below for our range of matched ELISA antibody pairs for the following species:


Human

Table 1. Human matched ELISA antibody pairs.

Specificity

Antibody Type

Catalog Number

Description

A2M

Capture

MCA6216GA

Mouse Anti-Human A2M

A2M

Detection

MCA6217B

Mouse Anti-Human A2M:Biotin

Adiponectin

Capture

MCA6174GA

Mouse Anti-Human Adiponectin

Adiponectin

Detection

MCA6175B

Mouse Anti-Human Adiponectin:Biotin

Apolipoprotein A1

Capture

0650-0050

Mouse Anti-Human Apolipoprotein A1

Apolipoprotein A1

Detection

0650-0180

Sheep Anti-Human Apolipoprotein A1

Apolipoprotein A1

Detection

0650-0190

Sheep Anti-Human Apolipoprotein A1:HRP

BDNF

Capture

AHP1831

Rabbit Anti-Human BDNF

BDNF

Detection

AHP1831B

Rabbit Anti-Human BDNF:Biotin

BMP-2

Capture

AHP960

Rabbit Anti-Human BMP-2

BMP-2

Detection

AHP960B

Rabbit Anti-Human BMP-2:Biotin

CCL2

Capture

MCA6218GA

Mouse Anti-Human CCL2

CCL2

Detection

MCA6219B

Mouse Anti-Human CCL2:Biotin

CCL3

Capture

MCA6220GA

Mouse Anti-Human CCL3

CCL3

Detection

MCA6221B

Mouse Anti-Human CCL3:Biotin

CCL3L1

Capture

AHP1832

Goat Anti-Human CCL3L1

CCL3L1

Detection

AHP1832B

Goat Anti-Human CCL3L1:Biotin

CCL8

Capture

MCA6266GA

Mouse Anti-Human CCL8

CCL8

Detection

MCA6267B

Mouse Anti-Human CCL8:Biotin

CCL13

Capture

MCA6176GA

Mouse Anti-Human CCL13

CCL13

Detection

MCA6177B

Mouse Anti-Human CCL13:Biotin

CCL14

Capture

MCA6178GA

Mouse Anti-Human CCL14

CCL14

Detection

MCA6179B

Mouse Anti-Human CCL14:Biotin

CCL17

Capture

AHP1919

Rabbit Anti-Human CCL17

CCL17

Detection

AHP1919B

Rabbit Anti-Human CCL17:Biotin

CCL17

Capture

MCA6180GA

Mouse Anti-Human CCL17

CCL17

Detection

MCA6181B

Mouse Anti-Human CCL17:Biotin

CCL20

Capture

MCA6202GA

Mouse Anti-Human CCL20

CCL20

Detection

MCA6203B

Mouse Anti-Human CCL20:Biotin

CCL24

Capture

MCA6182GA

Mouse Anti-Human CCL24

CCL24

Detection

MCA6183B

Mouse Anti-Human CCL24:Biotin

CCL27

Capture

MCA6184GA

Mouse Anti-Human CCL27

CCL27

Detection

MCA6185B

Mouse Anti-Human CCL27:Biotin

CD30

Capture

MCA6270GA

Mouse Anti-Human CD30

CD30

Detection

MCA6271B

Mouse Anti-Human CD30:Biotin

CD62P

Capture

MCA6274GA

Mouse Anti-Human CD62P

CD62P

Detection

MCA6275B

Mouse Anti-Human CD62P:Biotin

CD95

Capture

MCA6272GA

Mouse Anti-Human CD95

CD95

Detection

MCA6273B

Mouse Anti-Human CD95:Biotin

CD120b

Capture

AHP1833

Rabbit Anti-Human CD120b

CD120b

Detection

AHP1833B

Rabbit Anti-Human CD120b:Biotin

CD130

Capture

MCA6232GA

Mouse Anti-Human CD130

CD130

Detection

MCA6233B

Mouse Anti-Human CD130:Biotin

CD258

Capture

MCA6280GA

Mouse Anti-Human CD258

CD258

Detection

MCA6281B

Mouse Anti-Human CD258:Biotin

CRP

Capture

MCA6186GA

Mouse Anti-Human CRP

CRP

Detection

MCA6187B

Mouse Anti-Human CRP:Biotin

CTGF

Capture

AHP1278

Rabbit Anti-Human CTGF

CTGF

Detection

AHP1278B

Rabbit Anti-Human CTGF:Biotin

CXCL7

Capture

MCA6224GA

Mouse Anti-Human CXCL7

CXCL7

Detection

MCA6225B

Mouse Anti-Human CXCL7:Biotin

CXCL9

Capture

MCA6252GA

Mouse Anti-Human CXCL9

CXCL9

Detection

MCA6253B

Mouse Anti-Human CXCL9:Biotin

CXCL10

Capture

MCA6188GA

Mouse Anti-Human CXCL10

CXCL10

Detection

MCA6189B

Mouse Anti-Human CXCL10:Biotin

CXCL13

Capture

AHP766

Rabbit Anti-Human CXCL13

CXCL13

Detection

AHP766B

Rabbit Anti-Human CXCL13:Biotin

CXCL16

Capture

MCA6222GA

Mouse Anti-Human CXCL16

CXCL16

Detection

MCA6223B

Mouse Anti-Human CXCL16:Biotin

Cystatin C

Capture

MCA6226GA

Mouse Anti-Human Cystatin C

Cystatin C

Detection

MCA6227B

Mouse Anti-Human Cystatin C:Biotin

Defensin beta 2

Capture

AHP849

Goat Anti-Human Defensin Beta 2

Defensin beta 2

Detection

AHP849B

Goat Anti-Human Defensin Beta 2:Biotin

EGF

Capture

AHP767

Rabbit Anti-Human EGF

EGF

Detection

AHP767B

Rabbit Anti-Human EGF:Biotin

FGF basic

Capture

AHP1038

Rabbit Anti-Human FGF Basic

FGF basic

Detection

AHP1038B

Rabbit Anti-Human FGF Basic:Biotin

Factor H

Capture

MCA6228GA

Mouse Anti-Human Complement Factor H

Factor H

Detection

MCA6229B

Mouse Anti-Human Complement Factor H:Biotin

Fibrinogen

Capture

MCA6190GA

Mouse Anti-Human Fibrinogen

Fibrinogen

Detection

MCA6191B

Mouse Anti-Human Fibrinogen:Biotin

FSH

Capture

0100-0663

Mouse Anti-Human FSH

FSH

Detection

0100-0662

Mouse Anti-Human FSH Alpha

FSH

Detection

0100-0662B

Mouse Anti-Human FSH Alpha:Biotin

G-CSF

Capture

1012701

Rat Anti-Human G-CSF

G-CSF

Detection

1012808

Rat Anti-Human G-CSF:Biotin

G-CSF

Capture

AHP1031

Rabbit Anti-Human G-CSF

G-CSF

Detection

AHP1031B

Rabbit Anti-Human G-CSF:Biotin

Granzyme B

Capture

MCA2120

Mouse Anti-Granzyme B

Granzyme B

Detection

MCA2119B

Mouse Anti-Granzyme B:Biotin

GM-CSF

Capture

MCA6230GA

Mouse Anti-Human GM-CSF

GM-CSF

Detection

MCA6231B

Mouse Anti-Human GM-CSF:Biotin

Haptoglobin

Capture

MCA6234GA

Mouse Anti-Human Haptoglobin

Haptoglobin

Detection

MCA6235B

Mouse Anti-Human Haptoglobin:Biotin

IFN Beta

Capture

MCA6236GA

Mouse Anti-Human IFN Beta

IFN Beta

Detection

MCA6237B

Mouse Anti-Human IFN Beta:Biotin

IFN gamma

Capture

MCA6238GA

Mouse Anti-Human IFN Gamma

IFN gamma

Detection

MCA6239B

Mouse Anti-Human IFN Gamma:Biotin

IFN gamma

Capture

AHP775

Rabbit Anti-Human Interferon Gamma

IFN gamma

Detection

AHP775B

Rabbit Anti-Human Interferon Gamma:Biotin

IFN gamma

Capture

HCA043

Human Anti-Human Interferon Gamma

IFN gamma

Detection

HCA044

Human Anti-Human Interferon Gamma

IGF-I

Capture

AHP1281

Rabbit Anti-Human Insulin-Like Growth Factor I

IGF-I

Detection

AHP1281B

Rabbit Anti-Human Insulin-Like Growth Factor I:Biotin

IL-1RA

Capture

AHP2184

Rabbit Anti-Human Interleukin-1RA

IL-1RA

Detection

AHP2184B

Rabbit Anti-Human Interleukin-1RA:Biotin

IL-2

Capture

AHP1032

Rabbit Anti-Human Interleukin-2

IL-2

Detection

AHP1032B

Rabbit Anti-Human Interleukin-2:Biotin

IL-2

Capture

MCA1552

Rat anti Human Interleukin-2

IL-2

Detection

AHP1032B

Rabbit Anti-Human Interleukin-2:Biotin

IL-4

Capture

MCA6240GA

Mouse Anti-Human Interleukin-4

IL-4

Detection

MCA6241B

Mouse Anti-Human Interleukin-4:Biotin

IL-5

Capture

AHP1034

Rabbit Anti-Human Interleukin-5

IL-5

Detection

AHP1034B

Rabbit Anti-Human Interleukin-5:Biotin

IL-6

Capture

MCA6333GA

Mouse Anti-Human Interleukin-6

IL-6

Detection

MCA6334B

Mouse Anti-Human Interleukin-6:Biotin

IL-6

Capture

1012001

Rat Anti-Human Interleukin-6

IL-6

Detection

1012108

Rat Anti-Human Interleukin-6:Biotin

IL-6

Capture

AHP1040

Rabbit Anti-Human Interleukin-6

IL-6

Detection

AHP1040B

Rabbit Anti-Human Interleukin-6:Biotin

IL-8

Capture

MCA6192GA

Mouse Anti-Human Interleukin-8

IL-8

Detection

MCA6193B

Mouse Anti-Human Interleukin-8:Biotin

IL-11

Capture

MCA6204GA

Mouse Anti-Human Interleukin-11

IL-11

Detection

MCA6205B

Mouse Anti-Human Interleukin-11:Biotin

IL-12 p40

Capture

MCA6194GA

Mouse Anti-Human Interleukin-12p40

IL-12 p40

Detection

MCA6195B

Mouse Anti-Human Interleukin-12p40:Biotin

IL-17A

Capture

MCA6242GA

Mouse Anti-Human Interleukin-17A

IL-17A

Detection

MCA6243B

Mouse Anti-Human Interleukin-17A:Biotin

IL-17A/F

Capture

MCA6206GA

Mouse Anti-Human Interleukin-17A/F

IL-17A/F

Detection

MCA6207B

Mouse Anti-Human Interleukin-17A/F:Biotin

IL-17F

Capture

MCA6208GA

Mouse Anti-Human Interleukin-17F

IL-17F

Detection

MCA6209B

Mouse Anti-Human Interleukin-17F:Biotin

IL-18BP

Capture

MCA6244GA

Mouse Anti-Human Interleukin-18BP

IL-18BP

Detection

MCA6245B

Mouse Anti-Human Interleukin-18BP:Biotin

IL-25

Capture

MCA6248GA

Mouse Anti-Human Interleukin-25

IL-25

Detection

MCA6249B

Mouse Anti-Human Interleukin-25:Biotin

IL-28a

Capture

MCA6210GA

Mouse Anti-Human Interleukin-28a

IL-28a

Detection

MCA6211B

Mouse Anti-Human Interleukin-28a:Biotin

IL-31

Capture

MCA6246GA

Mouse Anti-Human Interleukin-31

IL-31

Detection

MCA6247B

Mouse Anti-Human Interleukin-31:Biotin

IL-32

Capture

MCA6212GA

Mouse Anti-Human Interleukin-32

IL-32

Detection

MCA6213B

Mouse Anti-Human Interleukin-32:Biotin

IL-34

Capture

MCA6214GA

Mouse Anti-Human Interleukin-34

IL-34

Detection

MCA6215B

Mouse Anti-Human Interleukin-34:Biotin

LOX-1

Capture

MCA6250GA

Mouse Anti-Human LOX-1

LOX-1

Detection

MCA6251B

Mouse Anti-Human LOX-1:Biotin

MCP-1

Capture

AHP784

Rabbit Anti-Human MCP-1

MCP-1

Capture

MCA2486

Mouse Anti-Human MCP-1

MCP-1

Detection

AHP784B

Rabbit Anti-Human MCP-1:Biotin

MMP-1

Capture

MCA6254GA

Mouse Anti-Human MMP-1

MMP-1

Detection

MCA6255B

Mouse Anti-Human MMP-1:Biotin

MMP-2

Capture

MCA6256GA

Mouse Anti-Human MMP-2

MMP-2

Detection

MCA6257B

Mouse Anti-Human MMP-2:Biotin

MMP-3

Capture

MCA6258GA

Mouse Anti-Human MMP-3

MMP-3

Detection

MCA6259B

Mouse Anti-Human MMP-3:Biotin

MMP-9

Capture

MCA6196GA

Mouse Anti-Human MMP-9

MMP-9

Detection

MCA6197B

Mouse Anti-Human MMP-9:Biotin

MPO

Capture

MCA6260GA

Mouse Anti-Human Myeloperoxidase

MPO

Detection

MCA6261B

Mouse Anti-Human Myeloperoxidase:Biotin

NGAL

Capture

MCA6198GA

Mouse Anti-Human NGAL

NGAL

Detection

MCA6199B

Mouse Anti-Human NGAL:Biotin

Osteopontin

Capture

MCA6262GA

Mouse Anti-Human Osteopontin

Osteopontin

Detection

MCA6263B

Mouse Anti-Human Osteopontin:Biotin

PDGF-BB

Capture

MCA6264GA

Mouse Anti-Human PDGF-BB

PDGF-BB

Detection

MCA6265B

Mouse Anti-Human PDGF-BB:Biotin

Prolactin

Capture

MCA6200GA

Mouse Anti-Human Prolactin

Prolactin

Detection

MCA6201B

Mouse Anti-Human Prolactin:Biotin

Prolactin

Capture

0100-0665

Mouse Anti-Human Prolactin

Prolactin

Detection

0100-0664

Mouse Anti-Human Prolactin

RAGE

Capture

MCA6276GA

Mouse Anti-Human RAGE

RAGE

Detection

MCA6277B

Mouse Anti-Human RAGE:Biotin

RANTES

Capture

AHP340G

Rabbit Anti-Human RANTES

RANTES

Detection

AHP340B

Rabbit Anti-Human RANTES:Biotin

SAP

Capture

MCA6268GA

Mouse Anti-Human SAP

SAP

Detection

MCA6269B

Mouse Anti-Human SAP:Biotin

SDF-1 alpha

Capture

AHP794

Rabbit Anti-Human SDF-1 Alpha

SDF-1 alpha

Detection

AHP794B

Rabbit Anti-Human SDF-1 Alpha:Biotin

TIMP-1

Capture

MCA6278GA

Mouse Anti-Human TIMP-1

TIMP-1

Detection

MCA6279B

Mouse Anti-Human TIMP-1:Biotin

TNF alpha

Capture

AHP1212

Goat Anti-Human TNF Alpha

TNF alpha

Detection

AHP1212B

Goat Anti-Human TNF Alpha:Biotin

Thyroglobulin

Capture

8900-0700

Mouse Anti-Human Thyroglobulin

Thyroglobulin

Detection

8900-0730

Mouse Anti-Human Thyroglobulin

tPA

Capture

MCA6282GA

Mouse Anti-Human tPA

tPA

Detection

MCA6283B

Mouse Anti-Human tPA:Biotin

TSLP

Capture

MCA6284GA

Mouse Anti-Human TSLP

TSLP

Detection

MCA6285B

Mouse Anti-Human TSLP:Biotin

VEGF

Capture

MCA6286GA

Mouse Anti-Human VEGF

VEGF

Detection

MCA6287B

Mouse Anti-Human VEGF:Biotin

VEGF

Capture

AHP831

Goat Anti-Human VEGF

VEGF

Detection

AHP831B

Goat Anti-Human VEGF:Biotin

vWF

Capture

MCA6288GA

Mouse Anti-Human vWF

vWF

Detection

MCA6289B

Mouse Anti-Human vWF:Biotin

WNT-3a

Capture

AHP2188

Rabbit Anti-Human WNT-3a

WNT-3a

Detection

AHP2188B

Rabbit Anti-Human WNT-3a:Biotin

YKL-40

Capture

MCA6290GA

Mouse Anti-Human YKL-40

YKL-40

Detection

MCA6291B

Mouse Anti-Human YKL-40:Biotin

Mouse

Table 2. Mouse matched ELISA antibody pairs.

Specificity

Antibody Type

Catalog Number

Description

CXCL1

Capture

AAM40

Rabbit Anti-Mouse CXCL1

CXCL1

Detection

AAM40B

Rabbit Anti-Mouse CXCL1:Biotin

IgG1

Capture

MCA1289

Rat Anti-Mouse IgG1

IgG1

Detection

MCA336P

Rat Anti-Mouse IgG1 Heavy Chain:HRP

IgG2a

Capture

MCA1588

Rat Anti-Mouse IgG2a Heavy Chain

IgG2a

Detection

MCA421P

Rat Anti-Mouse IgG2a Heavy Chain:HRP

IgG2b

Capture

MCA1589

Rat Anti-Mouse IgG2b

IgG2b

Detection

MCA1291P

Rat Anti-Mouse IgG2b:HRP

IgG3

Capture

MCA1292

Rat Anti-Mouse IgG3

IgG3

Detection

MCA423B

Rat Anti-Mouse IgG3 Heavy Chain:Biotin

IgM

Capture

MCA1293

Rat Anti-Mouse IgM Heavy Chain

IgM

Detection

MCA199B

Rat Anti-Mouse IgM Heavy Chain:Biotin

IL-1 beta

Capture

AAM13G

Rabbit Anti-Mouse Interleukin-1 Beta

IL-1 beta

Detection

AAM13B

Rabbit Anti-Mouse Interleukin-1 Beta:Biotin

IL-2

Capture

AAM35

Rabbit Anti-Mouse Interleukin-2

IL-2

Detection

AAM35B

Rabbit Anti-Mouse Interleukin-2:Biotin

IL-13

Capture

AAM34

Rabbit Anti-Mouse Interleukin-13

IL-13

Detection

AAM34B

Rabbit Anti-Mouse Interleukin-13:Biotin

IL-21

Capture

AAM61

Rabbit Anti-Mouse Interleukin-21

IL-21

Detection

AAM61B

Rabbit Anti-Mouse Interleukin-21:Biotin

IP-10

Capture

AAM39

Rabbit Anti-Mouse IP-10

IP-10

Detection

AAM39B

Rabbit Anti-Mouse IP-10:Biotin

MIP-2

Capture

AAM48

Rabbit Anti-Mouse MIP-2

MIP-2

Detection

AAM48B

Rabbit Anti-Mouse MIP-2:Biotin

SDF-1 alpha

Capture

AAM64

Goat Anti-Mouse SDF-1 Alpha

SDF-1 alpha

Detection

AAM64B

Goat Anti-Mouse SDF-1 Alpha:Biotin

VEGF

Capture

AAM51

Rabbit Anti-Mouse VEGF

VEGF

Detection

AAM51B

Rabbit Anti-Mouse VEGF:Biotin

Rat

Table 3. Rat matched ELISA antibody pairs.

Specificity

Antibody

Catalog Number

Description

IgE

Capture

MCA191

Mouse Anti-Rat IgA Heavy Chain

IgG1

Capture

MCA190

Mouse Anti-Rat IgD

IgE

Capture

MCA193

Mouse Anti-Rat IgE

IgG1

Capture

MCA194

Mouse Anti-Rat IgG1 Heavy Chain

IgG2a

Capture

MCA278

Mouse Anti-Rat IgG2a Heavy Chain

IgG2b

Capture

MCA1294

Mouse Anti-Rat IgG2b Heavy Chain

IgG2c

Capture

MCA1295

Mouse Anti-Rat IgG2c Heavy Chain

IgM

Capture

MCA189

Mouse Anti-Rat IgM Heavy Chain

Kappa/Lambda Light Chain

Detection

MCA1296P

Mouse Anti-Rat Kappa/Lambda Light Chain:HRP

IL-1 beta

Capture

AAR15G

Rabbit Anti-Rat Interleukin-1 Beta

IL-1 beta

Detection

AAR15B

Rabbit Anti-Rat Interleukin-1 Beta:Biotin

RANTES

Capture

AAR18G

Rabbit Anti-Rat RANTES

RANTES

Detection

AAR18B

Rabbit Anti-Rat RANTES:Biotin

VEGF

Capture

AAR42

Rabbit Anti-Rat VEGF

VEGF

Detection

AAR42B

Rabbit Anti-Rat VEGF:Biotin

Bovine

Table 4. Bovine cytokine antibody pairs for ELISA.

Specificity & Format Format Clone
GM-CSF (capture) Purified Polyclonal
GM-CSF (detection) Biotin Polyclonal
IFN alpha A (capture) Purified Polyclonal
IFN alpha A (detection) Biotin Polyclonal
IFN beta (capture) Purified Polyclonal
IFN beta (detection) Biotin Polyclonal
IFN gamma (capture) Purified CC330
IFN gamma (detection) Biotin CC302
IL-1 alpha (capture) Purified Polyclonal
IL-1 alpha (detection) Biotin Polyclonal
IL-1F5 (capture) Purified Polyclonal
IL-1F5 (detection) Biotin Polyclonal
IL-1RA (capture) Purified Polyclonal
IL-1RA (detection) Biotin Polyclonal
IL-4 (capture) Purified CC313
IL-4 (detection) Biotin CC314
IL-5 (capture) Purified Polyclonal
IL-5 (detection) Biotin Polyclonal
IL-6 (capture) Purified Polyclonal
IL-6 (detection) Biotin Polyclonal
IL-8 (capture) Purified Polyclonal
IL-8 (detection) Biotin Polyclonal
IL-10 (capture) Purified CC318
IL-10 (detection) Biotin CC320
IL-13 (capture) Purified Polyclonal
IL-13 (detection) Biotin Polyclonal
IL-15 (capture) Purified Polyclonal
IL-15 (detection) Biotin Polyclonal
IL-17A (capture) Purified Polyclonal
IL-17A (detection) Biotin Polyclonal
IL-21 (capture) Purified Polyclonal
IL-21 (detection) Biotin Polyclonal
IL-22 (capture) Purified Monoclonal
IL-22 (detection) Purified Monoclonal
TNF-alpha (capture) Purified CC327
TNF-alpha (detection) Biotin CC328
TNF alpha (capture) Purified Polyclonal
TNF alpha (detection) Biotin Polyclonal