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Matched Antibody Pairs for ELISA
s
Overview
A matched antibody pair for ELISA consists of a capture antibody and a detection antibody. In a matched ELISA pair, each antibody is specific for a different and non-overlapping region or epitope of the antigen. By design, matched pairs function in a sandwich ELISA set-up; for further information on sandwich ELISA, guides and protocols go to the main ELISA page. Figure 1 below shows the sandwich ELISA protocol.
Fig. 1. Typical sandwich ELISA set-up using matched antibody pairs.
We have validated our matched antibody pairs in sandwich ELISA tests, removing any concerns about performance and reagent selection; the assay data can be found on the respective product pages. We also offer protein standards, as controls and for generation of a standard curve, for a subset of the matched ELISA pairs.
View our recommended ELISA protocol for use with matched antibody pairs.
See the tables below for our range of matched ELISA antibody pairs for the following species:
Human
Table 1. Human matched ELISA antibody pairs.
Specificity |
Antibody Type |
Catalog Number |
Description |
|---|---|---|---|
|
A2M |
Capture |
Mouse Anti-Human A2M |
|
|
A2M |
Detection |
Mouse Anti-Human A2M:Biotin |
|
|
Adiponectin |
Capture |
Mouse Anti-Human Adiponectin |
|
|
Adiponectin |
Detection |
Mouse Anti-Human Adiponectin:Biotin |
|
|
BDNF |
Capture |
Rabbit Anti-Human BDNF |
|
|
BDNF |
Detection |
Rabbit Anti-Human BDNF:Biotin |
|
|
BMP-2 |
Capture |
Rabbit Anti-Human BMP-2 |
|
|
BMP-2 |
Detection |
Rabbit Anti-Human BMP-2:Biotin |
|
|
CCL2 |
Capture |
Mouse Anti-Human CCL2 |
|
|
CCL2 |
Detection |
Mouse Anti-Human CCL2:Biotin |
|
|
CCL3 |
Capture |
Mouse Anti-Human CCL3 |
|
|
CCL3 |
Detection |
Mouse Anti-Human CCL3:Biotin |
|
|
CCL3L1 |
Capture |
Goat Anti-Human CCL3L1 |
|
|
CCL3L1 |
Detection |
Goat Anti-Human CCL3L1:Biotin |
|
|
CCL8 |
Capture |
Mouse Anti-Human CCL8 |
|
|
CCL8 |
Detection |
Mouse Anti-Human CCL8:Biotin |
|
|
CCL13 |
Capture |
Mouse Anti-Human CCL13 |
|
|
CCL13 |
Detection |
Mouse Anti-Human CCL13:Biotin |
|
|
CCL14 |
Capture |
Mouse Anti-Human CCL14 |
|
|
CCL14 |
Detection |
Mouse Anti-Human CCL14:Biotin |
|
|
CCL17 |
Capture |
Rabbit Anti-Human CCL17 |
|
|
CCL17 |
Detection |
Rabbit Anti-Human CCL17:Biotin |
|
|
CCL17 |
Capture |
Mouse Anti-Human CCL17 |
|
|
CCL17 |
Detection |
Mouse Anti-Human CCL17:Biotin |
|
|
CCL20 |
Capture |
Mouse Anti-Human CCL20 |
|
|
CCL20 |
Detection |
Mouse Anti-Human CCL20:Biotin |
|
|
CCL24 |
Capture |
Mouse Anti-Human CCL24 |
|
|
CCL24 |
Detection |
Mouse Anti-Human CCL24:Biotin |
|
|
CCL27 |
Capture |
Mouse Anti-Human CCL27 |
|
|
CCL27 |
Detection |
Mouse Anti-Human CCL27:Biotin |
|
|
CD30 |
Capture |
Mouse Anti-Human CD30 |
|
|
CD30 |
Detection |
Mouse Anti-Human CD30:Biotin |
|
|
CD62P |
Capture |
Mouse Anti-Human CD62P |
|
|
CD62P |
Detection |
Mouse Anti-Human CD62P:Biotin |
|
|
CD95 |
Capture |
Mouse Anti-Human CD95 |
|
|
CD95 |
Detection |
Mouse Anti-Human CD95:Biotin |
|
|
CD120b |
Capture |
Rabbit Anti-Human CD120b |
|
|
CD120b |
Detection |
Rabbit Anti-Human CD120b:Biotin |
|
|
CD130 |
Capture |
Mouse Anti-Human CD130 |
|
|
CD130 |
Detection |
Mouse Anti-Human CD130:Biotin |
|
|
CD258 |
Capture |
Mouse Anti-Human CD258 |
|
|
CD258 |
Detection |
Mouse Anti-Human CD258:Biotin |
|
|
CRP |
Capture |
Mouse Anti-Human CRP |
|
|
CRP |
Detection |
Mouse Anti-Human CRP:Biotin |
|
|
CTGF |
Capture |
Rabbit Anti-Human CTGF |
|
|
CTGF |
Detection |
Rabbit Anti-Human CTGF:Biotin |
|
|
CXCL7 |
Capture |
Mouse Anti-Human CXCL7 |
|
|
CXCL7 |
Detection |
Mouse Anti-Human CXCL7:Biotin |
|
|
CXCL9 |
Capture |
Mouse Anti-Human CXCL9 |
|
|
CXCL9 |
Detection |
Mouse Anti-Human CXCL9:Biotin |
|
|
CXCL10 |
Capture |
Mouse Anti-Human CXCL10 |
|
|
CXCL10 |
Detection |
Mouse Anti-Human CXCL10:Biotin |
|
|
CXCL13 |
Capture |
Rabbit Anti-Human CXCL13 |
|
|
CXCL13 |
Detection |
Rabbit Anti-Human CXCL13:Biotin |
|
|
CXCL16 |
Capture |
Mouse Anti-Human CXCL16 |
|
|
CXCL16 |
Detection |
Mouse Anti-Human CXCL16:Biotin |
|
|
Cystatin C |
Capture |
Mouse Anti-Human Cystatin C |
|
|
Cystatin C |
Detection |
Mouse Anti-Human Cystatin C:Biotin |
|
|
Defensin beta 2 |
Capture |
Goat Anti-Human Defensin Beta 2 |
|
|
Defensin beta 2 |
Detection |
Goat Anti-Human Defensin Beta 2:Biotin |
|
|
EGF |
Capture |
Rabbit Anti-Human EGF |
|
|
EGF |
Detection |
Rabbit Anti-Human EGF:Biotin |
|
|
FGF basic |
Capture |
Rabbit Anti-Human FGF Basic |
|
|
FGF basic |
Detection |
Rabbit Anti-Human FGF Basic:Biotin |
|
|
Factor H |
Capture |
Mouse Anti-Human Complement Factor H |
|
|
Factor H |
Detection |
Mouse Anti-Human Complement Factor H:Biotin |
|
|
Fibrinogen |
Capture |
Mouse Anti-Human Fibrinogen |
|
|
Fibrinogen |
Detection |
Mouse Anti-Human Fibrinogen:Biotin |
|
|
G-CSF |
Capture |
Rat Anti-Human G-CSF |
|
|
G-CSF |
Detection |
Rat Anti-Human G-CSF:Biotin |
|
|
G-CSF |
Capture |
Rabbit Anti-Human G-CSF |
|
|
G-CSF |
Detection |
Rabbit Anti-Human G-CSF:Biotin |
|
|
Granzyme B |
Capture |
Mouse Anti-Granzyme B |
|
|
Granzyme B |
Detection |
Mouse Anti-Granzyme B:Biotin |
|
|
GM-CSF |
Capture |
Mouse Anti-Human GM-CSF |
|
|
GM-CSF |
Detection |
Mouse Anti-Human GM-CSF:Biotin |
|
|
Haptoglobin |
Capture |
Mouse Anti-Human Haptoglobin |
|
|
Haptoglobin |
Detection |
Mouse Anti-Human Haptoglobin:Biotin |
|
|
IFN Beta |
Capture |
Mouse Anti-Human IFN Beta |
|
|
IFN Beta |
Detection |
Mouse Anti-Human IFN Beta:Biotin |
|
|
IFN gamma |
Capture |
Mouse Anti-Human IFN Gamma |
|
|
IFN gamma |
Detection |
Mouse Anti-Human IFN Gamma:Biotin |
|
|
IFN gamma |
Capture |
Rabbit Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Rabbit Anti-Human Interferon Gamma:Biotin |
|
|
IFN gamma |
Capture |
Human Anti-Human Interferon Gamma |
|
|
IFN gamma |
Detection |
Human Anti-Human Interferon Gamma |
|
|
IGF-I |
Capture |
Rabbit Anti-Human Insulin-Like Growth Factor I |
|
|
IGF-I |
Detection |
Rabbit Anti-Human Insulin-Like Growth Factor I:Biotin |
|
|
IL-1RA |
Capture |
Rabbit Anti-Human Interleukin-1RA |
|
|
IL-1RA |
Detection |
Rabbit Anti-Human Interleukin-1RA:Biotin |
|
|
IL-2 |
Capture |
Rabbit Anti-Human Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Human Interleukin-2:Biotin |
|
|
IL-2 |
Capture |
Rat anti Human Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Human Interleukin-2:Biotin |
|
|
IL-4 |
Capture |
Mouse Anti-Human Interleukin-4 |
|
|
IL-4 |
Detection |
Mouse Anti-Human Interleukin-4:Biotin |
|
|
IL-5 |
Capture |
Rabbit Anti-Human Interleukin-5 |
|
|
IL-5 |
Detection |
Rabbit Anti-Human Interleukin-5:Biotin |
|
|
IL-6 |
Capture |
Mouse Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Mouse Anti-Human Interleukin-6:Biotin |
|
|
IL-6 |
Capture |
Rat Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Rat Anti-Human Interleukin-6:Biotin |
|
|
IL-6 |
Capture |
Rabbit Anti-Human Interleukin-6 |
|
|
IL-6 |
Detection |
Rabbit Anti-Human Interleukin-6:Biotin |
|
|
IL-8 |
Capture |
Mouse Anti-Human Interleukin-8 |
|
|
IL-8 |
Detection |
Mouse Anti-Human Interleukin-8:Biotin |
|
|
IL-11 |
Capture |
Mouse Anti-Human Interleukin-11 |
|
|
IL-11 |
Detection |
Mouse Anti-Human Interleukin-11:Biotin |
|
|
IL-12 p40 |
Capture |
Mouse Anti-Human Interleukin-12p40 |
|
|
IL-12 p40 |
Detection |
Mouse Anti-Human Interleukin-12p40:Biotin |
|
|
IL-17A |
Capture |
Mouse Anti-Human Interleukin-17A |
|
|
IL-17A |
Detection |
Mouse Anti-Human Interleukin-17A:Biotin |
|
|
IL-17A/F |
Capture |
Mouse Anti-Human Interleukin-17A/F |
|
|
IL-17A/F |
Detection |
Mouse Anti-Human Interleukin-17A/F:Biotin |
|
|
IL-17F |
Capture |
Mouse Anti-Human Interleukin-17F |
|
|
IL-17F |
Detection |
Mouse Anti-Human Interleukin-17F:Biotin |
|
|
IL-18BP |
Capture |
Mouse Anti-Human Interleukin-18BP |
|
|
IL-18BP |
Detection |
Mouse Anti-Human Interleukin-18BP:Biotin |
|
|
IL-25 |
Capture |
Mouse Anti-Human Interleukin-25 |
|
|
IL-25 |
Detection |
Mouse Anti-Human Interleukin-25:Biotin |
|
|
IL-28a |
Capture |
Mouse Anti-Human Interleukin-28a |
|
|
IL-28a |
Detection |
Mouse Anti-Human Interleukin-28a:Biotin |
|
|
IL-31 |
Capture |
Mouse Anti-Human Interleukin-31 |
|
|
IL-31 |
Detection |
Mouse Anti-Human Interleukin-31:Biotin |
|
|
IL-32 |
Capture |
Mouse Anti-Human Interleukin-32 |
|
|
IL-32 |
Detection |
Mouse Anti-Human Interleukin-32:Biotin |
|
|
IL-34 |
Capture |
Mouse Anti-Human Interleukin-34 |
|
|
IL-34 |
Detection |
Mouse Anti-Human Interleukin-34:Biotin |
|
|
LOX-1 |
Capture |
Mouse Anti-Human LOX-1 |
|
|
LOX-1 |
Detection |
Mouse Anti-Human LOX-1:Biotin |
|
|
MMP-1 |
Capture |
Mouse Anti-Human MMP-1 |
|
|
MMP-1 |
Detection |
Mouse Anti-Human MMP-1:Biotin |
|
|
MMP-2 |
Capture |
Mouse Anti-Human MMP-2 |
|
|
MMP-2 |
Detection |
Mouse Anti-Human MMP-2:Biotin |
|
|
MMP-3 |
Capture |
Mouse Anti-Human MMP-3 |
|
|
MMP-3 |
Detection |
Mouse Anti-Human MMP-3:Biotin |
|
|
MMP-9 |
Capture |
Mouse Anti-Human MMP-9 |
|
|
MMP-9 |
Detection |
Mouse Anti-Human MMP-9:Biotin |
|
|
MPO |
Capture |
Mouse Anti-Human Myeloperoxidase |
|
|
MPO |
Detection |
Mouse Anti-Human Myeloperoxidase:Biotin |
|
|
NGAL |
Capture |
Mouse Anti-Human NGAL |
|
|
NGAL |
Detection |
Mouse Anti-Human NGAL:Biotin |
|
|
Osteopontin |
Capture |
Mouse Anti-Human Osteopontin |
|
|
Osteopontin |
Detection |
Mouse Anti-Human Osteopontin:Biotin |
|
|
PDGF-BB |
Capture |
Mouse Anti-Human PDGF-BB |
|
|
PDGF-BB |
Detection |
Mouse Anti-Human PDGF-BB:Biotin |
|
|
Prolactin |
Capture |
Mouse Anti-Human Prolactin |
|
|
Prolactin |
Detection |
Mouse Anti-Human Prolactin:Biotin |
|
|
Prolactin |
Capture |
Mouse Anti-Human Prolactin |
|
|
Prolactin |
Detection |
Mouse Anti-Human Prolactin |
|
|
RAGE |
Capture |
Mouse Anti-Human RAGE |
|
|
RAGE |
Detection |
Mouse Anti-Human RAGE:Biotin |
|
|
RANTES |
Capture |
Rabbit Anti-Human RANTES |
|
|
RANTES |
Detection |
Rabbit Anti-Human RANTES:Biotin |
|
|
SAP |
Capture |
Mouse Anti-Human SAP |
|
|
SAP |
Detection |
Mouse Anti-Human SAP:Biotin |
|
|
SDF-1 alpha |
Capture |
Rabbit Anti-Human SDF-1 Alpha |
|
|
SDF-1 alpha |
Detection |
Rabbit Anti-Human SDF-1 Alpha:Biotin |
|
|
TIMP-1 |
Capture |
Mouse Anti-Human TIMP-1 |
|
|
TIMP-1 |
Detection |
Mouse Anti-Human TIMP-1:Biotin |
|
|
TNF alpha |
Capture |
Goat Anti-Human TNF Alpha |
|
|
TNF alpha |
Detection |
Goat Anti-Human TNF Alpha:Biotin |
|
|
tPA |
Capture |
Mouse Anti-Human tPA |
|
|
tPA |
Detection |
Mouse Anti-Human tPA:Biotin |
|
|
TSLP |
Capture |
Mouse Anti-Human TSLP |
|
|
TSLP |
Detection |
Mouse Anti-Human TSLP:Biotin |
|
|
VEGF |
Capture |
Mouse Anti-Human VEGF |
|
|
VEGF |
Detection |
Mouse Anti-Human VEGF:Biotin |
|
|
VEGF |
Capture |
Goat Anti-Human VEGF |
|
|
VEGF |
Detection |
Goat Anti-Human VEGF:Biotin |
|
|
vWF |
Capture |
Mouse Anti-Human vWF |
|
|
vWF |
Detection |
Mouse Anti-Human vWF:Biotin |
|
|
WNT-3a |
Capture |
Rabbit Anti-Human WNT-3a |
|
|
WNT-3a |
Detection |
Rabbit Anti-Human WNT-3a:Biotin |
|
|
YKL-40 |
Capture |
Mouse Anti-Human YKL-40 |
|
|
YKL-40 |
Detection |
Mouse Anti-Human YKL-40:Biotin |
Mouse
Table 2. Mouse matched ELISA antibody pairs.
Specificity |
Antibody Type |
Catalog Number |
Description |
|---|---|---|---|
|
CXCL1 |
Capture |
Rabbit Anti-Mouse CXCL1 |
|
|
CXCL1 |
Detection |
Rabbit Anti-Mouse CXCL1:Biotin |
|
|
IgG1 |
Capture |
Rat Anti-Mouse IgG1 |
|
|
IgG1 |
Detection |
Rat Anti-Mouse IgG1 Heavy Chain:HRP |
|
|
IgG2a |
Capture |
Rat Anti-Mouse IgG2a Heavy Chain |
|
|
IgG2a |
Detection |
Rat Anti-Mouse IgG2a Heavy Chain:HRP |
|
|
IgG2b |
Capture |
Rat Anti-Mouse IgG2b |
|
|
IgG2b |
Detection |
Rat Anti-Mouse IgG2b:HRP |
|
|
IgG3 |
Capture |
Rat Anti-Mouse IgG3 |
|
|
IgG3 |
Detection |
Rat Anti-Mouse IgG3 Heavy Chain:Biotin |
|
|
IgM |
Capture |
Rat Anti-Mouse IgM Heavy Chain |
|
|
IgM |
Detection |
Rat Anti-Mouse IgM Heavy Chain:Biotin |
|
|
IL-1 beta |
Capture |
Rabbit Anti-Mouse Interleukin-1 Beta |
|
|
IL-1 beta |
Detection |
Rabbit Anti-Mouse Interleukin-1 Beta:Biotin |
|
|
IL-2 |
Capture |
Rabbit Anti-Mouse Interleukin-2 |
|
|
IL-2 |
Detection |
Rabbit Anti-Mouse Interleukin-2:Biotin |
|
|
IL-13 |
Capture |
Rabbit Anti-Mouse Interleukin-13 |
|
|
IL-13 |
Detection |
Rabbit Anti-Mouse Interleukin-13:Biotin |
|
|
IL-21 |
Capture |
Rabbit Anti-Mouse Interleukin-21 |
|
|
IL-21 |
Detection |
Rabbit Anti-Mouse Interleukin-21:Biotin |
|
|
IP-10 |
Capture |
Rabbit Anti-Mouse IP-10 |
|
|
IP-10 |
Detection |
Rabbit Anti-Mouse IP-10:Biotin |
|
|
MIP-2 |
Capture |
Rabbit Anti-Mouse MIP-2 |
|
|
MIP-2 |
Detection |
Rabbit Anti-Mouse MIP-2:Biotin |
|
|
SDF-1 alpha |
Capture |
Goat Anti-Mouse SDF-1 Alpha |
|
|
SDF-1 alpha |
Detection |
Goat Anti-Mouse SDF-1 Alpha:Biotin |
|
|
VEGF |
Capture |
Rabbit Anti-Mouse VEGF |
|
|
VEGF |
Detection |
Rabbit Anti-Mouse VEGF:Biotin |
Rat
Table 3. Rat matched ELISA antibody pairs.
Specificity |
Antibody |
Catalog Number |
Description |
|---|---|---|---|
|
IgE |
Capture |
Mouse Anti-Rat IgA Heavy Chain |
|
|
IgG1 |
Capture |
Mouse Anti-Rat IgD |
|
|
IgE |
Capture |
Mouse Anti-Rat IgE |
|
|
IgG1 |
Capture |
Mouse Anti-Rat IgG1 Heavy Chain |
|
|
IgG2a |
Capture |
Mouse Anti-Rat IgG2a Heavy Chain |
|
|
IgG2b |
Capture |
Mouse Anti-Rat IgG2b Heavy Chain |
|
|
IgG2c |
Capture |
Mouse Anti-Rat IgG2c Heavy Chain |
|
|
IgM |
Capture |
Mouse Anti-Rat IgM Heavy Chain |
|
|
Kappa/Lambda Light Chain |
Detection |
Mouse Anti-Rat Kappa/Lambda Light Chain:HRP |
|
|
IL-1 beta |
Capture |
Rabbit Anti-Rat Interleukin-1 Beta |
|
|
IL-1 beta |
Detection |
Rabbit Anti-Rat Interleukin-1 Beta:Biotin |
|
|
RANTES |
Capture |
Rabbit Anti-Rat RANTES |
|
|
RANTES |
Detection |
Rabbit Anti-Rat RANTES:Biotin |
|
|
VEGF |
Capture |
Rabbit Anti-Rat VEGF |
|
|
VEGF |
Detection |
Rabbit Anti-Rat VEGF:Biotin |
Bovine
Table 4. Bovine cytokine antibody pairs for ELISA.
| Specificity & Format | Format | Clone |
|---|---|---|
| GM-CSF (capture) | Purified | Polyclonal |
| GM-CSF (detection) | Biotin | Polyclonal |
| IFN alpha A (capture) | Purified | Polyclonal |
| IFN alpha A (detection) | Biotin | Polyclonal |
| IFN gamma (capture) | Purified | CC330 |
| IFN gamma (detection) | Biotin | CC302 |
| IL-1RA (capture) | Purified | Polyclonal |
| IL-1RA (detection) | Biotin | Polyclonal |
| IL-4 (capture) | Purified | CC313 |
| IL-4 (detection) | Biotin | CC314 |
| IL-5 (capture) | Purified | Polyclonal |
| IL-5 (detection) | Biotin | Polyclonal |
| IL-6 (capture) | Purified | Polyclonal |
| IL-6 (detection) | Biotin | Polyclonal |
| IL-8 (capture) | Purified | Polyclonal |
| IL-8 (detection) | Biotin | Polyclonal |
| IL-10 (capture) | Purified | CC318 |
| IL-10 (detection) | Biotin | CC320 |
| IL-17A (capture) | Purified | Polyclonal |
| IL-17A (detection) | Biotin | Polyclonal |
| IL-22 (capture) | Purified | Monoclonal |
| IL-22 (detection) | Purified | Monoclonal |
| TNF-alpha (capture) | Purified | CC327 |
| TNF-alpha (detection) | Biotin | CC328 |
| TNF alpha (capture) | Purified | Polyclonal |
| TNF alpha (detection) | Biotin | Polyclonal |