VEGF antibody
Goat anti Human VEGF antibody (AHP831) used for the identification of VEGF expressing cells in atherosclerotic plaques by immunohistochemistry.
Image caption:
Example of immunostaining of different antibodies.
A: Overview. C: CD68 positive macrophages. E: VEGFR2. F: VEGF. H: Dll4. Black bars represent 1000 μm (A) and 200 μm (B-H).
From: Hjelmgren O, Gellerman K, Kjelldahl J, Lindahl P, Bergström GML (2016)
Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS ONE 11(12): e0166918.
Goat anti Human VEGF antibody recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.
The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).
The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage, the addition of 0.09% soidum azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human VEGF
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of reconstitution
- Guarantee
- 12 months from date of reconstitution.
More Information
Applications of VEGF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.25 | 1.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml | |
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 0.05 | 0.10ug/ml | |
| Immunohistology - Paraffin 1 | 5.0 | 15.0ug/ml | |
| Western Blotting | 0.1 | 0.2ug/ml |
- 1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Citrate buffer, pH6.0, is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This biotinylated human VEGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human VEGF antibody (AHP831) as the capture reagent and recombinant human VEGF (PHP091) as the standard.
- ELISA
- This purified human VEGFantibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human VEGF antibody (AHP831B) as the detection reagent and recombinant human VEGF (PHP091) as the standard.
- Histology Positive Control Tissue
- Human breast invasive ductal carcinoma
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF (PHP091) as the positive control.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF (PHP091) as the positive control.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human VEGF | AHP831 | E FN P* WB | 0.1 mg |
|
|
| Goat anti Human VEGF:Biotin | AHP831B | E WB | 50 µg |
|
|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Positive Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Human VEGF | PHP091 | E FN WB | 10 µg |
|
|
| Recombinant Human VEGF | PHP091 | E FN WB | 10 µg |
|
Product Specific References
References for VEGF antibody
-
Hjelmgren, O. et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS One. 11 (12): e0166918. -
Gamliel, M. et al. (2018) Trained Memory of Human Uterine NK Cells Enhances Their Function in Subsequent Pregnancies.
Immunity. 48 (5): 951-962.e5.
Further Reading
-
Neufeld, G. et al. (1999) Vascular endothelial growth factor (VEGF) and its receptors.
FASEB J. 13: 9-22.
