VEGF antibody

Goat anti Human VEGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
VEGF

Product Code Applications Pack Size List Price Your Price Qty
AHP831
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E FN P * WB 0.1 mg loader
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Goat anti Human VEGF antibody recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.

The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage, the addition of 0.09% soidum azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 0.05 0.10ug/ml
Immunohistology - Paraffin 1 5.0 15.0ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Citrate buffer, pH6.0, is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This purified human VEGFantibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human VEGF antibody (AHP831B) as the detection reagent and recombinant human VEGF (PHP091) as the standard.
Histology Positive Control Tissue
Human breast invasive ductal carcinoma
Western Blotting
This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF (PHP091) as the positive control.

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Description Rabbit anti Goat IgG (Fc):HRP
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Description Rabbit anti Goat IgG (Fc):FITC

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Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0
Goat anti Human VEGF:Biotin AHP831B E WB 50 µg loader
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Description Goat anti Human VEGF:Biotin

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human VEGF PHP091 E FN WB 10 µg loader
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Description Recombinant Human VEGF

References for VEGF antibody

  1. Hjelmgren, O. et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
    PLoS One. 11 (12): e0166918.
  2. Gamliel, M. et al. (2018) Trained Memory of Human Uterine NK Cells Enhances Their Function in Subsequent Pregnancies.
    Immunity. 48 (5): 951-962.e5.

Further Reading

  1. Neufeld, G. et al. (1999) Vascular endothelial growth factor (VEGF) and its receptors.
    FASEB J. 13: 9-22.

RRID
AB_2212845
UniProt
P15692
Entrez Gene
VEGFA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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