VEGF antibody
The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage, the addition of 0.09% soidum azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human VEGF
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of VEGF antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.25 | 1.0ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml | |
ELISA | 0.5 | 2.0ug/ml | |
Functional Assays | 0.05 | 0.10ug/ml | |
Immunohistology - Paraffin 1 | 5.0 | 15.0ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
- 1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Citrate buffer, pH6.0, is recommended for this purpose.
- ELISA
- This biotinylated human VEGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human VEGF antibody (AHP831) as the capture reagent and recombinant human VEGF (PHP091) as the standard.
- ELISA
- This purified human VEGFantibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human VEGF antibody (AHP831B) as the detection reagent and recombinant human VEGF (PHP091) as the standard.
- Histology Positive Control Tissue
- Human breast invasive ductal carcinoma
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF (PHP091) as the positive control.
- Western Blotting
- This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF (PHP091) as the positive control.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Human VEGF | AHP831 | E FN P* WB | 0.1 mg |
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Goat anti Human VEGF:Biotin | AHP831B | E WB | 50 µg |
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|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Positive Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Recombinant Human VEGF | PHP091 | E FN WB | 10 µg |
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Recombinant Human VEGF | PHP091 | E FN WB | 10 µg |
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Product Specific References
References for VEGF antibody
-
Hjelmgren, O. et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS One. 11 (12): e0166918. -
Gamliel, M. et al. (2018) Trained Memory of Human Uterine NK Cells Enhances Their Function in Subsequent Pregnancies.
Immunity. 48 (5): 951-962.e5.
Further Reading
-
Neufeld, G. et al. (1999) Vascular endothelial growth factor (VEGF) and its receptors.
FASEB J. 13: 9-22.
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