IgM antibody | LO-MM-3
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Rat anti Mouse IgM heavy chain recognizes murine IgM (mu heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.
Avidity = 2 x 108 M-1
Avidity = 2 x 108 M-1
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Polyclonal mouse IgM.
- Approx. Protein Concentrations
- Isotype concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01873
- P01872
- Entrez Gene
- Ighm
- Ighm
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0003823 antigen binding
- GO:0004888 transmembrane receptor activity
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0019882 antigen processing and presentation
- GO:0030890 positive regulation of B cell proliferation
- GO:0042571 immunoglobulin complex, circulating
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045022 early endosome to late endosome transport
- GO:0045807 positive regulation of endocytosis
- GO:0048471 perinuclear region of cytoplasm
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050853 B cell receptor signaling pathway
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 5ug/ml (coating) |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgM:Biotin | 302008 | C E IB | 1 mg |
|
|
| Goat anti Rat IgM:RPE | 302009 | F | 0.5 mg |
|
|
| Goat anti Rat IgM:HRP | STAR116P | E WB | 0.5 mg |
|
Product Specific References
References for IgM antibody
-
Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36. -
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71.
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