IgM antibody | LO-MM-3

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Rat anti Mouse IgM Heavy Chain

Product Type: Monoclonal Antibody
Clone: LO-MM-3
Isotype: IgM

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1293	E			1 mg

Rat anti Mouse IgM heavy chain recognizes murine IgM (mu heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.

Avidity = 2 x 10⁸ M^-1

Product Details

Target Species

Mouse

Product Form

Purified IgM - liquid

Preparation

Purified IgM prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1%	Sodium Azide

Immunogen

Polyclonal mouse IgM.

Approx. Protein Concentrations

Isotype concentration 1 mg/ml

Fusion Partners

Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01873 Related reagents; P01872 Related reagents
Entrez Gene: Ighm Related reagents; Ighm Related reagents
GO Terms: GO:0000187 activation of MAPK activity; GO:0005515 protein binding; GO:0002455 humoral immune response mediated by circulating immunoglobulin; GO:0003823 antigen binding; GO:0004888 transmembrane receptor activity; GO:0009897 external side of plasma membrane; GO:0019815 B cell receptor complex; GO:0019882 antigen processing and presentation; GO:0030890 positive regulation of B cell proliferation; GO:0042571 immunoglobulin complex, circulating; GO:0043410 positive regulation of MAPKKK cascade; GO:0045022 early endosome to late endosome transport; GO:0045807 positive regulation of endocytosis; GO:0048471 perinuclear region of cytoplasm; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050853 B cell receptor signaling pathway; GO:0016021 integral to membrane
Regulatory: For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			5ug/ml (coating)

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA: This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MM-9 (MCA199B) and Streptavidin:HRP (STAR5B) as the detection reagents.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Rat IgM:Biotin	302008	1 mg	C E IB
Goat anti Rat IgM:RPE	302009	0.5 mg	F
Goat anti Rat IgM:HRP	STAR116P	0.5 mg	E WB

Product Specific References

References for IgM antibody

Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36.
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71.

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