IgM antibody | LO-MM-3

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Rat anti Mouse IgM Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MM-3
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1293 E 1 mg
Rat anti Mouse IgM heavy chain recognizes murine IgM (mu heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.

Avidity = 2 x 108 M-1

Product Details

Target Species
Mouse
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Polyclonal mouse IgM.
Approx. Protein Concentrations
Isotype concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01873 Related reagents
P01872 Related reagents
Entrez Gene
Ighm Related reagents
Ighm Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml (coating)
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MM-9 (MCA199B) and Streptavidin:HRP (STAR5B) as the detection reagents.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgM:Biotin 302008 1 mg C E IB
Goat anti Rat IgM:RPE 302009 0.5 mg F
Goat anti Rat IgM:HRP STAR116P 0.5 mg E WB

Product Specific References

References for IgM antibody

  1. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  2. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.