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IgM antibody | LO-MM-3

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Anti Mouse IgM Heavy Chain Antibody, clone LO-MM-3 thumbnail image 1
Anti Mouse IgM Heavy Chain Antibody, clone LO-MM-3 gallery image 1
Published customer image:
Rat anti Mouse IgM antibody, clone LO-MM-3 (MCA1293) used to treat naïve B-cells in vitro prior to analysis of markers by flow cytometry.
Image caption:
Splenic naïve B cells from p38αfl/flCD19cre and p38αfl/fl mice were stimulated with IL-4 (5 ng/ml), anti-IgM (high concentration, 10 μg/ml), anti-IgM (low concentration, 1 μg/ml) + IL-4 (5 ng/ml), anti-IgM (low concentration, 1 μg/ml), anti-CD40 (5 μg/ml) for 3 days. MFI of activation markers (CD86, CD80 and CD69) was analyzed by flow cytometry (n=3 per group). Medium was used as a negative control. Data were representative of at least three independent experiments. Each symbol represents a representative sample. Small horizontal lines indicate the mean (± s.d.). Data were analyzed by two-tailed unpaired t-tests.

From: Wu J, Yang K, Cai S, Zhang X, Hu L, Lin F, Wu SQ, Xiao C, Liu WH, Han J.
A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation.
Nat Commun. 2022 Nov 28;13(1):7321.
doi: 10.1038/s41467-022-34969-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Rat anti Mouse IgM Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MM-3
Isotype
IgM
Specificity
IgM
Region
HEAVY CHAIN

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Product Code Applications Pack Size List Price Your Price Qty
MCA1293
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader

Rat anti Mouse IgM heavy chain recognizes murine IgM (mu heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.

Avidity = 2 x 108 M-1

Target Species
Mouse
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Polyclonal mouse IgM.
Approx. Protein Concentrations
Isotype concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml (coating)
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MM-9 (MCA199B) and Streptavidin:HRP (STAR5B) as the detection reagents.

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References for IgM antibody

  1. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  2. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.
  3. Wu, J. et al. (2022) A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation.
    Nat Commun. 13 (1): 7321.

RRID
AB_321868
UniProt
P01873
P01872
Entrez Gene
Ighm
Ighm
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane

MCA1293

160191 170203

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