IL-2 antibody | MQ1-17H12

100% Secure

Anti Human Interleukin-2 Antibody, clone MQ1-17H12 thumbnail image 1

Anti Human Interleukin-2 Antibody, clone MQ1-17H12 thumbnail image 2

Anti Human Interleukin-2 Antibody, clone MQ1-17H12 thumbnail image 3

Anti Human Interleukin-2 Antibody, clone MQ1-17H12 gallery image 1 — Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Rat anti Human IL-2 (**MCA1553F**). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated rat IgG2a (MCA6005F). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Mouse anti Human IL-2 (MCA1553F). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Anti Human Interleukin-2 Antibody, clone MQ1-17H12 gallery image 2 — Figure A. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Rat IgG2a (MCA6005PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Mouse anti Human IL-2 (**MCA1553PE**). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Anti Human Interleukin-2 Antibody, clone MQ1-17H12 gallery image 3 — Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 (**MCA1553**) as a capture reagent and biotinylated Rabbit anti Human IL-2 (AHP1032B) as a detection reagent with recombinant human IL-2 (PHP042) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected

Rat anti Human Interleukin-2:FITC

Rat anti Human Interleukin-2

Rat anti Human Interleukin-2:RPE

Product Type: Monoclonal Antibody
Clone: MQ1-17H12
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA1553F	F*	0.1 mg
MCA1553	E F*	0.5 mg
MCA1553PE	F*	100 Tests/1ml

Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 recognizes human interleukin-2 (IL-2), also known as T-cell growth factor or Aldesleukin. IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.

Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it ascts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkins et al. 1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotze et al. 1986). Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talal et al. 1983).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Cynomolgus monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1.0ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P60568 Related reagents
Entrez Gene: IL2 Related reagents
GO Terms: GO:0002903 negative regulation of B cell apoptosis; GO:0007155 cell adhesion; GO:0005125 cytokine activity; GO:0005134 interleukin-2 receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006955 immune response; GO:0007267 cell-cell signaling; GO:0008083 growth factor activity; GO:0019209 kinase activator activity; GO:0030101 natural killer cell activation; GO:0030217 T cell differentiation; GO:0030307 positive regulation of cell growth; GO:0030890 positive regulation of B cell proliferation; GO:0032740 positive regulation of interleukin-17 production; GO:0034105 positive regulation of tissue remodeling; GO:0042104 positive regulation of activated T cell proliferation; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0050729 positive regulation of inflammatory response
Regulatory: For research purposes only

Applications of IL-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹			Neat
ELISA
Flow Cytometry ¹
Flow Cytometry ¹			Neat

¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm^™ (Product Code BUF09) for this purpose.

¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm^™ (Product Code BUF09) for this purpose.

¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm^™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	1 ml	C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	0.5 mg	C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	1 mg	F
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	1 mg	C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	0.5 ml	F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)	STAR71D649GA	0.1 mg	F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	0.1 mg	F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	0.5 mg	C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	0.5 ml	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rat IgG2a Negative Control:FITC	MCA6005F	0.1 mg	F
Rat IgG2a Negative Control:RPE	MCA6005PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for IL-2 antibody

Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
BMC Cancer. 11: 410.
Moncrieffe, H. et al. (2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population.
J Immunol. 185 (1): 134-43.

View more products with IL-2 specificity