Convenient tools to detect any tagged protein
The use of epitope tags allows the detection of proteins where specific antibodies are not available. This eliminates the need to generate a new antibody for each protein saving time, resources and money.
We offer an extensive range of high performance great value epitope tag antibodies and anti-reporter protein antibodies. Our range includes V5 tag antibodies, His-6 tag antibodies, c-myc tag antibodies and DYKDDDDK tag monoclonal antibodies; for even better economy these popular antibodies are available in bulk.
An epitope tag is a biological structure or sequence, such as a protein or carbohydrate, which acts as an antigen that is recognized by an antibody. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. Epitope tags make it possible to detect proteins when no antibody is available. This technique can be used to characterize newly discovered proteins and low abundant proteins. Tags such as MBP and His are mainly used in protein purification while fluorescent tags, such as GFP are used for cell tracking and cellular localization experiments. Tags such as c-my, HA and DYKDDDDK on the other hand are mainly used in pull-down/immunoprecipitation experiments to verify and identify protein-protein interactions.
Key to Applications:
C=Snap Frozen - Acetone Fixed Immunohistological Sections, FC=Flow cytometry, IB=Immunoblotting, IF=immunofluorescence, IP=Immunoprecipitation, P=Formalin Fixed - Paraffin Embedded Immunohistological Sections, PP=Protein Purification, WB Western blotting
* Please refer to datasheet for further information.
Strep-tag® is a registered trademark of IBA, GmbH
BiaCore™ is a trademark of BiaCore Ab, a GE Healthcare Company.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.