Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Bio-Rad-Antibodies.com relies on third-party cookies to show you pricing, allow you to order online, and connect you to My Bio-Rad. Please amend your browser settings to enable third-party cookies and access this website’s full functionality.
Convenient tools to detect any tagged protein
The use of epitope tags allows the detection of proteins where specific antibodies are not available. This eliminates the need to generate a new antibody for each protein saving time, resources and money.
We offer an extensive range of high performance great value epitope tag antibodies and anti-reporter protein antibodies. Our range includes V5 tag antibodies, His-6 tag antibodies, c-myc tag antibodies and DYKDDDDK tag monoclonal antibodies; for even better economy these popular antibodies are available in bulk.
An epitope tag is a biological structure or sequence, such as a protein or carbohydrate, which acts as an antigen that is recognized by an antibody. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. Epitope tags make it possible to detect proteins when no antibody is available. This technique can be used to characterize newly discovered proteins and low abundant proteins. Tags such as MBP and His are mainly used in protein purification while fluorescent tags, such as GFP are used for cell tracking and cellular localization experiments. Tags such as c-my, HA and DYKDDDDK on the other hand are mainly used in pull-down/immunoprecipitation experiments to verify and identify protein-protein interactions.
Tag | Clone | Conjugates/Formats | Applications |
---|---|---|---|
BFP | Rabbit Polyclonal | Purified | WB |
CFP | Rabbit Polyclonal | Purified | WB |
mCherry | Clone 1, Rabbit Polyclonal | Purified | WB |
c-myc | 9E10, JAC6 | FC, ELISA, WB, IF, IP, C | |
DYKDDDDK | 6F7, rabbit polyclonal | Alexa Fluor® 647, FITC, HRP, RPE, Purified | FC, ELISA, WB, IF, IP,P |
GFP | Purified | ELISA, IF, WB, C, IB, P | |
GST | HRP, FITC, Purified | ELISA, WB, IF | |
HA | Rabbit polyclonal | Purified | ELISA, WB, P* |
His | AD1.1.10, ABD2.2.20, goat polyclonal | FC, ELISA, WB, IF, IP | |
MBP | Rabbit polyclonal, R29 | Serum, Purified | ELISA, IF, WB, IP |
RFP | Rabbit Polyclonal | Purified | WB |
S-tag | SBSTAGa, SBSTAGb, SBSTAGc | FITC, HRP, RPE, Purified | FC, PP, ELISA |
Strep | Strep-tag II, Strep-MAB | HRP, Purified | ELISA, WB |
VSV-G | Rabbit polyclonal IgG | Biotin, Purified | ELISA, WB, IF |
V5 | SV5-Pk1, SV5-Pk4, SV5-Pk5, SV5-Pk2 | FC, ELISA, C, WB, IF, IP, P, R |
Key to Applications:
C=Snap Frozen - Acetone Fixed Immunohistological Sections, FC=Flow cytometry, IB=Immunoblotting, IF=immunofluorescence, IP=Immunoprecipitation, P=Formalin Fixed - Paraffin Embedded Immunohistological Sections, PP=Protein Purification, WB Western blotting
* Please refer to datasheet for further information.
Strep-tag® is a registered trademark of IBA, GmbH
BiaCore™ is a trademark of BiaCore Ab, a GE Healthcare Company.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.