Epitope Tag Antibody References

Listed here are a selection of references and educational resources to help you to further characterize a selection of our tag antibodies. The references have been organized depending on the application used and can give you more information about detailed methods, uses and advantages of our products. Our V5 Tag antibody, clone SV5-Pk1 is highly cited - with 44 citations listed on Citeab.

c-myc Tag Antibody, Clone 9E10

Characterization

  1. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
    Proteins. 73:552-555.
  2. Hilpert, K. et al. (2001) Anti c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
    Protein Engineering 14:803-806.
  3. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
    Anticancer Res. 7:1299-1304.

Flow cytometry

  1. Borodina, I, et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.
    Microb Cell Fact 9:74-86.
  2. Koekemoer, A. L. et al. (2009) Myocyte stress 1 plays an important role in cellular hypertrophy and protection against apoptosis.
    FEBS Lett. 583:2964-2967.

Immunofluorescence

  1. Dubrana, K. et al. (2007) The processing of double-strand breaks and binding of single-strand-binding proteins RPA and Rad51 modulate the formation of ATR-kinase foci in yeast.
    J. Cell Science. 120:4209-4220.
  2. Qing, H. et al. (2004) Degradation of BACE by the ubiquitin-proteasome pathway.
    FASEB J. 18:1571-1574.
  3. Krugmann, S. et al. (2001) Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex.
    Current Biology 11:1645-1655.
  4. Martin, S.G., et al. (1999) Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast.
    Cell 97:621-633.

Immunohistology- paraffin

  1. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
    Anticancer Res. 7:1299-1304.

Immunoprecipitation

  1. Chalamalasetty, R.B. et al. (2006) Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission.
    J. Cell. Sci. 119:3008-3019.
  2. Qing, H. et al. (2004). Degradation of BACE by the ubiquitin-proteasome pathway.
    FASEB J. 18:1571-1574.
  3. Sharrock, R.A. et al. (2004) Heterodimerization of type II phytochromes in Arabidopsis
    PNAS 101:11500-11505.
  4. Krugmann, S. et al. (2001) Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex.
    Current Biology 11:1645-1655.
  5. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Mol. Cell. Biol. 5:3610-3616.

Western blotting

  1. Chalamalasetty, R.B. et al. (2006) Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission.
    J. Cell. Sci. 119:3008-3019.
  2. Sharrock, R.A. et al. (2004) Heterodimerization of type II phytochromes in Arabidopsis
    PNAS 101:11500-11505.
  3. Krugmann, S. et al. (2001) Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex.
    Current Biology 11:1645-1655.
  4. Light, Y. et al. (2001) 14-3-3 antagonizes Ras-mediated Raf-1 recruitment to the plasma membrane to maintain signaling fidelity.
    Mol. & Cell. Biol. 22:4984-4996.
  5. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Mol. Cell. Biol. 5:3610 -3616.

Histidine Tag Antibody, Clone AD1.1.10

ELISA

  1. Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
    PLoS One. 5:e10176.
  2. Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
    BMC Biotechnol.10:8.
  3. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
    Mol Immunol. 45:2570-2580.
  4. Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs
    J. Biol. Chem. 276:7346-7350.
  5. Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice.
    Immunol. 103:301-309.

Immunofluorescence

  1. Bahi, A. et al. (2008) The Role of Tissue-Type Plasminogen Activator System in Amphetamine-Induced Conditional Place Preference Extinction and Reinstatement.
    Neuropsychopharmacology 33:2726–2734.
  2. Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization.
    Genes Brain Behav. 7:244-256.
  3. Bahi, A. et al. (2005) In vivo gene silencing of CD81 by lentiviral expression of small interference RNAs suppresses cocaine-induced behaviour.
    J Neurochem. 92:1243-55.
  4. Bahi, A. et al. (2004) CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur.
    J. Neurosci. 19:1621-1633.

Immunohistochemistry

  1. Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization.
    Genes Brain Behav. 7:244-256.
  2. Bahi, A. et al. (2004) CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur.
    J. Neurosci. 19:1621-1633.

Immunoprecipitation

  1. Tang, R. & Rosen, S.D. (2009) Functional consequences of the subdomain organization of the sulfs.
    J. Biol. Chem. 284:21505-21514.
  2. Wrighton, K.H. et al. (2009) Transforming Growth Factor beta can stimulate Smad1 phosphorylation independently of Bone Morphogenic Protein receptors.
    J. Biol. Chem. 284:9755-9763.
  3. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
    J. Biol Chem. 282:22370-22375.

Western blotting

  1. Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
    BMC Biotechnol.10:8.
  2. Tang, R. & Rosen, S.D. (2009) Functional consequences of the subdomain organization of the sulfs.
    J. Biol. Chem. 284:21505-21514.
  3. Wrighton, K.H. et al. (2009) Transforming Growth Factor beta can stimulate Smad1 phosphorylation independently of Bone Morphogenic Protein receptors.
    J. Biol. Chem. 284:9755-9763.
  4. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
    Mol Immunol. 45:2570-2580.
  5. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
    J. Biol Chem. 282:22370-22375.
  6. Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
    BMC Immunol. 8:4
  7. Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs
    J. Biol. Chem. 276:7346-7350.

V5 (PK) tag antibody, clone SV5-Pk 

Characterization

  1. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
    J. Immunol. Methods. 224:141-150.
  2. Southern, J.A. et al. (1991) Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics.
    J. Gen. Virol. 72:1551-1557.

ChIP

  1. Ohtsuki, K. et al. (2010) Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters.
    Nucl. Acids. Res. 38:1805-1820.
  2. Schmidt, C.K. et al. (2009) Conserved features of cohesin binding along fission yeast chromosomes.
    Genome Biol. 10:R52.

Immunoblotting

  1. Sanchez Garcia, J. et al. (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the B-subunit.
    Nucleic Acids Research 32:3005-3016.

Immunofluorescence

  1. Duverger, O. et al. (2008) Molecular consequences of a frameshifted DLX3 mutant leading to Tricho-Dento-Osseous syndrome.
    J. Biol. Chem. 283:20198-20208.
  2. Ptushkina, M. et al. (2004) eIF4E isoform 2 in Schizosaccharomyces pombe is a novel stress-response factor.
    EMBO Rep. 5:311-316.
  3. Young, D.F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells
    J. Virol. 75:3363-3370.

Immunoprecipitation

  1. Boggio, R. et al. (2007) Targeting SUMO E1 to ubiquitin ligases: a viral strategy to counteract sumoylation.
    J. Biol. Chem. 282:15376-15382.
  2. Chew, E.H. et al. (2007) Substrate-mediated regulation of cullin neddylation.
    J. Biol. Chem. 282:17032-17040.
  3. Zhao, C. et al. (2005) Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
    Proc. Natl. Acad. Sci. U. S. A. 102:10200-10205.
  4. Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal
    EMBO J. 13:5410-5420.

Protein purification

  1. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding
    J.Virol. Methods 53:149-156.
  2. Randall, R.E. et al. (1994) Purification of antibody-antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming.
    Vaccine 12:351-358.
  3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrix-antibody antigen (SMAA) complexes as vaccines.
    Vaccine 11:1247-1252.

Western blotting

  1. O’Shea, J.M. & Perkins, N.D. (2010) Thr435 phosphorylation regulates RelA (p65) NF-kappaB subunit transactivation.
    Biochem. J. 426: 345–354.
  2. Duverger, O. et al. (2008) Molecular consequences of a frameshifted DLX3 mutant leading to Tricho-Dento-Osseous syndrome.
    J. Biol. Chem. 283: 20198-20208.
  3. Boggio, R. et al. (2007) Targeting SUMO E1 to ubiquitin ligases: a viral strategy to counteract sumoylation.
    J. Biol. Chem. 282: 15376-15382.
  4. Chew, E.H. et al. (2007) Substrate-mediated regulation of cullin neddylation.
    J. Biol. Chem. 282: 17032-17040.
  5. Zhao, C. et al. (2005) Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
    Proc. Natl. Acad. Sci. U. S. A. 102: 10200-10205.
  6. Jaffray, E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65
    Mol. Cell. Biol. 15: 2166-2172.
  7. Rodriguez, M.S. et al. (1995) Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein.
    Mol. Cell. Biol. 15: 2413-2419.
  8. Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal
    EMBO J. 13: 5410-5420.
  9. Hanke, T. et al. (1992) Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen.
    J. Gen. Virol. 73: 653-660.

 


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