Ideal tools for pull-down experiments
c-myc is a popular expression vector tag (EQKLISEEDL) often used in Western blotting and immunoprecipitation experiments. We offer two popular and highly cited c-myc tag antibody clones: 9E10 and JAC6.
Clone 9E10 is available in a variety of directly-conjugated formats including Alexa Fluor® 488 and 647 and DyLight® 549. Clone 9E10 detects the p62 c-myc proto-oncogene protein, which is involved in the regulation of cell cycle and cell growth processes. c-myc is primarily located in the cell nucleus, but is also known to be localised in the cytoplasm of certain cell lines(Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers.
For references describing the use of anti-c-myc tag antibodies in various applications, visit the epitope tag antibody references page.
c-myc tagged protein detected with Mouse anti c-myc:Alk Phos (MCA2200A)
Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody (MCA2200) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.