DYKDDDDK Tag antibody

Rabbit anti DYKDDDDK Tag

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
DYKDDDDK Tag

Product Code Applications Pack Size List Price Your Price Qty
AHP1074
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 50 µg loader
List Price Your Price
loader
AHP1074GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.

Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.

In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins.

Target Species
Synthetic Peptide
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Approx. Protein Concentrations
Pack Size: 50 µg
IgG concentration 0.5mg/ml
Pack Size: 0.1 mg
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/9000 1/450000
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for DYKDDDDK Tag antibody

  1. Keller, B. et al. (2016) Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT.
    J Exp Med. May 30. pii: jem.20151110. [Epub ahead of print]

Further Reading

  1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification.
    Bio/Technology 6: 1204-10.

RRID
AB_770097