DYKDDDDK Tag antibody

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Rabbit anti DYKDDDDK Tag:HRP

Rabbit anti DYKDDDDK Tag

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1074P E WB 0.1 mg
AHP1074 E WB 50 µg
AHP1074GA E WB 0.1 mg
Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.

Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.

In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody has been shown not to cross-react with any endogenous proteins.

Product Details

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Product Form
Purified IgG - liquid
Reconstitution
Reconstitute with 0.1ml deionised water. For long term storage at -20oC, add glycerol to a final concentration of 50%.
Antiserum Preparation
Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Gentamicin Sulfate
1% Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 50 µg
0.09% Sodium Azide (NaN3)
Pack Size: 0.1 mg
0.09%Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Approx. Protein Concentrations
IgG concentration 1mg/ml
Approx. Protein Concentrations
Pack Size: 50 µg
IgG concentration 0.5mg/ml
Pack Size: 0.1 mg
IgG concentration 0.5 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of reconstitution.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of DYKDDDDK Tag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/50,000
Western Blotting 1/1,000 1/5,000
ELISA 1/9000 1/450000
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for DYKDDDDK Tag antibody

  1. Keller, B. et al. (2016) Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT.
    J Exp Med. May 30. pii: jem.20151110. [Epub ahead of print]

Further Reading

  1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification.
    Bio/Technology 6: 1204-10.