DYKDDDDK Tag antibody
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Rabbit anti DYKDDDDK Tag
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- DYKDDDDK Tag
Antibody Quality and 
Unrivaled 
| Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins. |
- Target Species
- Synthetic Peptide
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
- Approx. Protein Concentrations
- Pack Size: 50 µgIgG concentration 0.5mg/mlPack Size: 0.1 mgIgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/9000 | 1/450000 |
| Western Blotting | |
1/2000 | 1/10000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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References for DYKDDDDK Tag antibody
-
Keller, B. et al. (2016) Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT.
J Exp Med. May 30. pii: jem.20151110. [Epub ahead of print]
Further Reading
-
Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification.
Bio/Technology 6: 1204-10.
- RRID
- AB_770097
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