GST antibody | AbD03937
Human anti GST antibody, clone AbD03937 detects glutathione-S-transferase (GST), a family of cytosolic dimeric isoenzymes that play an important role in detoxification by catalyzing the conjugation of reduced glutathione to a myriad of hydrophobic and electrophilic compounds. GSTs may also bind toxins and function as transport proteins.
Mammalian GSTs are ~45–55 kDa in size and are assigned to at least four generic classes: Alpha, Mu , Pi and Theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
GST is a commonly used tag in protein expression systems.
Mammalian GSTs are ~45–55 kDa in size and are assigned to at least four generic classes: Alpha, Mu , Pi and Theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
GST is a commonly used tag in protein expression systems.
Product Details
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant Glutathione-S-transferase.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of GST antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | 1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
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|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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| Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
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