c-Myc antibody | 9E10
Alexa-FluorA647 conjugated Mouse anti Human c-myc antibody, clone 9E10 (MCA2200A647) used for the evaluation of c-myc on dicidual NK cells demonstrating the correlation between c-myc and CD122 expression.
Image caption:
Expansion of specific iNKR subsets in decidual NK cells compared with matched pbNK. The expression of KIR2DL1, KIR2DL3/L2/S2, KIR3DL1, LILRB1, and NKG2A was examined by nine-color flow cytometry in matched pbNK and dNK from 21 donors. (A) Thirty-two distinct NK subsets were distinguished on the basis of the five iNKR in paired samples of pbNK and dNK (black and red graphs, respectively). The frequency of each subset from one representative donor is shown as a percentage of total NK cells. This provides a “fingerprint” of the iNKR repertoire, which is characteristic for each donor. The receptor combination for each subset is denoted by black-filled circles, with total number of iNKR in each subset indicated beneath. All NKG2A+ subsets are grouped to the right. The donor shown was genotyped as C1/C1. (B) The proportion of pbNK and dNK that express specific receptor combinations is shown as a percentage of total NK cells (mean ± SD, n = 21 matched pbNK and dNK pairs). The proportion of dNK that express two or more iNKR is significantly higher than in pbNK. (C) The percentage of dNK cells that are positive for Ki-67 was determined in each iNKR subset by intracellular staining. Control staining used isotype-matched mAb. The NK cells shown were gated on total KIR2DL1+ dNK as shown in Fig. 1A. (D) The percentages of dNK that are positive by intracellular staining for Ki-67 (•) and for CD122 (▴) are shown for all NKG2A+ dNK subsets (mean for each subset indicated by • or ▴ ± SD, derived from n = 11 donors). (E) Mean fluorescence intensity (MFI) of staining of each subset is shown following staining for CD122 and c-Myc in the NKG2A+ dNK subsets from (D). For each donor, MFI of each subset was expressed relative to the subset expressing NKG2A alone, which was set at 100%. Relative MFI of CD122 and c-Myc is highly correlated (Spearman rank correlation coefficient, R2 = 0.84, p <0.001).
From: Sharkey AM, Xiong S, Kennedy PR, Gardner L, Farrell LE, Chazara O, Ivarsson MA, Hiby SE, Colucci F, Moffett A.
Tissue-Specific Education of Decidual NK Cells.
J Immunol. 2015 Oct 1;195(7):3026-32.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti c-myc antibody, clone 9E10 (MCA2200) used for the detection of C-myc tagged Chs3versions derived from spheroblasts by western blotting
Image caption:
Topology determination of Chs3 using Proteinase K experiments with different myc-epitope tagged versions of Chs3. Cells expressing Chs313myc or different Chs33myc versions were converted into spheroplasts and treated with or without proteinase K in the presence or absence of Triton X-100. TCA precipitated proteins were solubilized and analyzed by sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) and Western blots using either: α-Chs3 or α-myc (A); and α-phosphofructokinase (PFK) (B) for immunostaining. Detection of PFK serves as a control for the integrity of the spheroplasts. Standard proteins are indicated with molecular masses given in kDa.
From: Gohlke S, Muthukrishnan S, Merzendorfer H.
In Vitro and In Vivo Studies on the Structural Organization of Chs3 from Saccharomyces cerevisiae.
Int J Mol Sci. 2017 Mar 25;18(4). pii: E702.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti c-Myc:Alexa Fluor® 488
Mouse anti c-Myc:Alexa Fluor® 647
Mouse anti c-Myc:Alk.Phos.
Mouse anti c-Myc:Biotin
Mouse anti c-Myc:FITC
Mouse anti c-Myc:HRP
Mouse anti c-Myc
Mouse anti c-Myc:RPE
Mouse anti c-Myc:DyLight®549
- Product Type
- Monoclonal Antibody
- Clone
- 9E10
- Isotype
- IgG1
Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines (Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999).
Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).
Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to DyLight®549 - liquid
- Reconstitution
- Reconstitute with 1.0ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- Pack Size: 20 µg, 0.1 mg, 1 mg0.09% Sodium Azide (NaN3)Pack Size: 2 mg
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of reconstitution
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01106 Related reagents
- Entrez Gene
- MYC Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001658 branching involved in ureteric bud morphogenesis
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0006879 cellular iron ion homeostasis
- GO:0007050 cell cycle arrest
- GO:0008283 cell proliferation
- GO:0042493 response to drug
- GO:0016563 transcription activator activity
- GO:0032204 regulation of telomere maintenance
- GO:0070888 E-box binding
- GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
- Regulatory
- For research purposes only
Applications of c-Myc antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat | ||
| Flow Cytometry 1 | Neat | ||
| ELISA | 1/500 | 1/5000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting 1 | 1/100 | 1/500 | |
| ELISA | 1/20 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting 1 | |||
| Flow Cytometry 1 | Neat | ||
| ELISA | 1/100 | 1/500 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting 1 | 1/100 | 1/500 | |
| ELISA | 1/100 | 1/500 | |
| Flow Cytometry 1 | Neat Pack Size: 20 µg, 0.1 mg, 1 mg 1/10 Pack Size: 2 mg |
1/10 Pack Size: 20 µg, 0.1 mg, 1 mg 1/50 Pack Size: 2 mg |
|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting 2 | 1/100 | 1/500 | |
| Western Blotting | Neat | 1/10 | |
| Flow Cytometry 1 | Neat | ||
| Immunofluorescence | Neat | 1/10 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 19E10 recognizes c-myc under non-reducing conditions
- 19E10 recognizes c-myc under non-reducing conditions
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 19E10 recognizes c-myc under non-reducing conditions
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2 9E10 recognizes c-myc under non-reducing conditions
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for c-Myc antibody
-
Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Mol Cell Biol. 5 (12): 3610-6. -
Spandidos, D.A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Anticancer Res. 7 (6): 1299-304. -
Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.
Microb Cell Fact. 9: 74. -
Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.
Biochem J. 404: 23-9. -
Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.
J Cell Biol. 187: 959-66. -
Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
Protein Eng. 14: 803-6. -
Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
Proteins. 73: 552-65. -
Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.
J Immunol. 184: 6359-66. -
Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.
J Biol Chem. 285: 26878-88. -
Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
Blood. 106: 2936-43. -
Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.
Mol Biol Cell. 21: 2996-3006. -
Rowshanravan, B. et al. (2014) RasGAP mediates neuronal survival in Drosophila through direct regulation of Rab5-dependent endocytosis.
J Cell Sci. 127: 2849-61. -
Taylor K et al. (2015) Nanocell targeting using engineered bispecific antibodies.
MAbs. 7 (1): 53-65. -
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159 (1-2): 29-40. -
Sharkey, A.M. et al. (2015) Tissue-Specific Education of Decidual NK Cells.
J Immunol. 195 (7): 3026-32. -
McGough, I.J. et al. (2014) Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling.
J Cell Sci. 127 (Pt 22): 4940-53. -
Gohlke, S. et al. (2017) In Vitro and In Vivo Studies on the Structural Organization of Chs3 from Saccharomyces cerevisiae.
Int J Mol Sci. 18 (4): pii: E702.
Further Reading
-
Nesbit, C. et al. (1999) MYC oncogenes and human neoplastic disease.
Oncogene. 18: 3004-16.
