c-Myc antibody | 9E10







Mouse anti c-Myc:Alexa Fluor® 488
Mouse anti c-Myc:Alexa Fluor® 647
Mouse anti c-Myc:Alk.Phos.
Mouse anti c-Myc:Biotin
Mouse anti c-Myc:FITC
Mouse anti c-Myc:HRP
Mouse anti c-Myc
Mouse anti c-Myc:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- 9E10
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2200A488 | F* | 100 Tests/1ml |
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MCA2200A647 | F* | 100 Tests/1ml |
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MCA2200A | C E P WB* | 0.1 mg |
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MCA2200B | C E P WB* | 0.1 mg |
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MCA2200F | F* | 0.1 mg |
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MCA2200P | C E P WB* | 0.1 mg |
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MCA2200T | C E F* P WB* | 20 µg |
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MCA2200G | C E F* P WB* | 2 mg |
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MCA2200GA | C E F* P WB* | 0.1 mg |
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MCA2200 | C E F* P WB* | 1 mg |
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MCA2200A700 | F* | 100 Tests/1ml |
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Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Epitope tag - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- Pack Size: 20 µg, 0.1 mg, 1 mg0.09% Sodium Azide (NaN3)Pack Size: 2 mg
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01106
- Entrez Gene
- MYC
- GO Terms
- GO:0005515 protein binding
- GO:0001658 branching involved in ureteric bud morphogenesis
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0006879 cellular iron ion homeostasis
- GO:0007050 cell cycle arrest
- GO:0008283 cell proliferation
- GO:0042493 response to drug
- GO:0016563 transcription activator activity
- GO:0032204 regulation of telomere maintenance
- GO:0070888 E-box binding
- GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of c-Myc antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | Neat | ||
Flow Cytometry 1 | Neat | ||
ELISA | 1/500 | 1/5000 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Western Blotting 1 | 1/100 | 1/500 | |
ELISA | 1/20 | 1/50 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Western Blotting 1 | |||
Flow Cytometry 1 | Neat | ||
ELISA | 1/100 | 1/500 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Western Blotting 1 | 1/100 | 1/500 | |
ELISA | 1/100 | 1/500 | |
Flow Cytometry 1 | Neat Pack Size: 20 µg, 0.1 mg, 1 mg 1/10 Pack Size: 2 mg |
1/10 Pack Size: 20 µg, 0.1 mg, 1 mg 1/50 Pack Size: 2 mg |
|
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Western Blotting 2 | 1/100 | 1/500 | |
Flow Cytometry 1 | Neat |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 19E10 recognizes c-myc under non-reducing conditions
- 19E10 recognizes c-myc under non-reducing conditions
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 19E10 recognizes c-myc under non-reducing conditions
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2 9E10 recognizes c-myc under non-reducing conditions
- 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:Alexa Fluor® 700 | MCA928A700 | F | 100 Tests/1ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for c-Myc antibody
-
Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Mol Cell Biol. 5 (12): 3610-6. -
Spandidos, D.A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Anticancer Res. 7 (6): 1299-304. -
Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.
Microb Cell Fact. 9: 74. -
Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.
Biochem J. 404: 23-9. -
Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.
J Cell Biol. 187: 959-66. -
Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
Protein Eng. 14: 803-6. -
Gohlke, S. et al. (2017) In Vitro and In Vivo Studies on the Structural Organization of Chs3 from Saccharomyces cerevisiae.
Int J Mol Sci. 18 (4): pii: E702. -
Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.
J Immunol. 184: 6359-66. -
Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.
J Biol Chem. 285: 26878-88. -
Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
Blood. 106: 2936-43. -
Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.
Mol Biol Cell. 21: 2996-3006. -
Rowshanravan, B. et al. (2014) RasGAP mediates neuronal survival in Drosophila through direct regulation of Rab5-dependent endocytosis.
J Cell Sci. 127: 2849-61. -
Taylor K et al. (2015) Nanocell targeting using engineered bispecific antibodies.
MAbs. 7 (1): 53-65. -
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159 (1-2): 29-40. -
Sharkey, A.M. et al. (2015) Tissue-Specific Education of Decidual NK Cells.
J Immunol. 195 (7): 3026-32. -
McGough, I.J. et al. (2014) Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling.
J Cell Sci. 127 (Pt 22): 4940-53. -
Frohnert, C. et al. (2014) Importin 7 and Nup358 promote nuclear import of the protein component of human telomerase.
PLoS One. 9 (2): e88887. -
Hage, N. et al. (2015) Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag.
Protein Expr Purif. 106: 25-30. -
Mann, J.K. & Park, S. (2015) Epitope-Specific Binder Design by Yeast Surface Display.
Methods Mol Biol. 1319: 143-54. -
Paraskevopoulou, V. et al. (2019) Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori.
Protein Expr Purif. 163: 105446. -
Lim, H.K. et al. (2010) Flow cytometric analysis of genetic FRET detectors containing variable substrate sequences.
Biotechnol Prog. 26 (6): 1765-71. -
Walker, L.M. et al. (2009) Efficient recovery of high-affinity antibodies from a single-chain Fab yeast display library.
J Mol Biol. 389 (2): 365-75. -
Matos, J. et al. (2013) Cell-cycle kinases coordinate the resolution of recombination intermediates with chromosome segregation.
Cell Rep. 4 (1): 76-86. -
Paraskevopoulou, V. et al. (2020) Structural and binding characterization of the LacdiNAc-specific adhesin (LabA; HopD) exodomain from Helicobacter pylori.
Curr Res Struct Biol. 15 Dec [Epub ahead of print]. -
Kalusche, S. et al. (2020) Lactobacilli Expressing Broadly Neutralizing Nanobodies against HIV-1 as Potential Vectors for HIV-1 Prophylaxis?
Vaccines (Basel). 8 (4) Dec 13 [Epub ahead of print]. -
Hollandsworth, H.M. et al. (2020) Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules.
Transl Oncol. 13 (12): 100857.
Further Reading
-
Nesbit, C. et al. (1999) MYC oncogenes and human neoplastic disease.
Oncogene. 18: 3004-16. -
Krauß, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
Proteins. 73: 552-65.
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