GST antibody | vpg66

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Mouse anti GST:Biotin

Mouse anti GST

Product Type
Monoclonal Antibody
Clone
vpg66
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1352B E WB 0.1 mg
MCA1352 IF WB 2 ml
Mouse anti GST antibody, clone vpg66 reacts with Glutathione-S-transferase

Product Details

Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 10mM Tris/HCl pH7.5 and 5-10% foetal calf serum
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
GST from Schistosoma japonicum.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from the date of despatch.

More Information

Regulatory
For research purposes only

Applications of GST antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/500
Immunofluorescence
Western Blotting 1/100 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

ELISA

Flow Cytometry

Immunofluorescence

Western Blotting

Product Specific References

References for GST antibody

  1. Harris, M.P. & Neil, J.C. (1994) Myristoylation-dependent binding of HIV-1 Nef to CD4.
    J Mol Biol. 241 (2): 136-42.
  2. Blüggel, M. et al. (2011) Toward protein biomarkers for allergy: CD4+ T cell proteomics in allergic and nonallergic subjects sampled in and out of pollen season.
    J Proteome Res. 10 (4): 1558-70.
  3. Shelton, H. & Harris, M. (2008) Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that contribute to the interaction.
    Virol J. 5: 24.
  4. Foster, T.L. et al. (2011) Cyclophilin A interacts with domain II of hepatitis C virus NS5A and stimulates RNA binding in an isomerase-dependent manner.
    J Virol. 85: 7460-4.
  5. Elfgang, C. et al. (1999) Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals.
    Proc Natl Acad Sci U S A. 96: 6229-34.
  6. Davis, M.P. (2008) Recombinant VP4 of human rhinovirus induces permeability in model membranes.
    J Virol. 82: 4169-74.
  7. Heger, P. et al. (1998) Multimer formation is not essential for nuclear export of human T-cell leukemia virus type 1 Rex trans-activator protein.
    J Virol. 72: 8659-68.
  8. Lischka, P. et al. (2001) A novel transferable nuclear export signal mediates CRM1-independent nucleocytoplasmic shuttling of the human cytomegalovirus transactivator protein pUL69.
    EMBO J. 20: 7271-83.