Histidine Tag antibody | ABD2.2.20

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Anti Penta Histidine Tag Antibody, clone ABD2.2.20 gallery image 1 — Detection of Human anti Human Vimentin (VMA00008) in a Western blot analysis of HEK293 whole cell lysate using Mouse anti Penta Histidine Tag:HRP (**MCA5995P**) at a 1/2000 dilution (lane 2). Arrow points to vimentin (molecular weight 54 kDa). Lane 4 shows secondary antibody only negative control.

Mouse anti Penta Histidine Tag:HRP

Product Type: Monoclonal Antibody
Clone: ABD2.2.20
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA5995P Datasheet	E WB SDS	125 µl	Log in		Get Quote

Summary
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Mouse anti penta histidine tag antibody, clone ABD2.2.20, recognizes proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will detect any accessible N-terminal, C-terminal or internal histidine tags and is highly recommended for use in assay development for the detection of his-tagged HuCAL primary antibodies.

Product Details

Target Species: Synthetic Peptide
Product Form: Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: Stabilized solution

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

Acknowledgements: His-tag is a registered trademark of EMD Biosciences.
Regulatory: For research purposes only

Applications of Histidine Tag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/1000	1/2000
Western Blotting		1/1000	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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