Histidine Tag antibody | ABD2.2.20
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WB ResetMouse anti Penta Histidine Tag:HRP
- Product Type
- Monoclonal Antibody
- Clone
- ABD2.2.20
- Isotype
- IgG1
- Specificity
- Histidine Tag
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| Mouse anti penta histidine tag antibody, clone ABD2.2.20, recognizes proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will detect any accessible N-terminal, C-terminal or internal histidine tags and is highly recommended for use in assay development for the detection of his-tagged HuCAL primary antibodies. |
- Target Species
- Synthetic Peptide
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Stabilized solution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- His-tag is a registered trademark of EMD Biosciences.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/1000 | 1/2000 |
| Western Blotting | |
1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
View more products with HISTIDINE specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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