Histidine Tag antibody | ABD2.2.20

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Mouse anti Penta Histidine Tag:HRP

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
125 µl loader

Mouse anti penta histidine tag antibody, clone ABD2.2.20, recognizes proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will detect any accessible N-terminal, C-terminal or internal histidine tags and is highly recommended for use in assay development for the detection of his-tagged HuCAL primary antibodies.

Product Details

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Stabilized solution

Storage Information

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

His-tag is a registered trademark of EMD Biosciences.
For research purposes only

Applications of Histidine Tag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/2000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Fluorescent Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra