Green Fluorescent Protein antibody

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Goat anti Green Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP975 E IF WB 0.1 mg
Goat anti green fluorescent protein antibody recognizes green fluorescent protein (GFP), a 27kD protein which is derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression. The antibody recognises wild type GFP, but will also detect recombinant and enhanced forms of GFP.

Product Details

Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to green fluorescent protein (GFP) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide
Immunogen
Fusion protein corresponding to full length GFP derived from Aequorea victoria
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P42212 Related reagents
GO Terms
GO:0006091 generation of precursor metabolites and energy
GO:0008218 bioluminescence
GO:0018298 protein-chromophore linkage
Regulatory
For research purposes only

Applications of Green Fluorescent Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/30,000
Immunofluorescence
Western Blotting 1/400 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Blue Fluorescent Protein AHP2985 50 µg WB
Rabbit anti Cyan Fluorescent Protein AHP2986 50 µg WB
Rabbit anti Red Fluorescent Protein AHP2987 50 µg WB

Application Based External Images

Western Blotting

Product Specific References

References for Green Fluorescent Protein antibody

  1. Wen, J, & Brogna, S. (2010) Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe.
    EMBO J. 29: 1537-51.
  2. Wuest, T. et al. (2011) The Herpes Simplex Virus-1 Transactivator Infected Cell Protein-4 Drives VEGF-A Dependent Neovascularization.
    PLoS Pathog. 7: e1002278.
  3. Al-Jubran, K. et al. (2013) Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.
    RNA. 19: 1669-83.
  4. Lampron, A. et al. (2013) Migration of bone marrow-derived cells into the central nervous system in models of neurodegeneration.
    J Comp Neurol. 521 (17): 3863-76.