Green Fluorescent Protein antibody

Goat anti Green Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Green Fluorescent Protein

Product Code Applications Pack Size List Price Your Price Qty
AHP975
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF WB 0.1 mg loader
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Search for Batch Specific Datasheets

Goat anti green fluorescent protein antibody recognizes green fluorescent protein (GFP), a 27kD protein which is derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression. The antibody recognises wild type GFP, but will also detect recombinant and enhanced forms of GFP.

Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to green fluorescent protein (GFP) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Fusion protein corresponding to full length GFP derived from Aequorea victoria
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/30,000
Immunofluorescence
Western Blotting 1/400 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Green Fluorescent Protein antibody

  1. Wen, J, & Brogna, S. (2010) Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe.
    EMBO J. 29: 1537-51.
  2. Wuest, T. et al. (2011) The Herpes Simplex Virus-1 Transactivator Infected Cell Protein-4 Drives VEGF-A Dependent Neovascularization.
    PLoS Pathog. 7: e1002278.
  3. Al-Jubran, K. et al. (2013) Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.
    RNA. 19: 1669-83.
  4. Lampron, A. et al. (2013) Migration of bone marrow-derived cells into the central nervous system in models of neurodegeneration.
    J Comp Neurol. 521 (17): 3863-76.
  5. Nicol, M.Q. et al. (2019) Lack of IFNγ signaling attenuates spread of influenza A virus in vivo and leads to reduced pathogenesis.
    Virology. 526: 155-164.

Immunofluorescence

Western Blotting

RRID
AB_566990
UniProt
P42212
GO Terms
GO:0006091 generation of precursor metabolites and energy
GO:0008218 bioluminescence
GO:0018298 protein-chromophore linkage
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"