V5-Tag antibody | SV5-Pk1

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Mouse anti V5-Tag:Alexa Fluor® 488

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:Alexa Fluor® 647

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:Alk. Phos.

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:Biotin

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:FITC

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:HRP

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:DyLight®405

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Mouse anti V5-Tag:DyLight®550

V5-Tag antibody, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5. It detects recombinant proteins, including transmembrane and secreted proteins tagged with this sequence.

Product Type
Monoclonal Antibody
Clone
SV5-Pk1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1360A488 F 100 Tests/1ml
MCA1360A647 F 1 ml
MCA1360A E WB 0.1 mg
MCA1360B C E WB 0.1 mg
MCA1360F IF 0.1 mg
MCA1360P E WB 0.1 mg
MCA1360GA C E F IF IP R WB 0.1 mg
MCA1360 C E F IF IP R WB 1 mg
MCA1360G C E F IF IP R WB 2 mg
MCA1360D405GA F IF 0.1 mg
MCA1360D550GA IF 0.1 mg
Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGL, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labelled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).

Product Details

Target Species
Viral
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®405 - liquid
Product Form
Purified IgG conjugated to DyLight®550 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Paramyxovirus Simian-Virus 5 (SV5)
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch

More Information

UniProt
P11207 Related reagents
GO Terms
GO:0003723 RNA binding
GO:0046872 metal ion binding
GO:0016070 RNA metabolic process
GO:0030683 evasion by virus of host immune response
Acknowledgements
This product is manufactured under an exclusive license from the University of St. Andrews, UK.

This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is manufactured under an exclusive license from the University of St. Andrews, UK.

This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is manufactured under an exclusive license from the University of St. Andrews, UK.
Acknowledgements
This product is manufactured under an exclusive license from the University of St. Andrews, UK.
Acknowledgements
This product is manufactured under an exclusive license from the University of St. Andrews, UK.
Acknowledgements
This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


This product is manufactured under an exclusive license from the University of St. Andrews, UK.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is manufactured under an exclusive license from the University of St. Andrews, UK.
Regulatory
For research purposes only

Applications of V5-Tag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
ELISA
Western Blotting 1/1000
ELISA 1/250 1/2500
Immunohistology - Frozen
Western Blotting 1/1000 1/5000
Immunofluorescence 1/100
ELISA
Western Blotting 1/1000
ELISA 1/1000 1/5000
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Radioimmunoassays
Western Blotting 1/1000 1/5000
Flow Cytometry 1/100 1/1000
Immunofluorescence
Immunofluorescence 1/100 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

References for V5-Tag antibody

  1. Southern, J.A. et al. (1991) Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics.
    J Gen Virol. 72 ( Pt 7): 1551-7.
  2. Orime, K. et al. (2013) Trefoil Factor 2 Promotes Cell Proliferation in Pancreatic β-Cells through CXCR-4-Mediated ERK1/2 Phosphorylation.
    Endocrinology. 154: 54-64.
  3. Randall, R.E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines.
    Vaccine. 11 (12): 1247-52.
  4. Randall, R.E. et al. (1994) Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming.
    Vaccine. 12 (4): 351-8.
  5. Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding.
    J Virol Methods. 53 (1): 149-56.
  6. Jaffray, E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65.
    Mol Cell Biol. 15 (4): 2166-72.
  7. Rodriguez, M.S. et al. (1995) Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein.
    Mol Cell Biol. 15 (5): 2413-9.
  8. Arenzana-Seisdedos, F. et al. (1995) Inducible nuclear expression of newly synthesized I kappa B alpha negatively regulates DNA-binding and transcriptional activities of NF-kappa B.
    Mol Cell Biol. 15 (5): 2689-96.
  9. Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal.
    EMBO J. 13 (22): 5410-20.
  10. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
    J Immunol Methods. 224 (1-2): 141-50.
  11. Young, D.F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells.
    J Virol. 75 (7): 3363-70.
  12. Sanchez Garcia, J. et al. (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit.
    Nucleic Acids Res. 32 (10): 3005-16.
  13. Herskowitz, J.H. et al. (2011) Rho kinase II phosphorylation of the lipoprotein receptor LR11/SORLA alters amyloid-beta production.
    J Biol Chem. 286 (8): 6117-27.
  14. Liebau, M.C. et al. (2011) Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia.
    J Biol Chem. 286: 14237-45.
  15. Björk, J.K. et al. (2010) miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis.
    Development. 137(19):3177-84.
  16. Boggio, R. et al. (2007) Targeting SUMO E1 to ubiquitin ligases: a viral strategy to counteract sumoylation.
    J Biol Chem. 282: 15376-82.
  17. Gallazzini, M. et al. (2011) High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization.
    Mol Biol Cell. 22: 703-14.
  18. Hadler, K.S. et al. (2008) Identification of a non-purple tartrate-resistant acid phosphatase: an evolutionary link to Ser/Thr protein phosphatases?
    BMC Res Notes. 1: 78.
  19. Zhao, A. et al (2011) Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform.
    J Immunol Methods. 363: 221-32.
  20. Patino, G.A. et al. (2011) Voltage-Gated Na+ Channel {beta}1B: A Secreted Cell Adhesion Molecule Involved in Human Epilepsy.
    J Neurosci. 31: 14577-91.
  21. Gatherer, D. et al. (2011) High-resolution human cytomegalovirus transcriptome.
    Proc Natl Acad Sci U S A.108: 19755-60.
  22. Mahuzier, A. et al. (2012) Dishevelled stabilization by the ciliopathy protein Rpgrip1l is essential for planar cell polarity.
    J Cell Biol. 198: 927-40.
  23. Zhao, C. et al. (2005) Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
    Proc Natl Acad Sci U S A. 102:10200-5
  24. Singh, A. et al. (2014) Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks.
    Nucleic Acids Res. 42: 4652-68.
  25. Mui, M.Z. et al. (2015) The Human Adenovirus Type 5 E4orf4 Protein Targets Two Phosphatase Regulators of the Hippo Signaling Pathway.
    J Virol. 89 (17): 8855-70.
  26. Shi X et al. (2016) Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase.
    Proc Natl Acad Sci U S A. 113 (31): 8825-30.
  27. Ng, M.Y. et al. (2017) Activation of MAPK/ERK signaling by Burkholderia pseudomallei cycle inhibiting factor (Cif).
    PLoS One. 12 (2): e0171464.
  28. Voskarides, K. et al. (2017) A functional variant in NEPH3 gene confers high risk of renal failure in primary hematuric glomerulopathies. Evidence for predisposition to microalbuminuria in the general population.
    PLoS One. 12 (3): e0174274.
  29. Malik, S. et al. (2015) Adrenocorticotropic Hormone (ACTH) Responses Require Actions of the Melanocortin-2 Receptor Accessory Protein on the Extracellular Surface of the Plasma Membrane.
    J Biol Chem. 290 (46): 27972-85.
  30. Carrocci, T.J. et al. (2017) SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.
    Nucleic Acids Res. 45 (8): 4837-4852.
CiteAb logo - trusted, tested, published

Our V5-Tag (SV5-Pk1) Antibody has been referenced in >130 publications*


*Based on February 2018 data from CiteAb's antibody search engine.