HA-Tag antibody
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Rabbit anti HA-Tag antibody recognizes the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors.
Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting.
In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins.
Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting.
In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Haemagglutinin epitope were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)Pack Size: 50 µg
0.09% Sodium Azide (NaN3) - Immunogen
- Keyhole limpet hemocyanin conjugated epitope tag peptide YPYDVPDYA (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q03909 Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0019031 viral envelope
- GO:0019064 viral envelope fusion with host membrane
- GO:0020002 host cell plasma membrane
- GO:0046789 host cell surface receptor binding
- GO:0055036 virion membrane
- Regulatory
- For research purposes only
Applications of HA-Tag antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Field, J. et al. (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
Mol Cell Biol. 8 (5): 2159-65. -
Wilson, I.A. et al. (1984) The structure of an antigenic determinant in a protein.
Cell. 37 (3): 767-78.
