Strep-Tag Immo antibody | Strep-MAB

Mouse anti Strep-Tag Immo

Product Type
Monoclonal Antibody
Clone
Strep-MAB
Isotype
IgG1
Specificity
Strep-Tag Immo

Product Code Applications Pack Size List Price Your Price Qty
MCA2488
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Strep-tag Immo antibody, clone Strep-MAB, also known as StrepMAB-Immo, recognizes Strep-tag II sequence WSHPQFEK, a widely used tag in protein expression applications.

This antibody demonstrates near irreversible binding of Strep-tag II, which is N-terminally extended by SerAla making the antibody particularly suited to use in assays such as ELISA and BiaCore.

This product is not recommended for the detection of Strep II fusion proteins in Western blot.

Target Species
Synthetic Peptide
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml PBS.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Ammonium bicarbonate
1% Sucrose
Preservative Stabilisers
None present
Immunogen
Recombinant protein SA-WSHPQFEK.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
Sold under license of IBA

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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RRID
AB_609794