c-Myc antibody | JAC6
Rat anti c-myc antibody, clone JAC6 (MCA1929) used for the detection of c-myc tagged APP by immunofluorescence.
Image caption:
APP processing deficient mutants show stronger synaptogenic activity than APPwt. The synaptogenic activity of APP was analyzed in a co-culture assay. HEK293 cells were transiently transfected with APPwt and seeded 24 h later on primary cortical neurons (DIV7). The cells were fixed after 24 h (neurons DIV 8) and analyzed via immunocytochemistry. (A) Cells were stained with TAU1 as axonal marker, Synaptophysin as presynaptic marker and antibody c-myc for APP.
From: Stahl R, Schilling S, Soba P, et al.
Shedding of APP limits its synaptogenic activity and cell adhesion properties.
Front Cell Neurosci. 2014;8:410.
doi:10.3389/fncel.2014.00410
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti c-myc antibody, clone JAC6 (MCA1929) used for the detection of c-myc tagged APP by immunofluorescence.
Image caption:
APP processing deficient mutants show stronger synaptogenic activity than APPwt. The synaptogenic activity of APP was analyzed in a co-culture assay. HEK293 cells were transiently transfected with APPwt and seeded 24 h later on primary cortical neurons (DIV7). The cells were fixed after 24 h (neurons DIV 8) and analyzed via immunocytochemistry. HEK293 cells were transfected with NLG-1 as a positive control, GFP as a negative control, APPwt and APP processing deficient constructs. Cells were stained with antibody MAP2 as a dendritic marker and Synaptophysin or SV2 as a marker for presynaptic vesicles and antibody c-myc to visualize heterologously expressed APP.
From: Stahl R, Schilling S, Soba P, et al.
Shedding of APP limits its synaptogenic activity and cell adhesion properties.
Front Cell Neurosci. 2014;8:410.
doi:10.3389/fncel.2014.00410
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human c-myc antibody, clone JAC6 (MCA1929) used to evaluate c-myc tagged protein by western blotting.
Image caption:
Analysis of dimer formation of APP WT and APP FAD mutants. A HEK293T cells were transiently transfected with N-terminally c-myc-tagged APP WT, Arctic, Flemish, Iberian, Iowa or Swedish. Cell lysates were prepared, and equal amounts of protein were analyzed via BN-PAGE and subsequent western blot detection. APP and the FAD mutants were detected with an anti-c-myc antibody. Full length APP and monomers/dimers are indicated with arrows.
B BN-PAGE quantification. The relative dimer intensity represents the ratio of APP dimer intensity to the total APP signal intensity (APP dimer plus monomer). Bars represent mean values ± SEM; n = 3 (three biological replicates). Statistical analysis using one way ANOVA with Tukey`s post hoc test did not reveal significant differences
From: Schilling S, Pradhan A, Heesch A, Helbig A, Blennow K, Koch C, Bertgen L, Koo EH, Brinkmalm G, Zetterberg H, Kins S, Eggert S.
Differential effects of familial Alzheimer's disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity.
Acta Neuropathol Commun. 2023 Jun 1;11(1):87.
doi: 10.1186/s40478-023-01577-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human c-myc antibody, clone JAC6 (MCA1929) used to evaluate c-myc tagged protein by western blotting.
Image caption:
Presence of APP WT and APP FAD mutants at the cell surface.
C Cell surface biotinylation of transiently APP WT, Swedish, Arctic, Flemish, Iowa or Iberian transfected HEK293T cells. Direct load of cell lysates is shown in the left panel. In the right panel, APP cell surface levels after streptavidin immunoprecipitation and Western blot detection with anti-c-myc antibody are shown. β-Actin served as a loading control and was detected in the IP samples only to a minor extent, which confirms specificity of the pull down of surface proteins. The lower panel shows a higher exposure time.
From: Schilling S, Pradhan A, Heesch A, Helbig A, Blennow K, Koch C, Bertgen L, Koo EH, Brinkmalm G, Zetterberg H, Kins S, Eggert S.
Differential effects of familial Alzheimer's disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity.
Acta Neuropathol Commun. 2023 Jun 1;11(1):87.
doi: 10.1186/s40478-023-01577-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human c-myc antibody, clone JAC6 (MCA1929) used to evaluate c-myc tagged protein by immunofluorescence.
Image caption:
Presence of APP WT and APP FAD mutants at the cell surface. A Cell surface staining. HeLa cells were transiently transfected with N-terminally c-myc-tagged APP WT, Swedish, Arctic, Flemish, Iowa or Iberian. Cells were labeled with α-c-myc antibody (green, surface APP; red, total APP). The images were taken with an epifluorescence microscope including apotome. Scale bar: 20 μm.
From: Schilling S, Pradhan A, Heesch A, Helbig A, Blennow K, Koch C, Bertgen L, Koo EH, Brinkmalm G, Zetterberg H, Kins S, Eggert S.
Differential effects of familial Alzheimer's disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity.
Acta Neuropathol Commun. 2023 Jun 1;11(1):87.
doi: 10.1186/s40478-023-01577-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human c-myc antibody, clone JAC6 (MCA1929) used to evaluate c-myc tagged protein by immunofluorescence.
Image caption:
Colocalization of APP WT and APP FAD mutants in the cis Golgi apparatus.
A HeLa cells were transiently transfected with N-terminally c-myc-tagged APP WT, Swedish, Arctic, Flemish, Iowa or Iberian. Cells were labeled with α-c-myc and α-GM130 (marker for Golgi apparatus) antibodies. The merge reveals colocalization of both channels, indicated in yellow. Scale bar: 20 μm.
From: Schilling S, Pradhan A, Heesch A, Helbig A, Blennow K, Koch C, Bertgen L, Koo EH, Brinkmalm G, Zetterberg H, Kins S, Eggert S.
Differential effects of familial Alzheimer's disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity.
Acta Neuropathol Commun. 2023 Jun 1;11(1):87.
doi: 10.1186/s40478-023-01577-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human c-myc antibody, clone JAC6 (MCA1929) used to evaluate c-myc tagged protein by immunofluorescence.
Image caption:
Colocalization of APP WT and APP FAD mutants in Early Endosomes.
A HeLa cells were transiently transfected with N-terminally c-myc-tagged APP WT, Swedish, Arctic, Flemish, Iowa or Iberian. Cells were labeled with α-c-myc and α-EEA1 (marker for early endosomes) antibodies. The merge reveals colocalization of both channels, indicated in yellow. Scale bar: 20 μm.
From: Schilling S, Pradhan A, Heesch A, Helbig A, Blennow K, Koch C, Bertgen L, Koo EH, Brinkmalm G, Zetterberg H, Kins S, Eggert S.
Differential effects of familial Alzheimer's disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity.
Acta Neuropathol Commun. 2023 Jun 1;11(1):87.
doi: 10.1186/s40478-023-01577-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti c-Myc
- Product Type
- Monoclonal Antibody
- Clone
- JAC6
- Isotype
- IgG1
- Specificity
- c-Myc
Antibody Quality and Performance Guarantee
| Rat anti Human c-myc antibody, clone JAC6 recognises the myc proto-oncogene which is frequently used to tag proteins, and is the same epitope recognized by clone 9E10. c-myc is also known as Transcription factor p64 or Class E basic helix-loop-helix protein 39. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Amino acids 408-439 corresponding to the C-terminal region of human c-myc.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for c-Myc antibody
-
Smith, G.D. et al. (2002) TRPV3 is a temperature-sensitive vanilloid receptor-like protein.
Nature. 418 (6894): 186-90. -
Shin, Y. et al. (2005) The co-chaperone carboxyl terminus of Hsp70-interacting protein (CHIP) mediates alpha-synuclein degradation decisions between proteasomal and lysosomal pathways.
J Biol Chem. 280 (25): 23727-34. -
Lozano, E. et al. (2008) PAK is required for the disruption of E-cadherin adhesion by the small GTPase Rac.
J Cell Sci. 121 (Pt 7): 933-8. -
Tetzlaff, J.E. et al. (2008) CHIP targets toxic alpha-Synuclein oligomers for degradation.
J Biol Chem. 283 (26): 17962-8. -
Dawson, K. et al. (2008) Loss of regulators of vacuolar ATPase function and ceramide synthesis results in multidrug sensitivity in Schizosaccharomyces pombe.
Eukaryot Cell. 7 (6): 926-37. -
Jenkins, P.M. et al. (2009) PACS-1 mediates phosphorylation-dependent ciliary trafficking of the cyclic-nucleotide-gated channel in olfactory sensory neurons.
J Neurosci. 29 (34): 10541-51. -
Liebig, T. et al. (2009) RhoE Is required for keratinocyte differentiation and stratification.
Mol Biol Cell. 20 (1): 452-63. -
Stahl, R. et al. (2014) Shedding of APP limits its synaptogenic activity and cell adhesion properties.
Front Cell Neurosci. 8: 410.
View The Latest Product References
-
Smith, M.D. et al. (2018) CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.
Dev Cell. 44 (2): 217-232.e11. -
Tsuboguchi, S. et al. (2023) TDP-43 differentially propagates to induce antero- and retrograde degeneration in the corticospinal circuits in mouse focal ALS models.
Acta Neuropathol. 146 (4): 611-629. -
Schilling, S. et al. (2023) Differential effects of familial Alzheimer's disease-causing mutations on amyloid precursor protein (APP) trafficking, proteolytic conversion, and synaptogenic activity.
Acta Neuropathol Commun. 11 (1): 87.
- RRID
- AB_322203
- UniProt
- P01106
- Entrez Gene
- MYC
- GO Terms
- GO:0005515 protein binding
- GO:0001658 branching involved in ureteric bud morphogenesis
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006357 regulation of transcription from RNA polymerase II promoter
- GO:0006879 cellular iron ion homeostasis
- GO:0007050 cell cycle arrest
- GO:0008283 cell proliferation
- View More GO Terms
- GO:0042493 response to drug
- GO:0016563 transcription activator activity
- GO:0032204 regulation of telomere maintenance
- GO:0070888 E-box binding
- GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation
MCA1929
153313
163384
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