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c-Myc antibody | JAC6

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Anti c-Myc Antibody, clone JAC6 thumbnail image 1 Anti c-Myc Antibody, clone JAC6 thumbnail image 2
Anti c-Myc Antibody, clone JAC6 gallery image 1
Published customer image:
Rat anti c-myc antibody, clone JAC6 (MCA1929) used for the detection of c-myc tagged APP by immunofluorescence.
Image caption:
APP processing deficient mutants show stronger synaptogenic activity than APPwt. The synaptogenic activity of APP was analyzed in a co-culture assay. HEK293 cells were transiently transfected with APPwt and seeded 24 h later on primary cortical neurons (DIV7). The cells were fixed after 24 h (neurons DIV 8) and analyzed via immunocytochemistry. (A) Cells were stained with TAU1 as axonal marker, Synaptophysin as presynaptic marker and antibody c-myc for APP.

From: Stahl R, Schilling S, Soba P, et al.
Shedding of APP limits its synaptogenic activity and cell adhesion properties.
Front Cell Neurosci. 2014;8:410.
doi:10.3389/fncel.2014.00410
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti c-Myc Antibody, clone JAC6 gallery image 2
Published customer image:
Rat anti c-myc antibody, clone JAC6 (MCA1929) used for the detection of c-myc tagged APP by immunofluorescence.
Image caption:
APP processing deficient mutants show stronger synaptogenic activity than APPwt. The synaptogenic activity of APP was analyzed in a co-culture assay. HEK293 cells were transiently transfected with APPwt and seeded 24 h later on primary cortical neurons (DIV7). The cells were fixed after 24 h (neurons DIV 8) and analyzed via immunocytochemistry. HEK293 cells were transfected with NLG-1 as a positive control, GFP as a negative control, APPwt and APP processing deficient constructs. Cells were stained with antibody MAP2 as a dendritic marker and Synaptophysin or SV2 as a marker for presynaptic vesicles and antibody c-myc to visualize heterologously expressed APP.

From: Stahl R, Schilling S, Soba P, et al.
Shedding of APP limits its synaptogenic activity and cell adhesion properties.
Front Cell Neurosci. 2014;8:410.
doi:10.3389/fncel.2014.00410
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti c-Myc

Product Type
Monoclonal Antibody
Clone
JAC6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1929
Datasheet
C IF IP WB
SDS
0.1 mg loader

Rat anti Human c-myc antibody, clone JAC6 recognises the myc proto-oncogene which is frequently used to tag proteins, and is the same epitope recognised by clone 9E10. c-myc is also known as Transcription factor p64 orClass E basic helix-loop-helix protein 39.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Amino acids 408-439 corresponding to the C-terminal region of human c-myc.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01106
Entrez Gene
MYC
GO Terms
GO:0005515 protein binding
GO:0001658 branching involved in ureteric bud morphogenesis
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006879 cellular iron ion homeostasis
GO:0007050 cell cycle arrest
GO:0008283 cell proliferation
GO:0042493 response to drug
GO:0016563 transcription activator activity
GO:0032204 regulation of telomere maintenance
GO:0070888 E-box binding
GO:0090096 positive regulation of metanephric cap mesenchymal cell proliferation
Regulatory
For research purposes only

Applications of c-Myc antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Application Based External Images

Immunofluorescence

Immunoprecipitation

Western Blotting

Product Specific References

References for c-Myc antibody

  1. Smith, G.D. et al. (2002) TRPV3 is a temperature-sensitive vanilloid receptor-like protein.
    Nature. 418 (6894): 186-90.
  2. Dawson, K. et al. (2008) Loss of regulators of vacuolar ATPase function and ceramide synthesis results in multidrug sensitivity in Schizosaccharomyces pombe.
    Eukaryot Cell. 7 (6): 926-37.
  3. Tetzlaff, J.E. et al. (2008) CHIP targets toxic alpha-Synuclein oligomers for degradation.
    J Biol Chem. 283 (26): 17962-8.
  4. Shin, Y. et al. (2005) The co-chaperone carboxyl terminus of Hsp70-interacting protein (CHIP) mediates alpha-synuclein degradation decisions between proteasomal and lysosomal pathways.
    J Biol Chem. 280 (25): 23727-34.
  5. Liebig, T. et al. (2009) RhoE Is required for keratinocyte differentiation and stratification.
    Mol Biol Cell. 20 (1): 452-63.
  6. Lozano, E. et al. (2008) PAK is required for the disruption of E-cadherin adhesion by the small GTPase Rac.
    J Cell Sci. 121 (Pt 7): 933-8.
  7. Stahl, R. et al. (2014) Shedding of APP limits its synaptogenic activity and cell adhesion properties.
    Front Cell Neurosci. 8: 410.
  8. Smith, M.D. et al. (2018) CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.
    Dev Cell. 44 (2): 217-232.e11.
  9. Jenkins, P.M. et al. (2009) PACS-1 mediates phosphorylation-dependent ciliary trafficking of the cyclic-nucleotide-gated channel in olfactory sensory neurons.
    J Neurosci. 29 (34): 10541-51.
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