Green Fluorescent Protein antibody

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Sheep anti Green Fluorescent Protein

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4745-1051 E IF 1 ml
Sheep anti Green Fluorescent Protein antibody recognizes green fluorescent protein (GFP), a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression.

Product Details

Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Green fluorescent protein from Aequorea victoria.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P42212 Related reagents
GO Terms
GO:0006091 generation of precursor metabolites and energy
GO:0008218 bioluminescence
GO:0018298 protein-chromophore linkage
Regulatory
For research purposes only

Applications of Green Fluorescent Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti mCherry AHP2326 50 µg WB
Rabbit anti Blue Fluorescent Protein AHP2985 50 µg WB
Rabbit anti Cyan Fluorescent Protein AHP2986 50 µg WB
Rabbit anti Red Fluorescent Protein AHP2987 50 µg WB
Mouse anti mCherry MCA6020 50 µg WB

Application Based External Images

ELISA

Immunofluorescence

Product Specific References

References for Green Fluorescent Protein antibody

  1. Collins, R.T. et al. (2010) MAZe: a tool for mosaic analysis of gene function in zebrafish.
    Nat Methods. 7: 219-23.
  2. Wu, L. et al. (2011) Properties of a distinct subpopulation of GABAergic commissural interneurons that are part of the locomotor circuitry in the neonatal spinal cord.
    J Neurosci. 31 (13): 4821-33.
  3. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
    Blood. 116: 2183-91.
  4. Shneider, N.A. et al. (2009) Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival.
    Neural Dev. 4: 42.
  5. Soza-Ried, C. et al. (2008) Maintenance of thymic epithelial phenotype requires extrinsic signals in mouse and zebrafish.
    J Immunol. 181: 5272-7.
  6. Lopez, K.A. et al. (2011) Convection-enhanced delivery of topotecan into a PDGF-driven model of glioblastoma prolongs survival and ablates both tumor-initiating cells and recruited glial progenitors.
    Cancer Res. 71: 3963-71.
  7. League, G.P. and Nam, S.C. (2011) Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongation in Drosophila photoreceptor.
    PLoS One. 6:e21218.
  8. Siembab, V.C. et al. (2010) Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells.
    J Comp Neurol. 518: 4675-701.
  9. Srinivasan, S. et al. (2012) The receptor tyrosine phosphatase Lar regulates adhesion between Drosophila male germline stem cells and the niche.
    Development. 139: 1381-90.
  10. Haberlandt, C. et al. (2011) Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
    PLoS One. 6: e17575.
  11. Cheung, L.S. et al. (2013) Dynamic model for the coordination of two enhancers of broad by EGFR signaling.
    Proc Natl Acad Sci U S A. 110: 17939-44.
  12. Li, X. et al. (2013) Temporal patterning of Drosophila medulla neuroblasts controls neural fates.
    Nature. 498: 456-62.
  13. Behnia, R. et al. (2014) Processing properties of ON and OFF pathways for Drosophila motion detection.
    Nature. 512: 427-30.
  14. de Nooij, J.C. et al. (2015) The PDZ-domain protein Whirlin facilitates mechanosensory signaling in mammalian proprioceptors.
    J Neurosci. 35 (7): 3073-84.
  15. Scotti, M. et al. (2015) A Hoxa13:Cre mouse strain for conditional gene manipulation in developing limb, hindgut, and urogenital system.
    Genesis. 53 (6): 366-76.
  16. Sun, G.J. et al. (2015) Latent tri-lineage potential of adult hippocampal neural stem cells revealed by Nf1 inactivation.
    Nat Neurosci. 18 (12): 1722-4.
  17. Schlegel, P. et al. (2016) Synaptic transmission parallels neuromodulation in a central food-intake circuit.
    eLife 2016;10.7554/eLife.16799
  18. Crouch, E.E. et al. (2015) Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.
    J Neurosci. 35 (11): 4528-39.

Further Reading

  1. Adams, K.L. et al. (2015) Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
    Nat Commun. 6: 6778.