V5-Tag antibody | SV5-Pk4

Mouse anti V5-Tag

Product Type
Monoclonal Antibody
Clone
SV5-Pk4
Isotype
IgG1
Specificity
V5-Tag

Product Code Applications Pack Size List Price Your Price Qty
MCA2894GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF IP WB 0.1 mg loader
List Price Your Price
loader
MCA2894
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF IP WB 1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti V5-Tag antibody, clone SV5-Pk4 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk4 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is manufactued under an exclusive license from the University of St. Andrews, UK.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunoprecipitation
Western Blotting 1/100 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for V5-Tag antibody

  1. Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
    J Immunol Methods. 224 (1-2): 141-50.

Synonyms
PK-TAG
RRID
AB_1658045
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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