V5-Tag antibody | SV5-Pk4

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Anti V5-Tag Antibody, clone SV5-Pk4 thumbnail image 1

Anti V5-Tag Antibody, clone SV5-Pk4 thumbnail image 2

Anti V5-Tag Antibody, clone SV5-Pk4 gallery image 1 — V5 tagged protein detected with Mouse anti V5-tag (MCA2894)

Anti V5-Tag Antibody, clone SV5-Pk4 gallery image 2 — **Western Blot** analysis of 1 µg V5 tagged HuCAL Fab-A-V5Sx2 loaded into lanes 2-6. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 7. Mouse anti V5-tag antibody (**MCA2894F**) was used at a dilution of 1/200 in lane 2, 1/1000 in lane 4 and at 1/2000 in lane 5. Membrane visualized on the ChemiDoc™ MP Imaging System.

Mouse anti V5-Tag:FITC

Mouse anti V5-Tag

Mouse anti V5-Tag:DyLight®549

Mouse anti V5-Tag:DyLight®649

Product Type: Monoclonal Antibody
Clone: SV5-Pk4
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2894F	F IF WB	0.1 mg
MCA2894GA	E IF IP WB	0.1 mg
MCA2894	E IF IP WB	1 mg
MCA2894D549GA	F IF WB	0.1 mg
MCA2894D649GA	F IF WB	0.1 mg

Mouse anti V5-Tag antibody, clone SV5-Pk4 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk4 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).

Product Details

Target Species

Viral

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to DyLight^®549 - liquid

Product Form

Purified IgG conjugated to DyLight^®649 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.

More Information

Acknowledgements: This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Acknowledgements: This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Regulatory: For research purposes only

Applications of V5-Tag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/5
Immunofluorescence
Western Blotting
ELISA
Immunofluorescence
Immunoprecipitation
Western Blotting	1/100	1/500
Flow Cytometry	Neat	1/5
Immunofluorescence
Western Blotting
Flow Cytometry	Neat	1/5
Immunofluorescence
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for V5-Tag antibody

Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
J Immunol Methods. 224 (1-2): 141-50.

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